Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14480 and SGL_RS14485 are separated by 195 nucleotides SGL_RS14485 and SGL_RS14490 are separated by 147 nucleotides
SGL_RS14480: SGL_RS14480 - uroporphyrinogen-III C-methyltransferase, at 2,700,944 to 2,701,735
_RS14480
SGL_RS14485: SGL_RS14485 - transcription-repair coupling factor, at 2,701,931 to 2,705,530
_RS14485
SGL_RS14490: SGL_RS14490 - hypothetical protein, at 2,705,678 to 2,705,983
_RS14490
Position (kb)
2701
2702
2703
2704
2705
2706 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2700.956 kb on + strand at 2701.126 kb on - strand, within SGL_RS14480 at 2701.729 kb on + strand at 2701.829 kb on + strand at 2702.032 kb on - strand at 2702.034 kb on + strand at 2702.979 kb on - strand, within SGL_RS14485 at 2703.011 kb on + strand, within SGL_RS14485 at 2703.115 kb on + strand, within SGL_RS14485 at 2703.222 kb on + strand, within SGL_RS14485 at 2703.462 kb on + strand, within SGL_RS14485 at 2703.508 kb on + strand, within SGL_RS14485 at 2703.518 kb on - strand, within SGL_RS14485 at 2703.566 kb on + strand, within SGL_RS14485 at 2704.021 kb on - strand, within SGL_RS14485 at 2704.253 kb on + strand, within SGL_RS14485 at 2704.538 kb on - strand, within SGL_RS14485 at 2704.605 kb on - strand, within SGL_RS14485 at 2704.608 kb on - strand, within SGL_RS14485 at 2704.819 kb on - strand, within SGL_RS14485 at 2704.887 kb on + strand, within SGL_RS14485 at 2704.889 kb on + strand, within SGL_RS14485 at 2704.909 kb on + strand, within SGL_RS14485 at 2704.956 kb on - strand, within SGL_RS14485 at 2705.067 kb on + strand, within SGL_RS14485 at 2705.067 kb on + strand, within SGL_RS14485 at 2705.075 kb on - strand, within SGL_RS14485 at 2705.085 kb on - strand, within SGL_RS14485 at 2705.145 kb on + strand, within SGL_RS14485 at 2705.169 kb on - strand, within SGL_RS14485 at 2705.225 kb on - strand at 2705.301 kb on - strand at 2705.385 kb on - strand at 2705.423 kb on + strand at 2705.423 kb on + strand at 2705.431 kb on - strand at 2705.436 kb on - strand at 2705.514 kb on + strand at 2705.514 kb on + strand at 2705.514 kb on + strand at 2705.522 kb on - strand at 2705.522 kb on - strand at 2705.522 kb on - strand at 2705.522 kb on - strand at 2705.522 kb on - strand at 2705.780 kb on + strand, within SGL_RS14490 at 2705.788 kb on - strand, within SGL_RS14490 at 2705.897 kb on - strand, within SGL_RS14490 at 2705.977 kb on + strand at 2706.088 kb on + strand at 2706.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,700,956 + +0.1 2,701,126 - SGL_RS14480 0.23 -0.7 2,701,729 + -3.0 2,701,829 + -0.8 2,702,032 - +2.3 2,702,034 + +2.4 2,702,979 - SGL_RS14485 0.29 +3.2 2,703,011 + SGL_RS14485 0.30 +2.9 2,703,115 + SGL_RS14485 0.33 +3.4 2,703,222 + SGL_RS14485 0.36 +3.3 2,703,462 + SGL_RS14485 0.43 +2.7 2,703,508 + SGL_RS14485 0.44 +2.7 2,703,518 - SGL_RS14485 0.44 +3.5 2,703,566 + SGL_RS14485 0.45 +2.0 2,704,021 - SGL_RS14485 0.58 +3.1 2,704,253 + SGL_RS14485 0.65 +3.0 2,704,538 - SGL_RS14485 0.72 +2.5 2,704,605 - SGL_RS14485 0.74 +3.1 2,704,608 - SGL_RS14485 0.74 +3.4 2,704,819 - SGL_RS14485 0.80 +3.2 2,704,887 + SGL_RS14485 0.82 +2.6 2,704,889 + SGL_RS14485 0.82 +3.3 2,704,909 + SGL_RS14485 0.83 +2.8 2,704,956 - SGL_RS14485 0.84 +3.5 2,705,067 + SGL_RS14485 0.87 +3.3 2,705,067 + SGL_RS14485 0.87 +3.2 2,705,075 - SGL_RS14485 0.87 +3.4 2,705,085 - SGL_RS14485 0.88 +2.7 2,705,145 + SGL_RS14485 0.89 +3.6 2,705,169 - SGL_RS14485 0.90 +3.5 2,705,225 - +3.1 2,705,301 - +3.2 2,705,385 - +2.9 2,705,423 + +1.0 2,705,423 + +0.6 2,705,431 - +1.6 2,705,436 - +1.1 2,705,514 + -0.3 2,705,514 + +0.7 2,705,514 + -0.7 2,705,522 - +0.6 2,705,522 - +0.7 2,705,522 - -0.6 2,705,522 - +0.4 2,705,522 - -0.7 2,705,780 + SGL_RS14490 0.33 +0.2 2,705,788 - SGL_RS14490 0.36 +1.3 2,705,897 - SGL_RS14490 0.72 +0.7 2,705,977 + +0.4 2,706,088 + +1.3 2,706,189 - +0.7
Or see this region's nucleotide sequence