Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS12460

Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS12450 and SGL_RS12455 are separated by 64 nucleotidesSGL_RS12455 and SGL_RS12460 are separated by 236 nucleotidesSGL_RS12460 and SGL_RS12465 are separated by 160 nucleotides SGL_RS12450: SGL_RS12450 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 2,248,578 to 2,250,176 _RS12450 SGL_RS12455: SGL_RS12455 - preprotein translocase subunit SecG, at 2,250,241 to 2,250,474 _RS12455 SGL_RS12460: SGL_RS12460 - matrixin family metalloprotease, at 2,250,711 to 2,251,496 _RS12460 SGL_RS12465: SGL_RS12465 - dihydrolipoamide acetyltransferase family protein, at 2,251,657 to 2,252,958 _RS12465 Position (kb) 2250 2251 2252Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2250.125 kb on + strandat 2250.145 kb on - strandat 2250.604 kb on - strandat 2250.781 kb on - strandat 2250.949 kb on - strand, within SGL_RS12460at 2251.044 kb on - strand, within SGL_RS12460at 2251.321 kb on + strand, within SGL_RS12460at 2251.352 kb on + strand, within SGL_RS12460at 2251.396 kb on - strand, within SGL_RS12460at 2251.443 kb on - strandat 2251.464 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation
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2,250,125 + +0.1
2,250,145 - -0.6
2,250,604 - +1.2
2,250,781 - -5.4
2,250,949 - SGL_RS12460 0.30 -6.3
2,251,044 - SGL_RS12460 0.42 -4.8
2,251,321 + SGL_RS12460 0.78 -2.2
2,251,352 + SGL_RS12460 0.82 -5.3
2,251,396 - SGL_RS12460 0.87 -5.8
2,251,443 - -2.1
2,251,464 + +0.4

Or see this region's nucleotide sequence