Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19585 and SGL_RS07695 are separated by 125 nucleotides SGL_RS07695 and SGL_RS07700 are separated by 85 nucleotides SGL_RS07700 and SGL_RS07705 are separated by 32 nucleotides
SGL_RS19585: SGL_RS19585 - hypothetical protein, at 1,244,438 to 1,244,605
_RS19585
SGL_RS07695: SGL_RS07695 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK, at 1,244,731 to 1,245,309
_RS07695
SGL_RS07700: SGL_RS07700 - ribosome silencing factor, at 1,245,395 to 1,245,859
_RS07700
SGL_RS07705: SGL_RS07705 - hypothetical protein, at 1,245,892 to 1,247,052
_RS07705
Position (kb)
1245
1246 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1244.414 kb on - strand at 1244.552 kb on + strand, within SGL_RS19585 at 1244.621 kb on + strand at 1244.628 kb on - strand at 1244.835 kb on - strand, within SGL_RS07695 at 1244.882 kb on - strand, within SGL_RS07695 at 1244.989 kb on - strand, within SGL_RS07695 at 1245.080 kb on - strand, within SGL_RS07695 at 1245.094 kb on + strand, within SGL_RS07695 at 1245.125 kb on + strand, within SGL_RS07695 at 1245.141 kb on + strand, within SGL_RS07695 at 1245.148 kb on + strand, within SGL_RS07695 at 1245.207 kb on + strand, within SGL_RS07695 at 1245.339 kb on + strand at 1245.730 kb on - strand, within SGL_RS07700 at 1245.854 kb on + strand at 1245.861 kb on - strand at 1246.125 kb on + strand, within SGL_RS07705 at 1246.326 kb on - strand, within SGL_RS07705 at 1246.336 kb on + strand, within SGL_RS07705 at 1246.381 kb on + strand, within SGL_RS07705 at 1246.389 kb on - strand, within SGL_RS07705 at 1246.644 kb on - strand, within SGL_RS07705 at 1246.646 kb on + strand, within SGL_RS07705 at 1246.726 kb on + strand, within SGL_RS07705 at 1246.734 kb on - strand, within SGL_RS07705
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,244,414 - -3.0 1,244,552 + SGL_RS19585 0.68 -5.9 1,244,621 + -2.8 1,244,628 - -2.2 1,244,835 - SGL_RS07695 0.18 +0.3 1,244,882 - SGL_RS07695 0.26 +0.2 1,244,989 - SGL_RS07695 0.45 +0.7 1,245,080 - SGL_RS07695 0.60 -0.1 1,245,094 + SGL_RS07695 0.63 -0.3 1,245,125 + SGL_RS07695 0.68 -0.8 1,245,141 + SGL_RS07695 0.71 +0.0 1,245,148 + SGL_RS07695 0.72 -0.0 1,245,207 + SGL_RS07695 0.82 -0.6 1,245,339 + +0.6 1,245,730 - SGL_RS07700 0.72 +1.3 1,245,854 + -0.0 1,245,861 - +0.5 1,246,125 + SGL_RS07705 0.20 -0.8 1,246,326 - SGL_RS07705 0.37 -1.1 1,246,336 + SGL_RS07705 0.38 -0.3 1,246,381 + SGL_RS07705 0.42 -1.0 1,246,389 - SGL_RS07705 0.43 -0.5 1,246,644 - SGL_RS07705 0.65 -1.1 1,246,646 + SGL_RS07705 0.65 -1.7 1,246,726 + SGL_RS07705 0.72 -0.2 1,246,734 - SGL_RS07705 0.73 -0.7
Or see this region's nucleotide sequence