Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19230 and SGL_RS18925 are separated by 56 nucleotides SGL_RS18925 and SGL_RS11200 are separated by 220 nucleotides SGL_RS11200 and SGL_RS11205 are separated by 290 nucleotides SGL_RS11205 and SGL_RS11210 are separated by 93 nucleotides
SGL_RS19230: SGL_RS19230 - transposase, at 1,972,561 to 1,973,061
_RS19230
SGL_RS18925: SGL_RS18925 - IS1 family transposase, at 1,973,118 to 1,973,390
_RS18925
SGL_RS11200: SGL_RS11200 - alanine--glyoxylate aminotransferase family protein, at 1,973,611 to 1,974,765
_RS11200
SGL_RS11205: SGL_RS11205 - FHA domain-containing protein, at 1,975,056 to 1,975,481
_RS11205
SGL_RS11210: SGL_RS11210 - 2Fe-2S iron-sulfur cluster-binding protein, at 1,975,575 to 1,975,874
_RS11210
Position (kb) 1973
1974
1975 Strain fitness (log2 ratio) -2
-1
0
1 at 1973.275 kb on - strand, within SGL_RS18925 at 1973.297 kb on - strand, within SGL_RS18925 at 1973.414 kb on + strand at 1973.472 kb on - strand at 1973.486 kb on - strand at 1974.896 kb on + strand at 1974.896 kb on + strand at 1974.949 kb on + strand at 1974.992 kb on - strand at 1975.023 kb on - strand at 1975.087 kb on - strand at 1975.104 kb on + strand, within SGL_RS11205 at 1975.112 kb on - strand, within SGL_RS11205 at 1975.311 kb on + strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.394 kb on + strand, within SGL_RS11205 at 1975.455 kb on - strand at 1975.478 kb on - strand at 1975.537 kb on - strand at 1975.572 kb on + strand at 1975.580 kb on - strand at 1975.587 kb on - strand at 1975.713 kb on + strand, within SGL_RS11210
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,973,275 - SGL_RS18925 0.58 +0.5 1,973,297 - SGL_RS18925 0.66 -0.1 1,973,414 + -1.0 1,973,472 - +0.0 1,973,486 - -1.0 1,974,896 + -0.2 1,974,896 + +0.0 1,974,949 + +0.4 1,974,992 - -2.0 1,975,023 - +0.3 1,975,087 - -0.3 1,975,104 + SGL_RS11205 0.11 +0.4 1,975,112 - SGL_RS11205 0.13 +0.9 1,975,311 + SGL_RS11205 0.60 +0.8 1,975,319 - SGL_RS11205 0.62 +0.3 1,975,319 - SGL_RS11205 0.62 -0.6 1,975,319 - SGL_RS11205 0.62 +1.1 1,975,394 + SGL_RS11205 0.79 +0.0 1,975,455 - +0.1 1,975,478 - +0.4 1,975,537 - -0.6 1,975,572 + -0.4 1,975,580 - -0.3 1,975,587 - +0.5 1,975,713 + SGL_RS11210 0.46 +0.3
Or see this region's nucleotide sequence