Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10690 and SGL_RS10695 are separated by 39 nucleotides SGL_RS10695 and SGL_RS10700 are separated by 95 nucleotides SGL_RS10700 and SGL_RS10705 are separated by 8 nucleotides SGL_RS10705 and SGL_RS10710 are separated by 19 nucleotides
SGL_RS10690: SGL_RS10690 - PilN domain-containing protein, at 1,866,843 to 1,867,697
_RS10690
SGL_RS10695: SGL_RS10695 - pilus assembly protein, at 1,867,737 to 1,868,564
_RS10695
SGL_RS10700: SGL_RS10700 - type IV pilus secretin PilQ, at 1,868,660 to 1,871,002
_RS10700
SGL_RS10705: SGL_RS10705 - FkbM family methyltransferase, at 1,871,011 to 1,871,835
_RS10705
SGL_RS10710: SGL_RS10710 - FkbM family methyltransferase, at 1,871,855 to 1,872,589
_RS10710
Position (kb)
1868
1869
1870
1871
1872 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1867.660 kb on - strand at 1867.701 kb on + strand at 1867.749 kb on + strand at 1867.838 kb on - strand, within SGL_RS10695 at 1867.886 kb on + strand, within SGL_RS10695 at 1867.936 kb on + strand, within SGL_RS10695 at 1867.982 kb on - strand, within SGL_RS10695 at 1867.984 kb on + strand, within SGL_RS10695 at 1868.027 kb on + strand, within SGL_RS10695 at 1868.339 kb on - strand, within SGL_RS10695 at 1868.377 kb on + strand, within SGL_RS10695 at 1868.390 kb on - strand, within SGL_RS10695 at 1868.390 kb on - strand, within SGL_RS10695 at 1868.452 kb on + strand, within SGL_RS10695 at 1868.469 kb on + strand, within SGL_RS10695 at 1868.523 kb on + strand at 1868.960 kb on + strand, within SGL_RS10700 at 1869.053 kb on + strand, within SGL_RS10700 at 1869.060 kb on + strand, within SGL_RS10700 at 1869.078 kb on - strand, within SGL_RS10700 at 1869.258 kb on + strand, within SGL_RS10700 at 1869.346 kb on - strand, within SGL_RS10700 at 1869.447 kb on - strand, within SGL_RS10700 at 1869.619 kb on + strand, within SGL_RS10700 at 1869.619 kb on + strand, within SGL_RS10700 at 1869.814 kb on + strand, within SGL_RS10700 at 1870.597 kb on + strand, within SGL_RS10700 at 1871.027 kb on + strand at 1871.299 kb on + strand, within SGL_RS10705 at 1871.358 kb on - strand, within SGL_RS10705 at 1871.939 kb on - strand, within SGL_RS10710 at 1871.941 kb on + strand, within SGL_RS10710 at 1871.986 kb on + strand, within SGL_RS10710 at 1871.996 kb on - strand, within SGL_RS10710
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,867,660 - +3.0 1,867,701 + +1.2 1,867,749 + +2.8 1,867,838 - SGL_RS10695 0.12 +2.7 1,867,886 + SGL_RS10695 0.18 +3.3 1,867,936 + SGL_RS10695 0.24 +3.0 1,867,982 - SGL_RS10695 0.30 +3.3 1,867,984 + SGL_RS10695 0.30 +3.2 1,868,027 + SGL_RS10695 0.35 +3.5 1,868,339 - SGL_RS10695 0.73 +3.7 1,868,377 + SGL_RS10695 0.77 +3.5 1,868,390 - SGL_RS10695 0.79 +2.6 1,868,390 - SGL_RS10695 0.79 +2.6 1,868,452 + SGL_RS10695 0.86 +0.8 1,868,469 + SGL_RS10695 0.88 +0.2 1,868,523 + -1.4 1,868,960 + SGL_RS10700 0.13 +2.2 1,869,053 + SGL_RS10700 0.17 +1.5 1,869,060 + SGL_RS10700 0.17 +1.7 1,869,078 - SGL_RS10700 0.18 +1.0 1,869,258 + SGL_RS10700 0.26 +0.8 1,869,346 - SGL_RS10700 0.29 +2.1 1,869,447 - SGL_RS10700 0.34 +1.0 1,869,619 + SGL_RS10700 0.41 +2.1 1,869,619 + SGL_RS10700 0.41 +0.7 1,869,814 + SGL_RS10700 0.49 +1.0 1,870,597 + SGL_RS10700 0.83 +1.0 1,871,027 + -0.4 1,871,299 + SGL_RS10705 0.35 +0.5 1,871,358 - SGL_RS10705 0.42 +0.2 1,871,939 - SGL_RS10710 0.11 -1.9 1,871,941 + SGL_RS10710 0.12 -0.7 1,871,986 + SGL_RS10710 0.18 -0.5 1,871,996 - SGL_RS10710 0.19 -0.9
Or see this region's nucleotide sequence