Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12545 and SGL_RS12550 are separated by 9 nucleotides SGL_RS12550 and SGL_RS12555 overlap by 1 nucleotides
SGL_RS12545: SGL_RS12545 - GTPase Era, at 2,270,624 to 2,271,571
_RS12545
SGL_RS12550: SGL_RS12550 - hybrid sensor histidine kinase/response regulator, at 2,271,581 to 2,274,868
_RS12550
SGL_RS12555: SGL_RS12555 - alpha-mannosidase, at 2,274,868 to 2,277,996
_RS12555
Position (kb)
2271
2272
2273
2274
2275 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2270.710 kb on - strand at 2271.062 kb on - strand, within SGL_RS12545 at 2271.118 kb on - strand, within SGL_RS12545 at 2271.157 kb on - strand, within SGL_RS12545 at 2271.232 kb on + strand, within SGL_RS12545 at 2271.684 kb on - strand at 2271.751 kb on - strand at 2271.813 kb on - strand at 2272.087 kb on - strand, within SGL_RS12550 at 2272.331 kb on - strand, within SGL_RS12550 at 2272.353 kb on + strand, within SGL_RS12550 at 2272.389 kb on - strand, within SGL_RS12550 at 2272.776 kb on + strand, within SGL_RS12550 at 2272.804 kb on - strand, within SGL_RS12550 at 2272.874 kb on - strand, within SGL_RS12550 at 2273.026 kb on + strand, within SGL_RS12550 at 2273.044 kb on - strand, within SGL_RS12550 at 2273.162 kb on - strand, within SGL_RS12550 at 2273.213 kb on + strand, within SGL_RS12550 at 2273.267 kb on - strand, within SGL_RS12550 at 2273.276 kb on + strand, within SGL_RS12550 at 2273.308 kb on - strand, within SGL_RS12550 at 2273.329 kb on - strand, within SGL_RS12550 at 2273.407 kb on - strand, within SGL_RS12550 at 2273.413 kb on + strand, within SGL_RS12550 at 2273.414 kb on + strand, within SGL_RS12550 at 2273.476 kb on - strand, within SGL_RS12550 at 2273.507 kb on + strand, within SGL_RS12550 at 2273.517 kb on + strand, within SGL_RS12550 at 2273.839 kb on + strand, within SGL_RS12550 at 2273.851 kb on - strand, within SGL_RS12550 at 2273.942 kb on + strand, within SGL_RS12550 at 2273.942 kb on + strand, within SGL_RS12550 at 2273.943 kb on - strand, within SGL_RS12550 at 2274.062 kb on - strand, within SGL_RS12550 at 2274.064 kb on + strand, within SGL_RS12550 at 2274.072 kb on - strand, within SGL_RS12550 at 2274.220 kb on - strand, within SGL_RS12550 at 2274.270 kb on - strand, within SGL_RS12550 at 2274.344 kb on + strand, within SGL_RS12550 at 2274.352 kb on - strand, within SGL_RS12550 at 2274.478 kb on - strand, within SGL_RS12550 at 2274.598 kb on - strand at 2274.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,270,710 - -1.9 2,271,062 - SGL_RS12545 0.46 -2.2 2,271,118 - SGL_RS12545 0.52 -1.1 2,271,157 - SGL_RS12545 0.56 -2.1 2,271,232 + SGL_RS12545 0.64 -1.7 2,271,684 - +2.6 2,271,751 - +2.2 2,271,813 - +0.7 2,272,087 - SGL_RS12550 0.15 +1.9 2,272,331 - SGL_RS12550 0.23 +0.4 2,272,353 + SGL_RS12550 0.23 +2.1 2,272,389 - SGL_RS12550 0.25 +1.9 2,272,776 + SGL_RS12550 0.36 +2.5 2,272,804 - SGL_RS12550 0.37 +1.5 2,272,874 - SGL_RS12550 0.39 +2.0 2,273,026 + SGL_RS12550 0.44 +2.7 2,273,044 - SGL_RS12550 0.44 +2.5 2,273,162 - SGL_RS12550 0.48 +2.5 2,273,213 + SGL_RS12550 0.50 +2.7 2,273,267 - SGL_RS12550 0.51 +2.1 2,273,276 + SGL_RS12550 0.52 +1.7 2,273,308 - SGL_RS12550 0.53 +2.0 2,273,329 - SGL_RS12550 0.53 +2.1 2,273,407 - SGL_RS12550 0.56 +1.7 2,273,413 + SGL_RS12550 0.56 +2.5 2,273,414 + SGL_RS12550 0.56 +2.2 2,273,476 - SGL_RS12550 0.58 +3.6 2,273,507 + SGL_RS12550 0.59 +1.8 2,273,517 + SGL_RS12550 0.59 +2.5 2,273,839 + SGL_RS12550 0.69 +2.9 2,273,851 - SGL_RS12550 0.69 +3.1 2,273,942 + SGL_RS12550 0.72 +2.7 2,273,942 + SGL_RS12550 0.72 +2.3 2,273,943 - SGL_RS12550 0.72 +2.9 2,274,062 - SGL_RS12550 0.75 +2.2 2,274,064 + SGL_RS12550 0.76 +0.6 2,274,072 - SGL_RS12550 0.76 +2.7 2,274,220 - SGL_RS12550 0.80 +2.7 2,274,270 - SGL_RS12550 0.82 +3.2 2,274,344 + SGL_RS12550 0.84 +2.3 2,274,352 - SGL_RS12550 0.84 +0.6 2,274,478 - SGL_RS12550 0.88 -1.0 2,274,598 - +3.4 2,274,598 - +3.7
Or see this region's nucleotide sequence