Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07845 and SGL_RS07850 are separated by 49 nucleotides SGL_RS07850 and SGL_RS07855 are separated by 251 nucleotides SGL_RS07855 and SGL_RS07860 are separated by 24 nucleotides SGL_RS07860 and SGL_RS07865 are separated by 23 nucleotides
SGL_RS07845: SGL_RS07845 - DUF427 domain-containing protein, at 1,277,108 to 1,277,392
_RS07845
SGL_RS07850: SGL_RS07850 - response regulator transcription factor, at 1,277,442 to 1,278,074
_RS07850
SGL_RS07855: SGL_RS07855 - MASE1 domain-containing protein, at 1,278,326 to 1,280,542
_RS07855
SGL_RS07860: SGL_RS07860 - SDR family NAD(P)-dependent oxidoreductase, at 1,280,567 to 1,281,337
_RS07860
SGL_RS07865: SGL_RS07865 - signal peptide peptidase SppA, at 1,281,361 to 1,283,193
_RS07865
Position (kb)
1278
1279
1280
1281 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1277.345 kb on - strand, within SGL_RS07845 at 1277.401 kb on - strand at 1277.402 kb on - strand at 1277.421 kb on + strand at 1277.422 kb on + strand at 1277.710 kb on - strand, within SGL_RS07850 at 1277.721 kb on - strand, within SGL_RS07850 at 1277.750 kb on + strand, within SGL_RS07850 at 1277.759 kb on - strand, within SGL_RS07850 at 1277.801 kb on - strand, within SGL_RS07850 at 1277.930 kb on + strand, within SGL_RS07850 at 1278.020 kb on - strand at 1278.348 kb on - strand at 1278.398 kb on + strand at 1278.399 kb on + strand at 1278.407 kb on - strand at 1278.407 kb on - strand at 1278.414 kb on - strand at 1278.416 kb on + strand at 1278.416 kb on + strand at 1278.416 kb on + strand at 1278.416 kb on + strand at 1278.424 kb on - strand at 1278.477 kb on + strand at 1278.519 kb on + strand at 1278.552 kb on - strand, within SGL_RS07855 at 1278.611 kb on - strand, within SGL_RS07855 at 1278.618 kb on - strand, within SGL_RS07855 at 1278.782 kb on + strand, within SGL_RS07855 at 1278.792 kb on + strand, within SGL_RS07855 at 1278.865 kb on + strand, within SGL_RS07855 at 1278.987 kb on + strand, within SGL_RS07855 at 1279.123 kb on - strand, within SGL_RS07855 at 1279.123 kb on - strand, within SGL_RS07855 at 1279.233 kb on + strand, within SGL_RS07855 at 1279.380 kb on - strand, within SGL_RS07855 at 1279.422 kb on + strand, within SGL_RS07855 at 1279.561 kb on + strand, within SGL_RS07855 at 1279.574 kb on - strand, within SGL_RS07855 at 1279.634 kb on + strand, within SGL_RS07855 at 1279.656 kb on + strand, within SGL_RS07855 at 1279.967 kb on + strand, within SGL_RS07855 at 1279.968 kb on + strand, within SGL_RS07855 at 1279.975 kb on - strand, within SGL_RS07855 at 1279.988 kb on + strand, within SGL_RS07855 at 1279.988 kb on + strand, within SGL_RS07855 at 1279.988 kb on + strand, within SGL_RS07855 at 1279.996 kb on - strand, within SGL_RS07855 at 1279.996 kb on - strand, within SGL_RS07855 at 1279.996 kb on - strand, within SGL_RS07855 at 1280.040 kb on + strand, within SGL_RS07855 at 1280.231 kb on + strand, within SGL_RS07855 at 1280.243 kb on + strand, within SGL_RS07855 at 1280.322 kb on + strand at 1280.354 kb on + strand at 1280.355 kb on - strand at 1280.396 kb on - strand at 1280.426 kb on + strand at 1280.436 kb on + strand at 1280.527 kb on + strand at 1280.587 kb on + strand at 1280.706 kb on + strand, within SGL_RS07860 at 1280.860 kb on + strand, within SGL_RS07860 at 1280.868 kb on - strand, within SGL_RS07860 at 1280.995 kb on + strand, within SGL_RS07860 at 1281.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,277,345 - SGL_RS07845 0.83 -1.2 1,277,401 - +0.1 1,277,402 - -3.9 1,277,421 + +1.0 1,277,422 + +1.8 1,277,710 - SGL_RS07850 0.42 -0.9 1,277,721 - SGL_RS07850 0.44 -0.4 1,277,750 + SGL_RS07850 0.49 -0.1 1,277,759 - SGL_RS07850 0.50 -0.0 1,277,801 - SGL_RS07850 0.57 -2.0 1,277,930 + SGL_RS07850 0.77 -0.3 1,278,020 - -0.3 1,278,348 - -0.9 1,278,398 + -3.3 1,278,399 + -1.3 1,278,407 - -2.1 1,278,407 - -2.3 1,278,414 - -2.7 1,278,416 + -1.3 1,278,416 + -2.3 1,278,416 + -0.8 1,278,416 + -1.0 1,278,424 - -1.2 1,278,477 + -1.5 1,278,519 + -2.8 1,278,552 - SGL_RS07855 0.10 -2.0 1,278,611 - SGL_RS07855 0.13 -3.8 1,278,618 - SGL_RS07855 0.13 -2.0 1,278,782 + SGL_RS07855 0.21 -4.7 1,278,792 + SGL_RS07855 0.21 -1.2 1,278,865 + SGL_RS07855 0.24 -5.4 1,278,987 + SGL_RS07855 0.30 -3.1 1,279,123 - SGL_RS07855 0.36 -3.7 1,279,123 - SGL_RS07855 0.36 -4.7 1,279,233 + SGL_RS07855 0.41 -2.9 1,279,380 - SGL_RS07855 0.48 -4.4 1,279,422 + SGL_RS07855 0.49 -4.5 1,279,561 + SGL_RS07855 0.56 -3.3 1,279,574 - SGL_RS07855 0.56 -4.1 1,279,634 + SGL_RS07855 0.59 -2.5 1,279,656 + SGL_RS07855 0.60 -4.3 1,279,967 + SGL_RS07855 0.74 -0.5 1,279,968 + SGL_RS07855 0.74 -3.4 1,279,975 - SGL_RS07855 0.74 -4.1 1,279,988 + SGL_RS07855 0.75 -1.8 1,279,988 + SGL_RS07855 0.75 -5.0 1,279,988 + SGL_RS07855 0.75 -3.5 1,279,996 - SGL_RS07855 0.75 -5.1 1,279,996 - SGL_RS07855 0.75 -3.0 1,279,996 - SGL_RS07855 0.75 -5.7 1,280,040 + SGL_RS07855 0.77 -1.5 1,280,231 + SGL_RS07855 0.86 -2.4 1,280,243 + SGL_RS07855 0.86 -3.2 1,280,322 + -3.1 1,280,354 + -2.2 1,280,355 - -3.3 1,280,396 - -3.5 1,280,426 + -5.1 1,280,436 + -4.4 1,280,527 + -1.4 1,280,587 + -0.6 1,280,706 + SGL_RS07860 0.18 +0.5 1,280,860 + SGL_RS07860 0.38 +0.1 1,280,868 - SGL_RS07860 0.39 -0.0 1,280,995 + SGL_RS07860 0.56 -0.3 1,281,502 - -0.9
Or see this region's nucleotide sequence