Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18840 and SGL_RS09335 are separated by 372 nucleotides SGL_RS09335 and SGL_RS09340 are separated by 85 nucleotides SGL_RS09340 and SGL_RS09345 are separated by 280 nucleotides
SGL_RS18840: SGL_RS18840 - hypothetical protein, at 1,600,044 to 1,600,286
_RS18840
SGL_RS09335: SGL_RS09335 - sodium-dependent bicarbonate transport family permease, at 1,600,659 to 1,601,783
_RS09335
SGL_RS09340: SGL_RS09340 - hypothetical protein, at 1,601,869 to 1,602,201
_RS09340
SGL_RS09345: SGL_RS09345 - NAD(+) kinase, at 1,602,482 to 1,603,405
_RS09345
Position (kb)
1600
1601
1602 Strain fitness (log2 ratio)
-1
0
1
2 at 1599.693 kb on + strand at 1600.197 kb on - strand, within SGL_RS18840 at 1600.706 kb on + strand at 1600.721 kb on - strand at 1600.791 kb on + strand, within SGL_RS09335 at 1600.806 kb on - strand, within SGL_RS09335 at 1600.829 kb on - strand, within SGL_RS09335 at 1600.924 kb on + strand, within SGL_RS09335 at 1600.934 kb on + strand, within SGL_RS09335 at 1600.941 kb on - strand, within SGL_RS09335 at 1600.941 kb on - strand, within SGL_RS09335 at 1600.957 kb on - strand, within SGL_RS09335 at 1601.022 kb on - strand, within SGL_RS09335 at 1601.023 kb on + strand, within SGL_RS09335 at 1601.028 kb on + strand, within SGL_RS09335 at 1601.032 kb on - strand, within SGL_RS09335 at 1601.081 kb on - strand, within SGL_RS09335 at 1601.215 kb on - strand, within SGL_RS09335 at 1601.328 kb on - strand, within SGL_RS09335 at 1601.485 kb on - strand, within SGL_RS09335 at 1601.517 kb on - strand, within SGL_RS09335 at 1601.541 kb on - strand, within SGL_RS09335 at 1601.577 kb on - strand, within SGL_RS09335 at 1601.579 kb on - strand, within SGL_RS09335 at 1601.652 kb on - strand, within SGL_RS09335 at 1601.685 kb on + strand at 1601.760 kb on + strand at 1601.786 kb on - strand at 1601.792 kb on + strand at 1601.804 kb on + strand at 1601.871 kb on + strand at 1601.973 kb on + strand, within SGL_RS09340 at 1602.001 kb on - strand, within SGL_RS09340 at 1602.008 kb on + strand, within SGL_RS09340 at 1602.138 kb on + strand, within SGL_RS09340 at 1602.298 kb on - strand at 1602.684 kb on + strand, within SGL_RS09345 at 1602.697 kb on + strand, within SGL_RS09345 at 1602.734 kb on + strand, within SGL_RS09345
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,599,693 + -0.3 1,600,197 - SGL_RS18840 0.63 -0.2 1,600,706 + +2.1 1,600,721 - +1.6 1,600,791 + SGL_RS09335 0.12 +1.1 1,600,806 - SGL_RS09335 0.13 +1.9 1,600,829 - SGL_RS09335 0.15 +1.6 1,600,924 + SGL_RS09335 0.24 +0.9 1,600,934 + SGL_RS09335 0.24 +1.6 1,600,941 - SGL_RS09335 0.25 +1.6 1,600,941 - SGL_RS09335 0.25 +1.4 1,600,957 - SGL_RS09335 0.26 +1.4 1,601,022 - SGL_RS09335 0.32 +2.0 1,601,023 + SGL_RS09335 0.32 +0.9 1,601,028 + SGL_RS09335 0.33 +1.2 1,601,032 - SGL_RS09335 0.33 +2.0 1,601,081 - SGL_RS09335 0.38 +1.6 1,601,215 - SGL_RS09335 0.49 +1.5 1,601,328 - SGL_RS09335 0.59 +1.4 1,601,485 - SGL_RS09335 0.73 +1.6 1,601,517 - SGL_RS09335 0.76 +2.1 1,601,541 - SGL_RS09335 0.78 +1.7 1,601,577 - SGL_RS09335 0.82 +1.9 1,601,579 - SGL_RS09335 0.82 +1.5 1,601,652 - SGL_RS09335 0.88 +0.7 1,601,685 + +1.5 1,601,760 + +1.7 1,601,786 - -0.7 1,601,792 + -0.0 1,601,804 + -0.3 1,601,871 + -0.6 1,601,973 + SGL_RS09340 0.31 -0.8 1,602,001 - SGL_RS09340 0.40 -0.5 1,602,008 + SGL_RS09340 0.42 -1.5 1,602,138 + SGL_RS09340 0.81 -0.5 1,602,298 - +0.2 1,602,684 + SGL_RS09345 0.22 +0.1 1,602,697 + SGL_RS09345 0.23 -1.2 1,602,734 + SGL_RS09345 0.27 +0.1
Or see this region's nucleotide sequence