Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08605 and SGL_RS08610 are separated by 21 nucleotides SGL_RS08610 and SGL_RS08615 are separated by 40 nucleotides SGL_RS08615 and SGL_RS08620 are separated by 26 nucleotides
SGL_RS08605: SGL_RS08605 - DUF3120 domain-containing protein, at 1,434,901 to 1,435,623
_RS08605
SGL_RS08610: SGL_RS08610 - adenylate/guanylate cyclase domain-containing protein, at 1,435,645 to 1,436,658
_RS08610
SGL_RS08615: SGL_RS08615 - glutathione peroxidase, at 1,436,699 to 1,437,163
_RS08615
SGL_RS08620: SGL_RS08620 - thiolase family protein, at 1,437,190 to 1,438,602
_RS08620
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1434.722 kb on + strand at 1434.722 kb on + strand at 1434.745 kb on - strand at 1434.745 kb on - strand at 1434.890 kb on - strand at 1434.971 kb on - strand at 1435.146 kb on + strand, within SGL_RS08605 at 1435.245 kb on - strand, within SGL_RS08605 at 1435.257 kb on + strand, within SGL_RS08605 at 1435.264 kb on + strand, within SGL_RS08605 at 1435.333 kb on - strand, within SGL_RS08605 at 1435.444 kb on - strand, within SGL_RS08605 at 1435.497 kb on - strand, within SGL_RS08605 at 1435.808 kb on + strand, within SGL_RS08610 at 1435.941 kb on + strand, within SGL_RS08610 at 1435.949 kb on - strand, within SGL_RS08610 at 1436.072 kb on - strand, within SGL_RS08610 at 1436.077 kb on + strand, within SGL_RS08610 at 1436.085 kb on - strand, within SGL_RS08610 at 1436.146 kb on - strand, within SGL_RS08610 at 1436.305 kb on + strand, within SGL_RS08610 at 1436.384 kb on + strand, within SGL_RS08610 at 1436.502 kb on - strand, within SGL_RS08610 at 1436.550 kb on + strand, within SGL_RS08610 at 1436.558 kb on - strand at 1436.601 kb on - strand at 1436.619 kb on - strand at 1436.629 kb on - strand at 1436.651 kb on + strand at 1436.732 kb on - strand at 1436.751 kb on - strand, within SGL_RS08615 at 1436.818 kb on - strand, within SGL_RS08615 at 1436.831 kb on + strand, within SGL_RS08615 at 1436.831 kb on - strand, within SGL_RS08615 at 1436.836 kb on + strand, within SGL_RS08615 at 1436.868 kb on + strand, within SGL_RS08615 at 1436.998 kb on - strand, within SGL_RS08615 at 1437.197 kb on + strand at 1437.389 kb on + strand, within SGL_RS08620 at 1437.448 kb on + strand, within SGL_RS08620 at 1437.450 kb on + strand, within SGL_RS08620 at 1437.551 kb on + strand, within SGL_RS08620 at 1437.551 kb on + strand, within SGL_RS08620 at 1437.584 kb on + strand, within SGL_RS08620 at 1437.619 kb on + strand, within SGL_RS08620
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,434,722 + +1.9 1,434,722 + +1.9 1,434,745 - +0.3 1,434,745 - +0.9 1,434,890 - +2.1 1,434,971 - +2.3 1,435,146 + SGL_RS08605 0.34 -0.7 1,435,245 - SGL_RS08605 0.48 -0.6 1,435,257 + SGL_RS08605 0.49 -0.6 1,435,264 + SGL_RS08605 0.50 -1.7 1,435,333 - SGL_RS08605 0.60 -0.5 1,435,444 - SGL_RS08605 0.75 -0.5 1,435,497 - SGL_RS08605 0.82 -0.4 1,435,808 + SGL_RS08610 0.16 +2.8 1,435,941 + SGL_RS08610 0.29 +3.0 1,435,949 - SGL_RS08610 0.30 +3.3 1,436,072 - SGL_RS08610 0.42 +3.2 1,436,077 + SGL_RS08610 0.43 +3.2 1,436,085 - SGL_RS08610 0.43 +3.2 1,436,146 - SGL_RS08610 0.49 +2.9 1,436,305 + SGL_RS08610 0.65 +2.8 1,436,384 + SGL_RS08610 0.73 +1.1 1,436,502 - SGL_RS08610 0.85 +2.1 1,436,550 + SGL_RS08610 0.89 +3.4 1,436,558 - +2.5 1,436,601 - +2.2 1,436,619 - +2.5 1,436,629 - +2.7 1,436,651 + +0.6 1,436,732 - +0.0 1,436,751 - SGL_RS08615 0.11 -0.5 1,436,818 - SGL_RS08615 0.26 -0.8 1,436,831 + SGL_RS08615 0.28 +0.3 1,436,831 - SGL_RS08615 0.28 +0.1 1,436,836 + SGL_RS08615 0.29 +0.2 1,436,868 + SGL_RS08615 0.36 +1.1 1,436,998 - SGL_RS08615 0.64 -1.1 1,437,197 + -0.5 1,437,389 + SGL_RS08620 0.14 +0.2 1,437,448 + SGL_RS08620 0.18 -0.4 1,437,450 + SGL_RS08620 0.18 -0.1 1,437,551 + SGL_RS08620 0.26 +0.2 1,437,551 + SGL_RS08620 0.26 +1.0 1,437,584 + SGL_RS08620 0.28 +0.7 1,437,619 + SGL_RS08620 0.30 +0.7
Or see this region's nucleotide sequence