Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11035 and SGL_RS11040 are separated by 118 nucleotides SGL_RS11040 and SGL_RS11045 are separated by 202 nucleotides SGL_RS11045 and SGL_RS11050 are separated by 26 nucleotides SGL_RS11050 and SGL_RS11055 are separated by 83 nucleotides
SGL_RS11035: SGL_RS11035 - LapA family protein, at 1,937,474 to 1,938,310
_RS11035
SGL_RS11040: SGL_RS11040 - TMEM165/GDT1 family protein, at 1,938,429 to 1,938,782
_RS11040
SGL_RS11045: SGL_RS11045 - glutathione peroxidase, at 1,938,985 to 1,939,494
_RS11045
SGL_RS11050: SGL_RS11050 - DUF3493 domain-containing protein, at 1,939,521 to 1,939,781
_RS11050
SGL_RS11055: SGL_RS11055 - hypothetical protein, at 1,939,865 to 1,940,506
_RS11055
Position (kb)
1938
1939
1940 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1938.415 kb on + strand at 1938.572 kb on + strand, within SGL_RS11040 at 1938.595 kb on - strand, within SGL_RS11040 at 1938.647 kb on - strand, within SGL_RS11040 at 1938.659 kb on + strand, within SGL_RS11040 at 1939.124 kb on + strand, within SGL_RS11045 at 1939.174 kb on - strand, within SGL_RS11045 at 1939.558 kb on - strand, within SGL_RS11050 at 1939.558 kb on - strand, within SGL_RS11050 at 1939.931 kb on + strand, within SGL_RS11055 at 1940.041 kb on + strand, within SGL_RS11055 at 1940.080 kb on - strand, within SGL_RS11055 at 1940.116 kb on - strand, within SGL_RS11055 at 1940.246 kb on - strand, within SGL_RS11055 at 1940.343 kb on + strand, within SGL_RS11055
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,938,415 + +0.1 1,938,572 + SGL_RS11040 0.40 -5.3 1,938,595 - SGL_RS11040 0.47 -2.8 1,938,647 - SGL_RS11040 0.62 -2.6 1,938,659 + SGL_RS11040 0.65 -2.6 1,939,124 + SGL_RS11045 0.27 +0.4 1,939,174 - SGL_RS11045 0.37 +2.3 1,939,558 - SGL_RS11050 0.14 +0.1 1,939,558 - SGL_RS11050 0.14 +0.6 1,939,931 + SGL_RS11055 0.10 +0.1 1,940,041 + SGL_RS11055 0.27 -1.2 1,940,080 - SGL_RS11055 0.33 -0.7 1,940,116 - SGL_RS11055 0.39 -0.3 1,940,246 - SGL_RS11055 0.59 +0.4 1,940,343 + SGL_RS11055 0.74 -0.2
Or see this region's nucleotide sequence