Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12660 and SGL_RS12665 are separated by 35 nucleotides SGL_RS12665 and SGL_RS12675 are separated by 252 nucleotides
SGL_RS12660: SGL_RS12660 - DNA double-strand break repair nuclease NurA, at 2,297,368 to 2,298,570
_RS12660
SGL_RS12665: SGL_RS12665 - hypothetical protein, at 2,298,606 to 2,299,118
_RS12665
SGL_RS12675: SGL_RS12675 - Gfo/Idh/MocA family oxidoreductase, at 2,299,371 to 2,300,441
_RS12675
Position (kb)
2298
2299
2300 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2297.635 kb on + strand, within SGL_RS12660 at 2297.635 kb on + strand, within SGL_RS12660 at 2297.643 kb on - strand, within SGL_RS12660 at 2297.672 kb on - strand, within SGL_RS12660 at 2297.737 kb on + strand, within SGL_RS12660 at 2297.885 kb on + strand, within SGL_RS12660 at 2297.885 kb on + strand, within SGL_RS12660 at 2297.893 kb on - strand, within SGL_RS12660 at 2297.972 kb on - strand, within SGL_RS12660 at 2298.047 kb on + strand, within SGL_RS12660 at 2298.054 kb on + strand, within SGL_RS12660 at 2298.087 kb on - strand, within SGL_RS12660 at 2298.103 kb on + strand, within SGL_RS12660 at 2298.172 kb on - strand, within SGL_RS12660 at 2298.205 kb on + strand, within SGL_RS12660 at 2298.213 kb on - strand, within SGL_RS12660 at 2298.264 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.380 kb on + strand, within SGL_RS12660 at 2298.388 kb on - strand, within SGL_RS12660 at 2298.388 kb on - strand, within SGL_RS12660 at 2298.567 kb on + strand at 2298.776 kb on + strand, within SGL_RS12665 at 2298.784 kb on - strand, within SGL_RS12665 at 2298.819 kb on - strand, within SGL_RS12665 at 2298.819 kb on - strand, within SGL_RS12665 at 2298.856 kb on - strand, within SGL_RS12665 at 2298.857 kb on + strand, within SGL_RS12665 at 2298.876 kb on - strand, within SGL_RS12665 at 2298.925 kb on + strand, within SGL_RS12665 at 2299.031 kb on - strand, within SGL_RS12665 at 2299.070 kb on - strand at 2299.103 kb on - strand at 2299.120 kb on + strand at 2299.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,297,635 + SGL_RS12660 0.22 -0.1 2,297,635 + SGL_RS12660 0.22 -0.6 2,297,643 - SGL_RS12660 0.23 -0.1 2,297,672 - SGL_RS12660 0.25 -1.6 2,297,737 + SGL_RS12660 0.31 -3.9 2,297,885 + SGL_RS12660 0.43 -3.7 2,297,885 + SGL_RS12660 0.43 -1.2 2,297,893 - SGL_RS12660 0.44 -2.1 2,297,972 - SGL_RS12660 0.50 -2.5 2,298,047 + SGL_RS12660 0.56 -1.9 2,298,054 + SGL_RS12660 0.57 -2.7 2,298,087 - SGL_RS12660 0.60 -0.1 2,298,103 + SGL_RS12660 0.61 -5.2 2,298,172 - SGL_RS12660 0.67 -1.1 2,298,205 + SGL_RS12660 0.70 -3.1 2,298,213 - SGL_RS12660 0.70 -3.9 2,298,264 + SGL_RS12660 0.74 -0.6 2,298,380 + SGL_RS12660 0.84 -0.6 2,298,380 + SGL_RS12660 0.84 -4.5 2,298,380 + SGL_RS12660 0.84 -3.5 2,298,388 - SGL_RS12660 0.85 -0.1 2,298,388 - SGL_RS12660 0.85 -2.7 2,298,567 + -0.2 2,298,776 + SGL_RS12665 0.33 -3.9 2,298,784 - SGL_RS12665 0.35 -2.8 2,298,819 - SGL_RS12665 0.42 -5.8 2,298,819 - SGL_RS12665 0.42 -5.0 2,298,856 - SGL_RS12665 0.49 -3.7 2,298,857 + SGL_RS12665 0.49 -4.9 2,298,876 - SGL_RS12665 0.53 -5.9 2,298,925 + SGL_RS12665 0.62 -5.0 2,299,031 - SGL_RS12665 0.83 -5.4 2,299,070 - -4.7 2,299,103 - -3.8 2,299,120 + -1.6 2,299,247 - +0.7
Or see this region's nucleotide sequence