Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14505 and SGL_RS14510 are separated by 125 nucleotides SGL_RS14510 and SGL_RS14515 are separated by 135 nucleotides SGL_RS14515 and SGL_RS14520 overlap by 4 nucleotides SGL_RS14520 and SGL_RS14525 are separated by 102 nucleotides
SGL_RS14505: SGL_RS14505 - glutamate-5-semialdehyde dehydrogenase, at 2,709,084 to 2,710,385
_RS14505
SGL_RS14510: SGL_RS14510 - DUF3177 family protein, at 2,710,511 to 2,711,107
_RS14510
SGL_RS14515: SGL_RS14515 - type II toxin-antitoxin system HicA family toxin, at 2,711,243 to 2,711,422
_RS14515
SGL_RS14520: SGL_RS14520 - type II toxin-antitoxin system HicB family antitoxin, at 2,711,419 to 2,711,637
_RS14520
SGL_RS14525: SGL_RS14525 - reverse transcriptase domain-containing protein, at 2,711,740 to 2,713,305
_RS14525
Position (kb)
2710
2711
2712 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2709.540 kb on + strand, within SGL_RS14505 at 2709.554 kb on - strand, within SGL_RS14505 at 2709.649 kb on + strand, within SGL_RS14505 at 2709.671 kb on - strand, within SGL_RS14505 at 2709.745 kb on - strand, within SGL_RS14505 at 2710.087 kb on - strand, within SGL_RS14505 at 2710.178 kb on - strand, within SGL_RS14505 at 2710.380 kb on + strand at 2710.439 kb on - strand at 2710.489 kb on + strand at 2710.497 kb on - strand at 2710.650 kb on + strand, within SGL_RS14510 at 2710.652 kb on + strand, within SGL_RS14510 at 2710.660 kb on - strand, within SGL_RS14510 at 2710.722 kb on - strand, within SGL_RS14510 at 2711.162 kb on + strand at 2711.455 kb on + strand, within SGL_RS14520 at 2711.505 kb on - strand, within SGL_RS14520 at 2712.000 kb on - strand, within SGL_RS14525
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,709,540 + SGL_RS14505 0.35 -3.3 2,709,554 - SGL_RS14505 0.36 -3.6 2,709,649 + SGL_RS14505 0.43 -1.1 2,709,671 - SGL_RS14505 0.45 -2.4 2,709,745 - SGL_RS14505 0.51 -1.9 2,710,087 - SGL_RS14505 0.77 -0.1 2,710,178 - SGL_RS14505 0.84 -4.2 2,710,380 + -1.0 2,710,439 - +0.3 2,710,489 + +0.1 2,710,497 - +0.3 2,710,650 + SGL_RS14510 0.23 -4.6 2,710,652 + SGL_RS14510 0.24 -3.6 2,710,660 - SGL_RS14510 0.25 -4.0 2,710,722 - SGL_RS14510 0.35 -1.1 2,711,162 + -3.6 2,711,455 + SGL_RS14520 0.16 +0.4 2,711,505 - SGL_RS14520 0.39 -0.7 2,712,000 - SGL_RS14525 0.17 -1.5
Or see this region's nucleotide sequence