Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10685 and SGL_RS10690 are separated by 81 nucleotides SGL_RS10690 and SGL_RS10695 are separated by 39 nucleotides SGL_RS10695 and SGL_RS10700 are separated by 95 nucleotides
SGL_RS10685: SGL_RS10685 - type IV pilus assembly protein PilM, at 1,865,658 to 1,866,761
_RS10685
SGL_RS10690: SGL_RS10690 - PilN domain-containing protein, at 1,866,843 to 1,867,697
_RS10690
SGL_RS10695: SGL_RS10695 - pilus assembly protein, at 1,867,737 to 1,868,564
_RS10695
SGL_RS10700: SGL_RS10700 - type IV pilus secretin PilQ, at 1,868,660 to 1,871,002
_RS10700
Position (kb)
1867
1868
1869 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1866.740 kb on - strand at 1866.740 kb on - strand at 1866.797 kb on - strand at 1866.921 kb on - strand at 1867.009 kb on - strand, within SGL_RS10690 at 1867.068 kb on + strand, within SGL_RS10690 at 1867.218 kb on + strand, within SGL_RS10690 at 1867.481 kb on + strand, within SGL_RS10690 at 1867.660 kb on - strand at 1867.701 kb on + strand at 1867.749 kb on + strand at 1867.838 kb on - strand, within SGL_RS10695 at 1867.886 kb on + strand, within SGL_RS10695 at 1867.936 kb on + strand, within SGL_RS10695 at 1867.982 kb on - strand, within SGL_RS10695 at 1867.984 kb on + strand, within SGL_RS10695 at 1868.027 kb on + strand, within SGL_RS10695 at 1868.339 kb on - strand, within SGL_RS10695 at 1868.377 kb on + strand, within SGL_RS10695 at 1868.390 kb on - strand, within SGL_RS10695 at 1868.390 kb on - strand, within SGL_RS10695 at 1868.452 kb on + strand, within SGL_RS10695 at 1868.469 kb on + strand, within SGL_RS10695 at 1868.523 kb on + strand at 1868.960 kb on + strand, within SGL_RS10700 at 1869.053 kb on + strand, within SGL_RS10700 at 1869.060 kb on + strand, within SGL_RS10700 at 1869.078 kb on - strand, within SGL_RS10700 at 1869.258 kb on + strand, within SGL_RS10700 at 1869.346 kb on - strand, within SGL_RS10700 at 1869.447 kb on - strand, within SGL_RS10700
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,866,740 - +2.8 1,866,740 - +2.2 1,866,797 - +2.6 1,866,921 - +3.6 1,867,009 - SGL_RS10690 0.19 +3.4 1,867,068 + SGL_RS10690 0.26 +2.6 1,867,218 + SGL_RS10690 0.44 +3.5 1,867,481 + SGL_RS10690 0.75 +3.7 1,867,660 - +3.0 1,867,701 + +1.2 1,867,749 + +2.8 1,867,838 - SGL_RS10695 0.12 +2.7 1,867,886 + SGL_RS10695 0.18 +3.3 1,867,936 + SGL_RS10695 0.24 +3.0 1,867,982 - SGL_RS10695 0.30 +3.3 1,867,984 + SGL_RS10695 0.30 +3.2 1,868,027 + SGL_RS10695 0.35 +3.5 1,868,339 - SGL_RS10695 0.73 +3.7 1,868,377 + SGL_RS10695 0.77 +3.5 1,868,390 - SGL_RS10695 0.79 +2.6 1,868,390 - SGL_RS10695 0.79 +2.6 1,868,452 + SGL_RS10695 0.86 +0.8 1,868,469 + SGL_RS10695 0.88 +0.2 1,868,523 + -1.4 1,868,960 + SGL_RS10700 0.13 +2.2 1,869,053 + SGL_RS10700 0.17 +1.5 1,869,060 + SGL_RS10700 0.17 +1.7 1,869,078 - SGL_RS10700 0.18 +1.0 1,869,258 + SGL_RS10700 0.26 +0.8 1,869,346 - SGL_RS10700 0.29 +2.1 1,869,447 - SGL_RS10700 0.34 +1.0
Or see this region's nucleotide sequence