Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06265 and SGL_RS06270 are separated by 132 nucleotides SGL_RS06270 and SGL_RS06275 are separated by 30 nucleotides SGL_RS06275 and SGL_RS06280 are separated by 127 nucleotides
SGL_RS06265: SGL_RS06265 - class III poly(R)-hydroxyalkanoic acid synthase subunit PhaE, at 932,071 to 933,063
_RS06265
SGL_RS06270: SGL_RS06270 - class III poly(R)-hydroxyalkanoic acid synthase subunit PhaC, at 933,196 to 934,293
_RS06270
SGL_RS06275: SGL_RS06275 - hypothetical protein, at 934,324 to 934,707
_RS06275
SGL_RS06280: SGL_RS06280 - fasciclin domain-containing protein, at 934,835 to 935,236
_RS06280
Position (kb) 933
934
935 Strain fitness (log2 ratio) -1
0
1
2
3 at 932.217 kb on + strand, within SGL_RS06265 at 932.283 kb on + strand, within SGL_RS06265 at 932.341 kb on + strand, within SGL_RS06265 at 932.349 kb on - strand, within SGL_RS06265 at 932.367 kb on + strand, within SGL_RS06265 at 932.431 kb on - strand, within SGL_RS06265 at 932.477 kb on + strand, within SGL_RS06265 at 932.495 kb on - strand, within SGL_RS06265 at 932.495 kb on - strand, within SGL_RS06265 at 932.621 kb on + strand, within SGL_RS06265 at 932.671 kb on - strand, within SGL_RS06265 at 932.679 kb on + strand, within SGL_RS06265 at 932.768 kb on - strand, within SGL_RS06265 at 933.326 kb on + strand, within SGL_RS06270 at 933.418 kb on + strand, within SGL_RS06270 at 934.190 kb on - strand at 934.313 kb on - strand at 934.330 kb on + strand at 934.424 kb on - strand, within SGL_RS06275 at 934.646 kb on + strand, within SGL_RS06275 at 934.657 kb on + strand, within SGL_RS06275 at 934.789 kb on - strand at 934.810 kb on - strand at 935.031 kb on + strand, within SGL_RS06280 at 935.110 kb on - strand, within SGL_RS06280 at 935.142 kb on + strand, within SGL_RS06280 at 935.142 kb on - strand, within SGL_RS06280 at 935.142 kb on - strand, within SGL_RS06280 at 935.142 kb on - strand, within SGL_RS06280
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 932,217 + SGL_RS06265 0.15 +0.2 932,283 + SGL_RS06265 0.21 +0.4 932,341 + SGL_RS06265 0.27 +0.3 932,349 - SGL_RS06265 0.28 +0.4 932,367 + SGL_RS06265 0.30 -1.0 932,431 - SGL_RS06265 0.36 -0.0 932,477 + SGL_RS06265 0.41 -1.3 932,495 - SGL_RS06265 0.43 -1.2 932,495 - SGL_RS06265 0.43 +0.2 932,621 + SGL_RS06265 0.55 +0.3 932,671 - SGL_RS06265 0.60 -0.3 932,679 + SGL_RS06265 0.61 -0.5 932,768 - SGL_RS06265 0.70 -1.0 933,326 + SGL_RS06270 0.12 +1.0 933,418 + SGL_RS06270 0.20 +1.0 934,190 - -0.9 934,313 - +0.2 934,330 + +0.5 934,424 - SGL_RS06275 0.26 +0.4 934,646 + SGL_RS06275 0.84 +3.4 934,657 + SGL_RS06275 0.87 +1.3 934,789 - -0.9 934,810 - -0.8 935,031 + SGL_RS06280 0.49 +0.1 935,110 - SGL_RS06280 0.68 +0.1 935,142 + SGL_RS06280 0.76 -0.3 935,142 - SGL_RS06280 0.76 +0.6 935,142 - SGL_RS06280 0.76 -0.7 935,142 - SGL_RS06280 0.76 +0.4
Or see this region's nucleotide sequence