Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07200 and SGL_RS07205 are separated by 116 nucleotides SGL_RS07205 and SGL_RS07210 are separated by 148 nucleotides SGL_RS07210 and SGL_RS07215 are separated by 32 nucleotides
SGL_RS07200: SGL_RS07200 - LCP family protein, at 1,144,728 to 1,146,173
_RS07200
SGL_RS07205: SGL_RS07205 - hypothetical protein, at 1,146,290 to 1,146,718
_RS07205
SGL_RS07210: SGL_RS07210 - hypothetical protein, at 1,146,867 to 1,147,046
_RS07210
SGL_RS07215: SGL_RS07215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 1,147,079 to 1,148,098
_RS07215
Position (kb)
1146
1147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1145.301 kb on + strand, within SGL_RS07200 at 1145.305 kb on - strand, within SGL_RS07200 at 1145.757 kb on - strand, within SGL_RS07200 at 1145.815 kb on - strand, within SGL_RS07200 at 1146.123 kb on - strand at 1146.154 kb on + strand at 1146.167 kb on + strand at 1146.368 kb on - strand, within SGL_RS07205 at 1146.485 kb on - strand, within SGL_RS07205 at 1146.540 kb on + strand, within SGL_RS07205 at 1146.601 kb on - strand, within SGL_RS07205 at 1146.601 kb on - strand, within SGL_RS07205 at 1146.683 kb on - strand at 1146.752 kb on - strand at 1146.776 kb on - strand at 1146.840 kb on - strand at 1146.900 kb on - strand, within SGL_RS07210 at 1146.977 kb on + strand, within SGL_RS07210 at 1147.001 kb on + strand, within SGL_RS07210 at 1147.003 kb on + strand, within SGL_RS07210 at 1147.158 kb on + strand at 1147.181 kb on + strand, within SGL_RS07215 at 1147.188 kb on + strand, within SGL_RS07215 at 1147.265 kb on + strand, within SGL_RS07215 at 1147.327 kb on - strand, within SGL_RS07215 at 1147.487 kb on - strand, within SGL_RS07215 at 1147.576 kb on - strand, within SGL_RS07215 at 1147.684 kb on - strand, within SGL_RS07215 at 1147.688 kb on + strand, within SGL_RS07215 at 1147.696 kb on - strand, within SGL_RS07215
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 1,145,301 + SGL_RS07200 0.40 -1.2 1,145,305 - SGL_RS07200 0.40 -2.5 1,145,757 - SGL_RS07200 0.71 -2.0 1,145,815 - SGL_RS07200 0.75 -0.2 1,146,123 - -1.8 1,146,154 + -0.3 1,146,167 + -0.0 1,146,368 - SGL_RS07205 0.18 +2.5 1,146,485 - SGL_RS07205 0.45 +2.2 1,146,540 + SGL_RS07205 0.58 +2.2 1,146,601 - SGL_RS07205 0.72 +2.0 1,146,601 - SGL_RS07205 0.72 +2.6 1,146,683 - +0.9 1,146,752 - +0.8 1,146,776 - -0.1 1,146,840 - +0.0 1,146,900 - SGL_RS07210 0.18 -0.7 1,146,977 + SGL_RS07210 0.61 -0.1 1,147,001 + SGL_RS07210 0.74 +0.0 1,147,003 + SGL_RS07210 0.76 +0.4 1,147,158 + +0.1 1,147,181 + SGL_RS07215 0.10 -0.4 1,147,188 + SGL_RS07215 0.11 -0.1 1,147,265 + SGL_RS07215 0.18 -0.4 1,147,327 - SGL_RS07215 0.24 +0.2 1,147,487 - SGL_RS07215 0.40 +0.1 1,147,576 - SGL_RS07215 0.49 +0.2 1,147,684 - SGL_RS07215 0.59 -0.0 1,147,688 + SGL_RS07215 0.60 +0.0 1,147,696 - SGL_RS07215 0.60 +0.2
Or see this region's nucleotide sequence