Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14885 and SGL_RS14890 are separated by 37 nucleotides SGL_RS14890 and SGL_RS14895 are separated by 541 nucleotides SGL_RS14895 and SGL_RS14900 overlap by 4 nucleotides SGL_RS14900 and SGL_RS14905 are separated by 65 nucleotides
SGL_RS14885: SGL_RS14885 - malate dehydrogenase, at 2,795,426 to 2,796,400
_RS14885
SGL_RS14890: SGL_RS14890 - NAD(P)H-quinone oxidoreductase subunit O, at 2,796,438 to 2,796,656
_RS14890
SGL_RS14895: SGL_RS14895 - DUF2811 domain-containing protein, at 2,797,198 to 2,797,425
_RS14895
SGL_RS14900: SGL_RS14900 - aminoacyl-tRNA hydrolase, at 2,797,422 to 2,798,006
_RS14900
SGL_RS14905: SGL_RS14905 - 30S ribosomal protein PSRP-3, at 2,798,072 to 2,798,410
_RS14905
Position (kb)
2797
2798 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2796.313 kb on + strand at 2796.349 kb on + strand at 2796.414 kb on - strand at 2796.466 kb on - strand, within SGL_RS14890 at 2796.478 kb on - strand, within SGL_RS14890 at 2796.631 kb on - strand, within SGL_RS14890 at 2796.864 kb on - strand at 2797.160 kb on + strand at 2797.281 kb on - strand, within SGL_RS14895 at 2797.309 kb on + strand, within SGL_RS14895 at 2797.323 kb on - strand, within SGL_RS14895 at 2797.492 kb on - strand, within SGL_RS14900 at 2797.550 kb on - strand, within SGL_RS14900 at 2797.672 kb on - strand, within SGL_RS14900 at 2797.967 kb on + strand at 2798.080 kb on + strand at 2798.133 kb on - strand, within SGL_RS14905 at 2798.178 kb on + strand, within SGL_RS14905 at 2798.280 kb on + strand, within SGL_RS14905 at 2798.309 kb on + strand, within SGL_RS14905
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,796,313 + +0.6 2,796,349 + +0.2 2,796,414 - -0.2 2,796,466 - SGL_RS14890 0.13 +1.5 2,796,478 - SGL_RS14890 0.18 +1.9 2,796,631 - SGL_RS14890 0.88 +1.3 2,796,864 - +1.0 2,797,160 + +0.9 2,797,281 - SGL_RS14895 0.36 +0.8 2,797,309 + SGL_RS14895 0.49 +1.1 2,797,323 - SGL_RS14895 0.55 +0.9 2,797,492 - SGL_RS14900 0.12 -0.1 2,797,550 - SGL_RS14900 0.22 -1.4 2,797,672 - SGL_RS14900 0.43 -0.7 2,797,967 + +0.8 2,798,080 + -2.8 2,798,133 - SGL_RS14905 0.18 -2.7 2,798,178 + SGL_RS14905 0.31 -4.5 2,798,280 + SGL_RS14905 0.61 -2.3 2,798,309 + SGL_RS14905 0.70 -1.7
Or see this region's nucleotide sequence