Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13200 and SGL_RS13205 are separated by 130 nucleotides SGL_RS13205 and SGL_RS13210 are separated by 251 nucleotides SGL_RS13210 and SGL_RS13215 are separated by 127 nucleotides SGL_RS13215 and SGL_RS19625 are separated by 36 nucleotides SGL_RS19625 and SGL_RS13220 are separated by 6 nucleotides
SGL_RS13200: SGL_RS13200 - cytochrome b6, at 2,428,010 to 2,428,678
_RS13200
SGL_RS13205: SGL_RS13205 - cytochrome b6-f complex subunit IV, at 2,428,809 to 2,429,291
_RS13205
SGL_RS13210: SGL_RS13210 - glycosyltransferase, at 2,429,543 to 2,430,964
_RS13210
SGL_RS13215: SGL_RS13215 - SxtJ family membrane protein, at 2,431,092 to 2,431,499
_RS13215
SGL_RS19625: SGL_RS19625 - DUF5989 family protein, at 2,431,536 to 2,431,697
_RS19625
SGL_RS13220: SGL_RS13220 - ribonuclease III, at 2,431,704 to 2,432,438
_RS13220
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2428.745 kb on + strand at 2429.275 kb on - strand at 2429.337 kb on - strand at 2429.620 kb on - strand at 2429.702 kb on - strand, within SGL_RS13210 at 2431.189 kb on - strand, within SGL_RS13215 at 2431.285 kb on - strand, within SGL_RS13215 at 2431.803 kb on + strand, within SGL_RS13220 at 2431.816 kb on - strand, within SGL_RS13220 at 2431.875 kb on + strand, within SGL_RS13220 at 2431.909 kb on - strand, within SGL_RS13220 at 2431.924 kb on - strand, within SGL_RS13220
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,428,745 + -2.9 2,429,275 - +2.5 2,429,337 - +0.3 2,429,620 - +0.9 2,429,702 - SGL_RS13210 0.11 +1.0 2,431,189 - SGL_RS13215 0.24 -0.1 2,431,285 - SGL_RS13215 0.47 +1.3 2,431,803 + SGL_RS13220 0.13 +1.0 2,431,816 - SGL_RS13220 0.15 +0.6 2,431,875 + SGL_RS13220 0.23 +1.3 2,431,909 - SGL_RS13220 0.28 -0.1 2,431,924 - SGL_RS13220 0.30 -0.1
Or see this region's nucleotide sequence