Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11000 and SGL_RS11005 are separated by 42 nucleotides SGL_RS11005 and SGL_RS11010 are separated by 49 nucleotides SGL_RS11010 and SGL_RS11015 overlap by 1 nucleotides SGL_RS11015 and SGL_RS11020 are separated by 246 nucleotides
SGL_RS11000: SGL_RS11000 - sulfate adenylyltransferase, at 1,931,139 to 1,932,311
_RS11000
SGL_RS11005: SGL_RS11005 - glycosyltransferase, at 1,932,354 to 1,933,418
_RS11005
SGL_RS11010: SGL_RS11010 - type II toxin-antitoxin system VapC family toxin, at 1,933,468 to 1,933,854
_RS11010
SGL_RS11015: SGL_RS11015 - DUF2281 domain-containing protein, at 1,933,854 to 1,934,060
_RS11015
SGL_RS11020: SGL_RS11020 - geranylgeranyl reductase, at 1,934,307 to 1,935,530
_RS11020
Position (kb)
1932
1933
1934 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1931.439 kb on - strand, within SGL_RS11000 at 1931.446 kb on + strand, within SGL_RS11000 at 1931.584 kb on + strand, within SGL_RS11000 at 1931.607 kb on - strand, within SGL_RS11000 at 1931.734 kb on + strand, within SGL_RS11000 at 1931.757 kb on + strand, within SGL_RS11000 at 1931.819 kb on - strand, within SGL_RS11000 at 1931.936 kb on + strand, within SGL_RS11000 at 1931.944 kb on - strand, within SGL_RS11000 at 1932.030 kb on + strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.267 kb on + strand at 1932.404 kb on + strand at 1932.716 kb on - strand, within SGL_RS11005 at 1932.744 kb on + strand, within SGL_RS11005 at 1932.752 kb on - strand, within SGL_RS11005 at 1932.771 kb on + strand, within SGL_RS11005 at 1932.816 kb on + strand, within SGL_RS11005 at 1932.823 kb on + strand, within SGL_RS11005 at 1932.868 kb on + strand, within SGL_RS11005 at 1933.055 kb on - strand, within SGL_RS11005 at 1933.122 kb on + strand, within SGL_RS11005 at 1933.224 kb on - strand, within SGL_RS11005 at 1933.327 kb on - strand at 1933.380 kb on + strand at 1933.562 kb on - strand, within SGL_RS11010 at 1933.689 kb on + strand, within SGL_RS11010 at 1934.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,931,439 - SGL_RS11000 0.26 -2.5 1,931,446 + SGL_RS11000 0.26 -2.5 1,931,584 + SGL_RS11000 0.38 -1.2 1,931,607 - SGL_RS11000 0.40 -3.8 1,931,734 + SGL_RS11000 0.51 -1.8 1,931,757 + SGL_RS11000 0.53 -0.8 1,931,819 - SGL_RS11000 0.58 -2.8 1,931,936 + SGL_RS11000 0.68 -0.8 1,931,944 - SGL_RS11000 0.69 -1.8 1,932,030 + SGL_RS11000 0.76 -1.5 1,932,186 - SGL_RS11000 0.89 -1.2 1,932,186 - SGL_RS11000 0.89 -0.2 1,932,267 + -3.1 1,932,404 + +1.8 1,932,716 - SGL_RS11005 0.34 +1.6 1,932,744 + SGL_RS11005 0.37 +2.1 1,932,752 - SGL_RS11005 0.37 +1.5 1,932,771 + SGL_RS11005 0.39 +1.8 1,932,816 + SGL_RS11005 0.43 +1.7 1,932,823 + SGL_RS11005 0.44 +2.1 1,932,868 + SGL_RS11005 0.48 +1.2 1,933,055 - SGL_RS11005 0.66 +1.5 1,933,122 + SGL_RS11005 0.72 +2.0 1,933,224 - SGL_RS11005 0.82 +1.0 1,933,327 - +2.2 1,933,380 + +1.8 1,933,562 - SGL_RS11010 0.24 -1.3 1,933,689 + SGL_RS11010 0.57 -0.5 1,934,174 + -0.2
Or see this region's nucleotide sequence