Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13315 and SGL_RS13320 are separated by 38 nucleotides SGL_RS13320 and SGL_RS13325 are separated by 43 nucleotides SGL_RS13325 and SGL_RS13330 are separated by 166 nucleotides
SGL_RS13315: SGL_RS13315 - hypothetical protein, at 2,454,087 to 2,455,082
_RS13315
SGL_RS13320: SGL_RS13320 - DUF2808 domain-containing protein, at 2,455,121 to 2,455,711
_RS13320
SGL_RS13325: SGL_RS13325 - ABC transporter permease, at 2,455,755 to 2,456,768
_RS13325
SGL_RS13330: SGL_RS13330 - histidine--tRNA ligase, at 2,456,935 to 2,458,278
_RS13330
Position (kb)
2455
2456
2457 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2455.166 kb on + strand at 2455.181 kb on - strand, within SGL_RS13320 at 2455.181 kb on - strand, within SGL_RS13320 at 2455.203 kb on - strand, within SGL_RS13320 at 2455.206 kb on + strand, within SGL_RS13320 at 2455.407 kb on + strand, within SGL_RS13320 at 2455.441 kb on - strand, within SGL_RS13320 at 2455.484 kb on + strand, within SGL_RS13320 at 2455.492 kb on - strand, within SGL_RS13320 at 2455.741 kb on - strand at 2455.855 kb on - strand at 2456.005 kb on + strand, within SGL_RS13325 at 2456.013 kb on - strand, within SGL_RS13325 at 2456.050 kb on + strand, within SGL_RS13325 at 2456.058 kb on - strand, within SGL_RS13325 at 2456.137 kb on + strand, within SGL_RS13325 at 2456.216 kb on + strand, within SGL_RS13325 at 2456.424 kb on + strand, within SGL_RS13325 at 2456.435 kb on + strand, within SGL_RS13325 at 2456.486 kb on - strand, within SGL_RS13325 at 2456.511 kb on + strand, within SGL_RS13325 at 2456.667 kb on + strand at 2456.675 kb on - strand at 2456.675 kb on - strand at 2456.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,455,166 + +0.7 2,455,181 - SGL_RS13320 0.10 +0.5 2,455,181 - SGL_RS13320 0.10 +0.4 2,455,203 - SGL_RS13320 0.14 +0.1 2,455,206 + SGL_RS13320 0.14 +0.4 2,455,407 + SGL_RS13320 0.48 +0.4 2,455,441 - SGL_RS13320 0.54 +0.3 2,455,484 + SGL_RS13320 0.61 +0.2 2,455,492 - SGL_RS13320 0.63 +0.0 2,455,741 - +0.3 2,455,855 - +2.7 2,456,005 + SGL_RS13325 0.25 +1.9 2,456,013 - SGL_RS13325 0.25 +2.6 2,456,050 + SGL_RS13325 0.29 +3.2 2,456,058 - SGL_RS13325 0.30 +1.9 2,456,137 + SGL_RS13325 0.38 +1.9 2,456,216 + SGL_RS13325 0.45 +1.6 2,456,424 + SGL_RS13325 0.66 +1.2 2,456,435 + SGL_RS13325 0.67 +1.3 2,456,486 - SGL_RS13325 0.72 +2.3 2,456,511 + SGL_RS13325 0.75 +1.5 2,456,667 + +2.2 2,456,675 - +3.8 2,456,675 - +1.8 2,456,777 + +1.1
Or see this region's nucleotide sequence