Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13750 and SGL_RS13755 are separated by 112 nucleotides SGL_RS13755 and SGL_RS13760 are separated by 156 nucleotides SGL_RS13760 and SGL_RS13765 are separated by 172 nucleotides SGL_RS13765 and SGL_RS13770 are separated by 153 nucleotides SGL_RS13770 and SGL_RS13775 are separated by 164 nucleotides
SGL_RS13750: SGL_RS13750 - hypothetical protein, at 2,552,177 to 2,552,962
_RS13750
SGL_RS13755: SGL_RS13755 - hypothetical protein, at 2,553,075 to 2,553,338
_RS13755
SGL_RS13760: SGL_RS13760 - PQ-loop repeat-containing protein, at 2,553,495 to 2,553,788
_RS13760
SGL_RS13765: SGL_RS13765 - glycolate oxidase subunit GlcD, at 2,553,961 to 2,555,439
_RS13765
SGL_RS13770: SGL_RS13770 - DUF3082 domain-containing protein, at 2,555,593 to 2,555,916
_RS13770
SGL_RS13775: SGL_RS13775 - hypothetical protein, at 2,556,081 to 2,556,503
_RS13775
Position (kb)
2553
2554
2555
2556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2553.021 kb on - strand at 2553.032 kb on + strand at 2553.711 kb on - strand, within SGL_RS13760 at 2554.023 kb on - strand at 2554.406 kb on + strand, within SGL_RS13765 at 2554.421 kb on + strand, within SGL_RS13765 at 2554.482 kb on - strand, within SGL_RS13765 at 2554.554 kb on - strand, within SGL_RS13765 at 2554.934 kb on - strand, within SGL_RS13765 at 2555.043 kb on - strand, within SGL_RS13765 at 2555.118 kb on - strand, within SGL_RS13765 at 2555.181 kb on - strand, within SGL_RS13765 at 2555.196 kb on - strand, within SGL_RS13765 at 2555.287 kb on + strand, within SGL_RS13765 at 2555.820 kb on - strand, within SGL_RS13770
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,553,021 - -2.1 2,553,032 + -0.4 2,553,711 - SGL_RS13760 0.73 +0.4 2,554,023 - +1.1 2,554,406 + SGL_RS13765 0.30 +0.9 2,554,421 + SGL_RS13765 0.31 +1.7 2,554,482 - SGL_RS13765 0.35 +1.7 2,554,554 - SGL_RS13765 0.40 +1.3 2,554,934 - SGL_RS13765 0.66 +1.8 2,555,043 - SGL_RS13765 0.73 +0.6 2,555,118 - SGL_RS13765 0.78 +1.2 2,555,181 - SGL_RS13765 0.82 +1.1 2,555,196 - SGL_RS13765 0.84 +1.4 2,555,287 + SGL_RS13765 0.90 +2.4 2,555,820 - SGL_RS13770 0.70 +4.5
Or see this region's nucleotide sequence