Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS10685

Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS20030 and SGL_RS10680 are separated by 29 nucleotidesSGL_RS10680 and SGL_RS10685 are separated by 184 nucleotidesSGL_RS10685 and SGL_RS10690 are separated by 81 nucleotidesSGL_RS10690 and SGL_RS10695 are separated by 39 nucleotides SGL_RS20030: SGL_RS20030 - iron uptake porin, at 1,864,239 to 1,865,003 _RS20030 SGL_RS10680: SGL_RS10680 - hypothetical protein, at 1,865,033 to 1,865,473 _RS10680 SGL_RS10685: SGL_RS10685 - type IV pilus assembly protein PilM, at 1,865,658 to 1,866,761 _RS10685 SGL_RS10690: SGL_RS10690 - PilN domain-containing protein, at 1,866,843 to 1,867,697 _RS10690 SGL_RS10695: SGL_RS10695 - pilus assembly protein, at 1,867,737 to 1,868,564 _RS10695 Position (kb) 1865 1866 1867Strain fitness (log2 ratio) -1 0 1 2 3at 1864.987 kb on - strandat 1865.053 kb on - strandat 1865.118 kb on + strand, within SGL_RS10680at 1865.409 kb on - strand, within SGL_RS10680at 1865.425 kb on + strand, within SGL_RS10680at 1865.425 kb on + strand, within SGL_RS10680at 1865.433 kb on - strandat 1865.521 kb on + strandat 1865.907 kb on + strand, within SGL_RS10685at 1865.955 kb on + strand, within SGL_RS10685at 1866.008 kb on + strand, within SGL_RS10685at 1866.017 kb on - strand, within SGL_RS10685at 1866.384 kb on + strand, within SGL_RS10685at 1866.442 kb on - strand, within SGL_RS10685at 1866.732 kb on + strandat 1866.740 kb on - strandat 1866.740 kb on - strandat 1866.797 kb on - strandat 1866.921 kb on - strandat 1867.009 kb on - strand, within SGL_RS10690at 1867.068 kb on + strand, within SGL_RS10690at 1867.218 kb on + strand, within SGL_RS10690at 1867.481 kb on + strand, within SGL_RS10690at 1867.660 kb on - strandat 1867.701 kb on + strandat 1867.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation
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1,864,987 - +1.9
1,865,053 - +0.2
1,865,118 + SGL_RS10680 0.19 -0.0
1,865,409 - SGL_RS10680 0.85 +1.1
1,865,425 + SGL_RS10680 0.89 +0.5
1,865,425 + SGL_RS10680 0.89 +0.2
1,865,433 - +1.0
1,865,521 + +1.0
1,865,907 + SGL_RS10685 0.23 +3.5
1,865,955 + SGL_RS10685 0.27 +3.0
1,866,008 + SGL_RS10685 0.32 +3.2
1,866,017 - SGL_RS10685 0.33 +2.7
1,866,384 + SGL_RS10685 0.66 +3.3
1,866,442 - SGL_RS10685 0.71 +3.1
1,866,732 + +3.5
1,866,740 - +2.8
1,866,740 - +2.2
1,866,797 - +2.6
1,866,921 - +3.6
1,867,009 - SGL_RS10690 0.19 +3.4
1,867,068 + SGL_RS10690 0.26 +2.6
1,867,218 + SGL_RS10690 0.44 +3.5
1,867,481 + SGL_RS10690 0.75 +3.7
1,867,660 - +3.0
1,867,701 + +1.2
1,867,749 + +2.8

Or see this region's nucleotide sequence