Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15315 and SGL_RS15320 are separated by 130 nucleotides SGL_RS15320 and SGL_RS15325 are separated by 142 nucleotides
SGL_RS15315: SGL_RS15315 - 30S ribosomal protein S12 methylthiotransferase RimO, at 2,886,182 to 2,887,513
_RS15315
SGL_RS15320: SGL_RS15320 - DEAD/DEAH box helicase, at 2,887,644 to 2,889,122
_RS15320
SGL_RS15325: SGL_RS15325 - N-acetyl-gamma-glutamyl-phosphate reductase, at 2,889,265 to 2,890,320
_RS15325
Position (kb)
2887
2888
2889
2890 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2886.747 kb on + strand, within SGL_RS15315 at 2886.827 kb on + strand, within SGL_RS15315 at 2886.874 kb on - strand, within SGL_RS15315 at 2886.880 kb on - strand, within SGL_RS15315 at 2887.072 kb on - strand, within SGL_RS15315 at 2887.218 kb on - strand, within SGL_RS15315 at 2887.312 kb on + strand, within SGL_RS15315 at 2887.320 kb on - strand, within SGL_RS15315 at 2887.419 kb on + strand at 2887.506 kb on - strand at 2887.791 kb on - strand at 2887.982 kb on - strand, within SGL_RS15320 at 2888.002 kb on + strand, within SGL_RS15320 at 2888.010 kb on - strand, within SGL_RS15320 at 2888.035 kb on + strand, within SGL_RS15320 at 2888.035 kb on + strand, within SGL_RS15320 at 2888.136 kb on - strand, within SGL_RS15320 at 2888.266 kb on + strand, within SGL_RS15320 at 2888.276 kb on + strand, within SGL_RS15320 at 2888.599 kb on + strand, within SGL_RS15320 at 2888.682 kb on + strand, within SGL_RS15320 at 2888.706 kb on + strand, within SGL_RS15320 at 2888.831 kb on - strand, within SGL_RS15320 at 2888.897 kb on - strand, within SGL_RS15320 at 2889.062 kb on + strand at 2889.203 kb on - strand at 2889.389 kb on - strand, within SGL_RS15325 at 2889.616 kb on + strand, within SGL_RS15325 at 2890.085 kb on + strand, within SGL_RS15325 at 2890.119 kb on - strand, within SGL_RS15325
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,886,747 + SGL_RS15315 0.42 -2.1 2,886,827 + SGL_RS15315 0.48 -4.2 2,886,874 - SGL_RS15315 0.52 -4.0 2,886,880 - SGL_RS15315 0.52 -3.6 2,887,072 - SGL_RS15315 0.67 -3.7 2,887,218 - SGL_RS15315 0.78 -3.1 2,887,312 + SGL_RS15315 0.85 -3.2 2,887,320 - SGL_RS15315 0.85 -2.4 2,887,419 + -3.8 2,887,506 - -2.7 2,887,791 - -4.6 2,887,982 - SGL_RS15320 0.23 -6.0 2,888,002 + SGL_RS15320 0.24 -3.9 2,888,010 - SGL_RS15320 0.25 -2.2 2,888,035 + SGL_RS15320 0.26 -3.6 2,888,035 + SGL_RS15320 0.26 -3.3 2,888,136 - SGL_RS15320 0.33 -2.3 2,888,266 + SGL_RS15320 0.42 -2.7 2,888,276 + SGL_RS15320 0.43 -3.2 2,888,599 + SGL_RS15320 0.65 -2.3 2,888,682 + SGL_RS15320 0.70 -5.2 2,888,706 + SGL_RS15320 0.72 -1.2 2,888,831 - SGL_RS15320 0.80 +0.8 2,888,897 - SGL_RS15320 0.85 -4.4 2,889,062 + -3.9 2,889,203 - +0.6 2,889,389 - SGL_RS15325 0.12 -1.5 2,889,616 + SGL_RS15325 0.33 -0.9 2,890,085 + SGL_RS15325 0.78 -1.2 2,890,119 - SGL_RS15325 0.81 -1.2
Or see this region's nucleotide sequence