Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16595 and SGL_RS20175 are separated by 66 nucleotides SGL_RS20175 and SGL_RS16600 are separated by 46 nucleotides SGL_RS16600 and SGL_RS16605 are separated by 293 nucleotides SGL_RS16605 and SGL_RS16610 are separated by 301 nucleotides
SGL_RS16595: SGL_RS16595 - NAD(P)H-quinone oxidoreductase subunit F, at 3,178,231 to 3,180,135
_RS16595
SGL_RS20175: SGL_RS20175 - hypothetical protein, at 3,180,202 to 3,180,333
_RS20175
SGL_RS16600: SGL_RS16600 - hypothetical protein, at 3,180,380 to 3,181,414
_RS16600
SGL_RS16605: SGL_RS16605 - RNA-binding protein, at 3,181,708 to 3,182,013
_RS16605
SGL_RS16610: SGL_RS16610 - magnesium protoporphyrin IX methyltransferase, at 3,182,315 to 3,183,007
_RS16610
Position (kb)
3180
3181
3182 Strain fitness (log2 ratio)
-1
0
1 at 3179.389 kb on + strand, within SGL_RS16595 at 3179.556 kb on - strand, within SGL_RS16595 at 3179.564 kb on - strand, within SGL_RS16595 at 3179.621 kb on + strand, within SGL_RS16595 at 3179.624 kb on + strand, within SGL_RS16595 at 3179.666 kb on - strand, within SGL_RS16595 at 3179.694 kb on + strand, within SGL_RS16595 at 3179.788 kb on + strand, within SGL_RS16595 at 3179.796 kb on - strand, within SGL_RS16595 at 3179.796 kb on - strand, within SGL_RS16595 at 3179.810 kb on - strand, within SGL_RS16595 at 3179.904 kb on - strand, within SGL_RS16595 at 3180.024 kb on + strand at 3180.066 kb on + strand at 3180.290 kb on - strand, within SGL_RS20175 at 3180.411 kb on + strand at 3180.519 kb on - strand, within SGL_RS16600 at 3180.662 kb on - strand, within SGL_RS16600 at 3181.574 kb on + strand at 3181.825 kb on + strand, within SGL_RS16605 at 3181.988 kb on - strand at 3182.121 kb on + strand at 3182.123 kb on + strand at 3182.156 kb on + strand at 3182.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 3,179,389 + SGL_RS16595 0.61 +0.6 3,179,556 - SGL_RS16595 0.70 +0.4 3,179,564 - SGL_RS16595 0.70 +1.0 3,179,621 + SGL_RS16595 0.73 +1.5 3,179,624 + SGL_RS16595 0.73 -0.1 3,179,666 - SGL_RS16595 0.75 -0.6 3,179,694 + SGL_RS16595 0.77 +0.2 3,179,788 + SGL_RS16595 0.82 +0.2 3,179,796 - SGL_RS16595 0.82 -0.6 3,179,796 - SGL_RS16595 0.82 +0.5 3,179,810 - SGL_RS16595 0.83 -0.3 3,179,904 - SGL_RS16595 0.88 +0.0 3,180,024 + +0.3 3,180,066 + +0.6 3,180,290 - SGL_RS20175 0.67 +0.3 3,180,411 + -1.3 3,180,519 - SGL_RS16600 0.13 +1.6 3,180,662 - SGL_RS16600 0.27 +0.8 3,181,574 + -0.5 3,181,825 + SGL_RS16605 0.38 -0.4 3,181,988 - -0.3 3,182,121 + +0.4 3,182,123 + -0.1 3,182,156 + -1.2 3,182,177 + +0.8
Or see this region's nucleotide sequence