Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11565 and SGL_RS11570 are separated by 230 nucleotides SGL_RS11570 and SGL_RS11575 are separated by 53 nucleotides SGL_RS11575 and SGL_RS11580 are separated by 239 nucleotides SGL_RS11580 and SGL_RS11585 are separated by 128 nucleotides SGL_RS11585 and SGL_RS11590 are separated by 66 nucleotides
SGL_RS11565: SGL_RS11565 - IS4-like element IS4Sa family transposase, at 2,048,497 to 2,049,513
_RS11565
SGL_RS11570: SGL_RS11570 - DUF309 domain-containing protein, at 2,049,744 to 2,050,130
_RS11570
SGL_RS11575: SGL_RS11575 - tRNA-His, at 2,050,184 to 2,050,256
_RS11575
SGL_RS11580: SGL_RS11580 - DUF760 domain-containing protein, at 2,050,496 to 2,050,819
_RS11580
SGL_RS11585: SGL_RS11585 - SsrA-binding protein SmpB, at 2,050,948 to 2,051,412
_RS11585
SGL_RS11590: SGL_RS11590 - hypothetical protein, at 2,051,479 to 2,053,305
_RS11590
Position (kb)
2050
2051 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2049.715 kb on - strand at 2049.765 kb on + strand at 2049.768 kb on - strand at 2049.772 kb on + strand at 2049.808 kb on + strand, within SGL_RS11570 at 2049.815 kb on - strand, within SGL_RS11570 at 2049.891 kb on + strand, within SGL_RS11570 at 2049.903 kb on - strand, within SGL_RS11570 at 2049.904 kb on - strand, within SGL_RS11570 at 2049.993 kb on + strand, within SGL_RS11570 at 2050.053 kb on - strand, within SGL_RS11570 at 2050.104 kb on - strand at 2050.384 kb on - strand at 2050.475 kb on + strand at 2050.543 kb on - strand, within SGL_RS11580 at 2050.555 kb on + strand, within SGL_RS11580 at 2050.756 kb on - strand, within SGL_RS11580 at 2050.837 kb on - strand at 2050.837 kb on - strand at 2051.059 kb on - strand, within SGL_RS11585 at 2051.452 kb on - strand at 2051.670 kb on + strand, within SGL_RS11590 at 2051.810 kb on + strand, within SGL_RS11590
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,049,715 - +0.2 2,049,765 + +0.7 2,049,768 - +1.0 2,049,772 + -0.3 2,049,808 + SGL_RS11570 0.17 +0.8 2,049,815 - SGL_RS11570 0.18 +1.0 2,049,891 + SGL_RS11570 0.38 +1.3 2,049,903 - SGL_RS11570 0.41 +1.4 2,049,904 - SGL_RS11570 0.41 +0.9 2,049,993 + SGL_RS11570 0.64 -1.0 2,050,053 - SGL_RS11570 0.80 +0.0 2,050,104 - +0.4 2,050,384 - +0.0 2,050,475 + +3.0 2,050,543 - SGL_RS11580 0.15 +3.1 2,050,555 + SGL_RS11580 0.18 +3.2 2,050,756 - SGL_RS11580 0.80 +2.8 2,050,837 - +1.8 2,050,837 - +3.4 2,051,059 - SGL_RS11585 0.24 +2.9 2,051,452 - -0.0 2,051,670 + SGL_RS11590 0.10 +0.0 2,051,810 + SGL_RS11590 0.18 -1.9
Or see this region's nucleotide sequence