Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07880 and SGL_RS07885 are separated by 161 nucleotides SGL_RS07885 and SGL_RS07890 overlap by 35 nucleotides SGL_RS07890 and SGL_RS07895 overlap by 32 nucleotides SGL_RS07895 and SGL_RS20140 are separated by 29 nucleotides SGL_RS20140 and SGL_RS07900 are separated by 115 nucleotides
SGL_RS07880: SGL_RS07880 - glutamyl-tRNA reductase, at 1,284,660 to 1,285,943
_RS07880
SGL_RS07885: SGL_RS07885 - hypothetical protein, at 1,286,105 to 1,286,902
_RS07885
SGL_RS07890: SGL_RS07890 - ABC transporter substrate-binding protein, at 1,286,868 to 1,288,763
_RS07890
SGL_RS07895: SGL_RS07895 - hypothetical protein, at 1,288,732 to 1,289,112
_RS07895
SGL_RS20140: SGL_RS20140 - hypothetical protein, at 1,289,142 to 1,289,273
_RS20140
SGL_RS07900: SGL_RS07900 - DUF362 domain-containing protein, at 1,289,389 to 1,290,354
_RS07900
Position (kb) 1286
1287
1288
1289 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1286.095 kb on + strand at 1286.095 kb on + strand at 1286.103 kb on - strand at 1286.109 kb on + strand at 1286.156 kb on + strand at 1286.164 kb on - strand at 1286.195 kb on + strand, within SGL_RS07885 at 1286.247 kb on - strand, within SGL_RS07885 at 1286.289 kb on - strand, within SGL_RS07885 at 1286.461 kb on + strand, within SGL_RS07885 at 1286.615 kb on + strand, within SGL_RS07885 at 1286.756 kb on - strand, within SGL_RS07885 at 1286.911 kb on + strand at 1287.056 kb on - strand at 1287.147 kb on + strand, within SGL_RS07890 at 1287.160 kb on - strand, within SGL_RS07890 at 1287.160 kb on - strand, within SGL_RS07890 at 1287.167 kb on + strand, within SGL_RS07890 at 1287.176 kb on + strand, within SGL_RS07890 at 1287.185 kb on - strand, within SGL_RS07890 at 1287.203 kb on - strand, within SGL_RS07890 at 1287.840 kb on + strand, within SGL_RS07890 at 1287.906 kb on + strand, within SGL_RS07890 at 1288.166 kb on - strand, within SGL_RS07890 at 1288.355 kb on + strand, within SGL_RS07890 at 1288.382 kb on + strand, within SGL_RS07890 at 1288.390 kb on - strand, within SGL_RS07890 at 1288.488 kb on - strand, within SGL_RS07890 at 1288.782 kb on + strand, within SGL_RS07895 at 1288.949 kb on + strand, within SGL_RS07895 at 1289.017 kb on + strand, within SGL_RS07895 at 1289.025 kb on - strand, within SGL_RS07895 at 1289.050 kb on - strand, within SGL_RS07895 at 1289.116 kb on - strand at 1289.223 kb on + strand, within SGL_RS20140 at 1289.223 kb on + strand, within SGL_RS20140 at 1289.231 kb on - strand, within SGL_RS20140 at 1289.473 kb on - strand at 1289.473 kb on - strand at 1289.473 kb on - strand at 1289.538 kb on - strand, within SGL_RS07900 at 1289.556 kb on - strand, within SGL_RS07900 at 1289.588 kb on - strand, within SGL_RS07900 at 1289.600 kb on + strand, within SGL_RS07900 at 1289.743 kb on - strand, within SGL_RS07900
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,286,095 + -0.1 1,286,095 + -1.8 1,286,103 - -1.1 1,286,109 + -2.0 1,286,156 + -0.1 1,286,164 - -0.8 1,286,195 + SGL_RS07885 0.11 -0.5 1,286,247 - SGL_RS07885 0.18 -0.4 1,286,289 - SGL_RS07885 0.23 -0.6 1,286,461 + SGL_RS07885 0.45 -0.1 1,286,615 + SGL_RS07885 0.64 -1.3 1,286,756 - SGL_RS07885 0.82 -0.2 1,286,911 + +1.3 1,287,056 - +1.5 1,287,147 + SGL_RS07890 0.15 +1.7 1,287,160 - SGL_RS07890 0.15 +2.3 1,287,160 - SGL_RS07890 0.15 +2.3 1,287,167 + SGL_RS07890 0.16 +1.5 1,287,176 + SGL_RS07890 0.16 +1.9 1,287,185 - SGL_RS07890 0.17 +1.5 1,287,203 - SGL_RS07890 0.18 +2.3 1,287,840 + SGL_RS07890 0.51 +2.0 1,287,906 + SGL_RS07890 0.55 +1.4 1,288,166 - SGL_RS07890 0.68 +2.1 1,288,355 + SGL_RS07890 0.78 +1.9 1,288,382 + SGL_RS07890 0.80 +2.5 1,288,390 - SGL_RS07890 0.80 +2.9 1,288,488 - SGL_RS07890 0.85 +2.5 1,288,782 + SGL_RS07895 0.13 +0.9 1,288,949 + SGL_RS07895 0.57 +2.2 1,289,017 + SGL_RS07895 0.75 +1.6 1,289,025 - SGL_RS07895 0.77 +1.5 1,289,050 - SGL_RS07895 0.83 +0.4 1,289,116 - -0.6 1,289,223 + SGL_RS20140 0.61 +0.3 1,289,223 + SGL_RS20140 0.61 -0.5 1,289,231 - SGL_RS20140 0.67 +0.0 1,289,473 - +1.8 1,289,473 - +0.1 1,289,473 - +0.3 1,289,538 - SGL_RS07900 0.15 -0.9 1,289,556 - SGL_RS07900 0.17 +0.4 1,289,588 - SGL_RS07900 0.21 -0.7 1,289,600 + SGL_RS07900 0.22 +0.4 1,289,743 - SGL_RS07900 0.37 +0.4
Or see this region's nucleotide sequence