Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09255 and SGL_RS09260 are separated by 50 nucleotides SGL_RS09260 and SGL_RS09265 are separated by 113 nucleotides SGL_RS09265 and SGL_RS09270 are separated by 128 nucleotides
SGL_RS09255: SGL_RS09255 - aldo/keto reductase, at 1,582,206 to 1,583,198
_RS09255
SGL_RS09260: SGL_RS09260 - HhoA/HhoB/HtrA family serine endopeptidase, at 1,583,249 to 1,584,499
_RS09260
SGL_RS09265: SGL_RS09265 - hypothetical protein, at 1,584,613 to 1,585,080
_RS09265
SGL_RS09270: SGL_RS09270 - proline--tRNA ligase, at 1,585,209 to 1,587,020
_RS09270
Position (kb)
1583
1584
1585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1582.275 kb on - strand at 1582.448 kb on - strand, within SGL_RS09255 at 1582.551 kb on + strand, within SGL_RS09255 at 1582.598 kb on + strand, within SGL_RS09255 at 1582.624 kb on - strand, within SGL_RS09255 at 1582.678 kb on - strand, within SGL_RS09255 at 1582.713 kb on + strand, within SGL_RS09255 at 1582.775 kb on + strand, within SGL_RS09255 at 1582.824 kb on - strand, within SGL_RS09255 at 1582.862 kb on - strand, within SGL_RS09255 at 1582.899 kb on + strand, within SGL_RS09255 at 1582.969 kb on + strand, within SGL_RS09255 at 1582.969 kb on + strand, within SGL_RS09255 at 1582.969 kb on + strand, within SGL_RS09255 at 1583.016 kb on + strand, within SGL_RS09255 at 1583.024 kb on - strand, within SGL_RS09255 at 1583.154 kb on + strand at 1583.168 kb on - strand at 1583.208 kb on + strand at 1583.288 kb on - strand at 1583.327 kb on + strand at 1583.327 kb on + strand at 1583.335 kb on - strand at 1583.390 kb on - strand, within SGL_RS09260 at 1583.426 kb on + strand, within SGL_RS09260 at 1583.431 kb on + strand, within SGL_RS09260 at 1583.434 kb on - strand, within SGL_RS09260 at 1583.490 kb on + strand, within SGL_RS09260 at 1583.518 kb on - strand, within SGL_RS09260 at 1583.551 kb on + strand, within SGL_RS09260 at 1583.551 kb on + strand, within SGL_RS09260 at 1583.559 kb on - strand, within SGL_RS09260 at 1583.571 kb on + strand, within SGL_RS09260 at 1583.572 kb on + strand, within SGL_RS09260 at 1583.572 kb on + strand, within SGL_RS09260 at 1583.579 kb on - strand, within SGL_RS09260 at 1583.643 kb on + strand, within SGL_RS09260 at 1583.714 kb on - strand, within SGL_RS09260 at 1583.761 kb on - strand, within SGL_RS09260 at 1583.815 kb on - strand, within SGL_RS09260 at 1584.015 kb on - strand, within SGL_RS09260 at 1584.017 kb on + strand, within SGL_RS09260 at 1584.025 kb on - strand, within SGL_RS09260 at 1584.025 kb on - strand, within SGL_RS09260 at 1584.027 kb on + strand, within SGL_RS09260 at 1584.027 kb on + strand, within SGL_RS09260 at 1584.027 kb on + strand, within SGL_RS09260 at 1584.056 kb on + strand, within SGL_RS09260 at 1584.064 kb on - strand, within SGL_RS09260 at 1584.104 kb on - strand, within SGL_RS09260 at 1584.120 kb on + strand, within SGL_RS09260 at 1584.175 kb on - strand, within SGL_RS09260 at 1584.213 kb on + strand, within SGL_RS09260 at 1584.236 kb on + strand, within SGL_RS09260 at 1584.280 kb on + strand, within SGL_RS09260 at 1584.359 kb on + strand, within SGL_RS09260 at 1584.359 kb on + strand, within SGL_RS09260 at 1584.367 kb on - strand, within SGL_RS09260 at 1584.367 kb on - strand, within SGL_RS09260 at 1584.367 kb on - strand, within SGL_RS09260 at 1584.367 kb on - strand, within SGL_RS09260 at 1584.457 kb on - strand at 1584.685 kb on + strand, within SGL_RS09265 at 1584.777 kb on + strand, within SGL_RS09265 at 1584.971 kb on + strand, within SGL_RS09265 at 1584.979 kb on - strand, within SGL_RS09265 at 1585.017 kb on - strand, within SGL_RS09265 at 1585.066 kb on - strand at 1585.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,582,275 - +1.3 1,582,448 - SGL_RS09255 0.24 +0.5 1,582,551 + SGL_RS09255 0.35 -0.4 1,582,598 + SGL_RS09255 0.39 +0.5 1,582,624 - SGL_RS09255 0.42 +0.1 1,582,678 - SGL_RS09255 0.48 -0.8 1,582,713 + SGL_RS09255 0.51 +0.2 1,582,775 + SGL_RS09255 0.57 +0.3 1,582,824 - SGL_RS09255 0.62 +0.4 1,582,862 - SGL_RS09255 0.66 +0.4 1,582,899 + SGL_RS09255 0.70 +0.4 1,582,969 + SGL_RS09255 0.77 +0.2 1,582,969 + SGL_RS09255 0.77 -1.3 1,582,969 + SGL_RS09255 0.77 +0.3 1,583,016 + SGL_RS09255 0.82 +0.2 1,583,024 - SGL_RS09255 0.82 +0.5 1,583,154 + +0.3 1,583,168 - +0.2 1,583,208 + -1.7 1,583,288 - +0.5 1,583,327 + +1.3 1,583,327 + +2.6 1,583,335 - +1.0 1,583,390 - SGL_RS09260 0.11 +0.4 1,583,426 + SGL_RS09260 0.14 +1.0 1,583,431 + SGL_RS09260 0.15 +2.1 1,583,434 - SGL_RS09260 0.15 +1.8 1,583,490 + SGL_RS09260 0.19 -0.1 1,583,518 - SGL_RS09260 0.22 +1.4 1,583,551 + SGL_RS09260 0.24 +0.2 1,583,551 + SGL_RS09260 0.24 +1.6 1,583,559 - SGL_RS09260 0.25 +0.0 1,583,571 + SGL_RS09260 0.26 +0.9 1,583,572 + SGL_RS09260 0.26 +1.2 1,583,572 + SGL_RS09260 0.26 +0.2 1,583,579 - SGL_RS09260 0.26 +1.6 1,583,643 + SGL_RS09260 0.31 +0.8 1,583,714 - SGL_RS09260 0.37 +0.9 1,583,761 - SGL_RS09260 0.41 +1.2 1,583,815 - SGL_RS09260 0.45 +1.0 1,584,015 - SGL_RS09260 0.61 +1.3 1,584,017 + SGL_RS09260 0.61 +1.0 1,584,025 - SGL_RS09260 0.62 +1.1 1,584,025 - SGL_RS09260 0.62 +0.4 1,584,027 + SGL_RS09260 0.62 +1.0 1,584,027 + SGL_RS09260 0.62 +1.6 1,584,027 + SGL_RS09260 0.62 +0.5 1,584,056 + SGL_RS09260 0.65 +2.6 1,584,064 - SGL_RS09260 0.65 +1.0 1,584,104 - SGL_RS09260 0.68 +1.0 1,584,120 + SGL_RS09260 0.70 +1.1 1,584,175 - SGL_RS09260 0.74 +1.1 1,584,213 + SGL_RS09260 0.77 +1.7 1,584,236 + SGL_RS09260 0.79 -0.3 1,584,280 + SGL_RS09260 0.82 -0.0 1,584,359 + SGL_RS09260 0.89 +0.9 1,584,359 + SGL_RS09260 0.89 +1.0 1,584,367 - SGL_RS09260 0.89 +1.0 1,584,367 - SGL_RS09260 0.89 -0.3 1,584,367 - SGL_RS09260 0.89 +0.6 1,584,367 - SGL_RS09260 0.89 +0.8 1,584,457 - +0.8 1,584,685 + SGL_RS09265 0.15 -3.5 1,584,777 + SGL_RS09265 0.35 -0.7 1,584,971 + SGL_RS09265 0.76 +0.4 1,584,979 - SGL_RS09265 0.78 +0.3 1,585,017 - SGL_RS09265 0.86 +0.2 1,585,066 - -1.1 1,585,206 + -0.1
Or see this region's nucleotide sequence