Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08405 and SGL_RS08410 are separated by 180 nucleotides SGL_RS08410 and SGL_RS08415 are separated by 128 nucleotides SGL_RS08415 and SGL_RS08420 are separated by 31 nucleotides
SGL_RS08405: SGL_RS08405 - phosphate ABC transporter ATP-binding protein PstB, at 1,391,976 to 1,392,791
_RS08405
SGL_RS08410: SGL_RS08410 - peptidylprolyl isomerase, at 1,392,972 to 1,393,487
_RS08410
SGL_RS08415: SGL_RS08415 - 4a-hydroxytetrahydrobiopterin dehydratase, at 1,393,616 to 1,393,906
_RS08415
SGL_RS08420: SGL_RS08420 - DUF389 domain-containing protein, at 1,393,938 to 1,395,755
_RS08420
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-1
0
1 at 1392.028 kb on - strand at 1392.045 kb on - strand at 1392.045 kb on - strand at 1392.075 kb on + strand, within SGL_RS08405 at 1392.108 kb on + strand, within SGL_RS08405 at 1392.422 kb on - strand, within SGL_RS08405 at 1392.453 kb on + strand, within SGL_RS08405 at 1392.522 kb on - strand, within SGL_RS08405 at 1392.646 kb on + strand, within SGL_RS08405 at 1392.660 kb on + strand, within SGL_RS08405 at 1392.878 kb on + strand at 1392.893 kb on + strand at 1393.181 kb on - strand, within SGL_RS08410 at 1393.309 kb on - strand, within SGL_RS08410 at 1393.379 kb on - strand, within SGL_RS08410 at 1393.693 kb on + strand, within SGL_RS08415 at 1393.822 kb on + strand, within SGL_RS08415 at 1393.892 kb on - strand at 1394.112 kb on + strand at 1394.181 kb on + strand, within SGL_RS08420 at 1394.189 kb on - strand, within SGL_RS08420 at 1394.323 kb on - strand, within SGL_RS08420 at 1394.421 kb on + strand, within SGL_RS08420
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,392,028 - +0.6 1,392,045 - +0.3 1,392,045 - -0.2 1,392,075 + SGL_RS08405 0.12 -1.1 1,392,108 + SGL_RS08405 0.16 -0.4 1,392,422 - SGL_RS08405 0.55 +0.4 1,392,453 + SGL_RS08405 0.58 -0.3 1,392,522 - SGL_RS08405 0.67 +0.7 1,392,646 + SGL_RS08405 0.82 +0.8 1,392,660 + SGL_RS08405 0.84 +0.3 1,392,878 + -0.2 1,392,893 + +0.2 1,393,181 - SGL_RS08410 0.41 +0.4 1,393,309 - SGL_RS08410 0.65 +1.1 1,393,379 - SGL_RS08410 0.79 +1.4 1,393,693 + SGL_RS08415 0.26 +0.5 1,393,822 + SGL_RS08415 0.71 -0.1 1,393,892 - +0.8 1,394,112 + -0.3 1,394,181 + SGL_RS08420 0.13 -0.6 1,394,189 - SGL_RS08420 0.14 +0.1 1,394,323 - SGL_RS08420 0.21 +0.3 1,394,421 + SGL_RS08420 0.27 -1.1
Or see this region's nucleotide sequence