Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09625 and SGL_RS09630 overlap by 8 nucleotides SGL_RS09630 and SGL_RS09635 are separated by 445 nucleotides SGL_RS09635 and SGL_RS09640 are separated by 38 nucleotides
SGL_RS09625: SGL_RS09625 - hypothetical protein, at 1,649,441 to 1,649,914
_RS09625
SGL_RS09630: SGL_RS09630 - hypothetical protein, at 1,649,907 to 1,650,443
_RS09630
SGL_RS09635: SGL_RS09635 - TldD/PmbA family protein, at 1,650,889 to 1,652,358
_RS09635
SGL_RS09640: SGL_RS09640 - peptide chain release factor N(5)-glutamine methyltransferase, at 1,652,397 to 1,653,296
_RS09640
Position (kb)
1650
1651
1652
1653 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1650.234 kb on - strand, within SGL_RS09630 at 1650.451 kb on - strand at 1650.670 kb on + strand at 1650.706 kb on - strand at 1650.819 kb on + strand at 1650.825 kb on + strand at 1650.825 kb on + strand at 1650.825 kb on + strand at 1650.833 kb on - strand at 1650.848 kb on - strand at 1650.848 kb on - strand at 1650.893 kb on + strand at 1650.893 kb on + strand at 1650.893 kb on + strand at 1650.901 kb on - strand at 1650.930 kb on + strand at 1650.930 kb on + strand at 1650.949 kb on - strand at 1650.987 kb on - strand at 1650.999 kb on + strand at 1651.078 kb on + strand, within SGL_RS09635 at 1651.094 kb on + strand, within SGL_RS09635 at 1651.102 kb on - strand, within SGL_RS09635 at 1651.187 kb on + strand, within SGL_RS09635 at 1651.190 kb on - strand, within SGL_RS09635 at 1651.227 kb on - strand, within SGL_RS09635 at 1651.232 kb on - strand, within SGL_RS09635 at 1651.237 kb on + strand, within SGL_RS09635 at 1651.291 kb on - strand, within SGL_RS09635 at 1651.320 kb on + strand, within SGL_RS09635 at 1651.401 kb on - strand, within SGL_RS09635 at 1651.450 kb on + strand, within SGL_RS09635 at 1651.472 kb on + strand, within SGL_RS09635 at 1651.473 kb on - strand, within SGL_RS09635 at 1651.490 kb on - strand, within SGL_RS09635 at 1651.506 kb on + strand, within SGL_RS09635 at 1651.555 kb on - strand, within SGL_RS09635 at 1651.705 kb on - strand, within SGL_RS09635 at 1651.711 kb on - strand, within SGL_RS09635 at 1651.722 kb on - strand, within SGL_RS09635 at 1651.860 kb on - strand, within SGL_RS09635 at 1651.932 kb on + strand, within SGL_RS09635 at 1651.942 kb on - strand, within SGL_RS09635 at 1651.951 kb on + strand, within SGL_RS09635 at 1651.959 kb on - strand, within SGL_RS09635 at 1651.984 kb on + strand, within SGL_RS09635 at 1651.984 kb on + strand, within SGL_RS09635 at 1652.022 kb on - strand, within SGL_RS09635 at 1652.090 kb on + strand, within SGL_RS09635 at 1652.133 kb on - strand, within SGL_RS09635 at 1652.165 kb on - strand, within SGL_RS09635 at 1652.190 kb on - strand, within SGL_RS09635 at 1652.190 kb on - strand, within SGL_RS09635 at 1652.279 kb on - strand at 1652.296 kb on + strand at 1652.296 kb on + strand at 1652.304 kb on - strand at 1652.306 kb on - strand at 1652.322 kb on + strand at 1652.330 kb on - strand at 1652.330 kb on - strand at 1652.336 kb on + strand at 1652.336 kb on + strand at 1652.420 kb on - strand at 1652.566 kb on + strand, within SGL_RS09640 at 1652.595 kb on - strand, within SGL_RS09640 at 1652.618 kb on - strand, within SGL_RS09640 at 1652.685 kb on - strand, within SGL_RS09640 at 1652.686 kb on + strand, within SGL_RS09640 at 1652.695 kb on + strand, within SGL_RS09640 at 1652.700 kb on + strand, within SGL_RS09640 at 1652.701 kb on + strand, within SGL_RS09640 at 1652.701 kb on + strand, within SGL_RS09640 at 1652.702 kb on - strand, within SGL_RS09640 at 1652.709 kb on - strand, within SGL_RS09640 at 1652.723 kb on + strand, within SGL_RS09640 at 1652.724 kb on + strand, within SGL_RS09640 at 1652.724 kb on + strand, within SGL_RS09640 at 1652.732 kb on - strand, within SGL_RS09640 at 1652.732 kb on - strand, within SGL_RS09640 at 1652.833 kb on + strand, within SGL_RS09640 at 1652.838 kb on + strand, within SGL_RS09640 at 1652.841 kb on - strand, within SGL_RS09640 at 1652.846 kb on - strand, within SGL_RS09640 at 1652.863 kb on + strand, within SGL_RS09640 at 1652.868 kb on + strand, within SGL_RS09640 at 1652.876 kb on - strand, within SGL_RS09640 at 1652.903 kb on - strand, within SGL_RS09640 at 1652.922 kb on + strand, within SGL_RS09640 at 1652.962 kb on - strand, within SGL_RS09640 at 1653.030 kb on + strand, within SGL_RS09640 at 1653.038 kb on - strand, within SGL_RS09640 at 1653.158 kb on + strand, within SGL_RS09640 at 1653.161 kb on - strand, within SGL_RS09640 at 1653.161 kb on - strand, within SGL_RS09640 at 1653.168 kb on + strand, within SGL_RS09640 at 1653.168 kb on + strand, within SGL_RS09640 at 1653.237 kb on + strand at 1653.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,650,234 - SGL_RS09630 0.61 -0.2 1,650,451 - +1.0 1,650,670 + +0.1 1,650,706 - +0.6 1,650,819 + +0.1 1,650,825 + -0.2 1,650,825 + +0.6 1,650,825 + +0.3 1,650,833 - -1.0 1,650,848 - +0.4 1,650,848 - -0.3 1,650,893 + -3.5 1,650,893 + -3.6 1,650,893 + -0.7 1,650,901 - -2.6 1,650,930 + +0.8 1,650,930 + -0.4 1,650,949 - -1.9 1,650,987 - -4.1 1,650,999 + -3.4 1,651,078 + SGL_RS09635 0.13 -1.4 1,651,094 + SGL_RS09635 0.14 -4.2 1,651,102 - SGL_RS09635 0.14 -2.8 1,651,187 + SGL_RS09635 0.20 -4.7 1,651,190 - SGL_RS09635 0.20 -3.4 1,651,227 - SGL_RS09635 0.23 -2.1 1,651,232 - SGL_RS09635 0.23 -1.4 1,651,237 + SGL_RS09635 0.24 -5.3 1,651,291 - SGL_RS09635 0.27 -3.3 1,651,320 + SGL_RS09635 0.29 -3.9 1,651,401 - SGL_RS09635 0.35 -2.2 1,651,450 + SGL_RS09635 0.38 -3.7 1,651,472 + SGL_RS09635 0.40 -3.6 1,651,473 - SGL_RS09635 0.40 -2.0 1,651,490 - SGL_RS09635 0.41 -3.7 1,651,506 + SGL_RS09635 0.42 -3.2 1,651,555 - SGL_RS09635 0.45 -4.5 1,651,705 - SGL_RS09635 0.56 -4.8 1,651,711 - SGL_RS09635 0.56 -4.9 1,651,722 - SGL_RS09635 0.57 -4.2 1,651,860 - SGL_RS09635 0.66 -4.2 1,651,932 + SGL_RS09635 0.71 -2.1 1,651,942 - SGL_RS09635 0.72 -1.6 1,651,951 + SGL_RS09635 0.72 +0.9 1,651,959 - SGL_RS09635 0.73 -5.3 1,651,984 + SGL_RS09635 0.74 -3.8 1,651,984 + SGL_RS09635 0.74 -4.3 1,652,022 - SGL_RS09635 0.77 -3.6 1,652,090 + SGL_RS09635 0.82 -0.1 1,652,133 - SGL_RS09635 0.85 -4.6 1,652,165 - SGL_RS09635 0.87 -4.1 1,652,190 - SGL_RS09635 0.89 -1.8 1,652,190 - SGL_RS09635 0.89 -1.9 1,652,279 - -5.0 1,652,296 + -3.5 1,652,296 + -3.4 1,652,304 - -4.8 1,652,306 - -4.0 1,652,322 + -1.4 1,652,330 - -5.5 1,652,330 - -2.9 1,652,336 + -3.4 1,652,336 + -2.9 1,652,420 - +2.0 1,652,566 + SGL_RS09640 0.19 +2.4 1,652,595 - SGL_RS09640 0.22 +1.6 1,652,618 - SGL_RS09640 0.25 +2.4 1,652,685 - SGL_RS09640 0.32 +1.8 1,652,686 + SGL_RS09640 0.32 +1.9 1,652,695 + SGL_RS09640 0.33 +2.0 1,652,700 + SGL_RS09640 0.34 +2.2 1,652,701 + SGL_RS09640 0.34 +2.4 1,652,701 + SGL_RS09640 0.34 +1.9 1,652,702 - SGL_RS09640 0.34 +2.8 1,652,709 - SGL_RS09640 0.35 +2.6 1,652,723 + SGL_RS09640 0.36 +2.6 1,652,724 + SGL_RS09640 0.36 +2.2 1,652,724 + SGL_RS09640 0.36 +2.6 1,652,732 - SGL_RS09640 0.37 +2.3 1,652,732 - SGL_RS09640 0.37 +1.9 1,652,833 + SGL_RS09640 0.48 +1.5 1,652,838 + SGL_RS09640 0.49 +2.0 1,652,841 - SGL_RS09640 0.49 +2.6 1,652,846 - SGL_RS09640 0.50 +1.0 1,652,863 + SGL_RS09640 0.52 +1.5 1,652,868 + SGL_RS09640 0.52 +1.8 1,652,876 - SGL_RS09640 0.53 +2.2 1,652,903 - SGL_RS09640 0.56 +2.1 1,652,922 + SGL_RS09640 0.58 +2.1 1,652,962 - SGL_RS09640 0.63 +1.7 1,653,030 + SGL_RS09640 0.70 +2.4 1,653,038 - SGL_RS09640 0.71 +1.6 1,653,158 + SGL_RS09640 0.85 +1.5 1,653,161 - SGL_RS09640 0.85 +2.0 1,653,161 - SGL_RS09640 0.85 +2.6 1,653,168 + SGL_RS09640 0.86 +2.9 1,653,168 + SGL_RS09640 0.86 +1.7 1,653,237 + +1.5 1,653,340 + -1.5
Or see this region's nucleotide sequence