Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12685 and SGL_RS12690 overlap by 2 nucleotides SGL_RS12690 and SGL_RS12695 are separated by 227 nucleotides SGL_RS12695 and SGL_RS12700 are separated by 59 nucleotides SGL_RS12700 and SGL_RS12705 overlap by 4 nucleotides
SGL_RS12685: SGL_RS12685 - hypothetical protein, at 2,302,457 to 2,302,639
_RS12685
SGL_RS12690: SGL_RS12690 - hypothetical protein, at 2,302,638 to 2,303,279
_RS12690
SGL_RS12695: SGL_RS12695 - tetratricopeptide repeat protein, at 2,303,507 to 2,304,034
_RS12695
SGL_RS12700: SGL_RS12700 - CBS domain-containing protein, at 2,304,094 to 2,304,561
_RS12700
SGL_RS12705: SGL_RS12705 - recombinase family protein, at 2,304,558 to 2,305,886
_RS12705
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-1
0
1 at 2302.518 kb on + strand, within SGL_RS12685 at 2302.627 kb on - strand at 2302.642 kb on + strand at 2302.678 kb on - strand at 2302.703 kb on - strand, within SGL_RS12690 at 2303.029 kb on + strand, within SGL_RS12690 at 2303.037 kb on - strand, within SGL_RS12690 at 2303.353 kb on + strand at 2303.526 kb on + strand at 2303.881 kb on - strand, within SGL_RS12695 at 2303.944 kb on - strand, within SGL_RS12695 at 2304.270 kb on + strand, within SGL_RS12700 at 2304.857 kb on - strand, within SGL_RS12705 at 2304.935 kb on - strand, within SGL_RS12705 at 2305.000 kb on - strand, within SGL_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,302,518 + SGL_RS12685 0.33 +0.1 2,302,627 - +0.5 2,302,642 + -1.1 2,302,678 - -0.6 2,302,703 - SGL_RS12690 0.10 -0.3 2,303,029 + SGL_RS12690 0.61 -0.3 2,303,037 - SGL_RS12690 0.62 -0.5 2,303,353 + -0.5 2,303,526 + +0.7 2,303,881 - SGL_RS12695 0.71 +1.0 2,303,944 - SGL_RS12695 0.83 +0.8 2,304,270 + SGL_RS12700 0.38 -1.4 2,304,857 - SGL_RS12705 0.22 +0.4 2,304,935 - SGL_RS12705 0.28 +0.3 2,305,000 - SGL_RS12705 0.33 +0.4
Or see this region's nucleotide sequence