Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10275 and SGL_RS10280 are separated by 104 nucleotides SGL_RS10280 and SGL_RS10285 are separated by 185 nucleotides SGL_RS10285 and SGL_RS10290 are separated by 203 nucleotides
SGL_RS10275: SGL_RS10275 - ATP-binding protein, at 1,777,729 to 1,779,981
_RS10275
SGL_RS10280: SGL_RS10280 - hypothetical protein, at 1,780,086 to 1,780,298
_RS10280
SGL_RS10285: SGL_RS10285 - late competence development ComFB family protein, at 1,780,484 to 1,781,005
_RS10285
SGL_RS10290: SGL_RS10290 - M48 family metalloprotease, at 1,781,209 to 1,782,060
_RS10290
Position (kb)
1780
1781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1779.420 kb on + strand, within SGL_RS10275 at 1779.462 kb on - strand, within SGL_RS10275 at 1779.855 kb on + strand at 1779.871 kb on - strand at 1779.888 kb on - strand at 1779.964 kb on - strand at 1780.219 kb on + strand, within SGL_RS10280 at 1780.219 kb on + strand, within SGL_RS10280 at 1780.227 kb on - strand, within SGL_RS10280 at 1780.227 kb on - strand, within SGL_RS10280 at 1780.255 kb on - strand, within SGL_RS10280 at 1780.287 kb on - strand at 1780.320 kb on + strand at 1780.383 kb on + strand at 1780.456 kb on + strand at 1780.581 kb on + strand, within SGL_RS10285 at 1780.737 kb on - strand, within SGL_RS10285 at 1780.752 kb on + strand, within SGL_RS10285 at 1780.778 kb on - strand, within SGL_RS10285 at 1780.806 kb on + strand, within SGL_RS10285 at 1780.948 kb on - strand, within SGL_RS10285 at 1780.960 kb on + strand at 1780.978 kb on + strand at 1780.994 kb on + strand at 1780.999 kb on + strand at 1781.046 kb on - strand at 1781.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,779,420 + SGL_RS10275 0.75 -0.2 1,779,462 - SGL_RS10275 0.77 -3.1 1,779,855 + -1.9 1,779,871 - -1.0 1,779,888 - -2.8 1,779,964 - -1.4 1,780,219 + SGL_RS10280 0.62 +3.0 1,780,219 + SGL_RS10280 0.62 +3.1 1,780,227 - SGL_RS10280 0.66 +2.0 1,780,227 - SGL_RS10280 0.66 +2.0 1,780,255 - SGL_RS10280 0.79 +2.6 1,780,287 - +1.0 1,780,320 + -0.1 1,780,383 + -0.6 1,780,456 + +0.0 1,780,581 + SGL_RS10285 0.19 -1.3 1,780,737 - SGL_RS10285 0.48 +0.5 1,780,752 + SGL_RS10285 0.51 +0.1 1,780,778 - SGL_RS10285 0.56 -0.9 1,780,806 + SGL_RS10285 0.62 +0.2 1,780,948 - SGL_RS10285 0.89 -0.2 1,780,960 + -0.2 1,780,978 + -0.1 1,780,994 + +0.0 1,780,999 + +0.0 1,781,046 - -0.7 1,781,053 - -0.2
Or see this region's nucleotide sequence