Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06250 and SGL_RS06255 are separated by 63 nucleotides SGL_RS06255 and SGL_RS20120 are separated by 64 nucleotides SGL_RS20120 and SGL_RS06260 are separated by 62 nucleotides SGL_RS06260 and SGL_RS06265 are separated by 111 nucleotides
SGL_RS06250: SGL_RS06250 - hypothetical protein, at 930,148 to 930,843
_RS06250
SGL_RS06255: SGL_RS06255 - Tic20 family protein, at 930,907 to 931,389
_RS06255
SGL_RS20120: SGL_RS20120 - hypothetical protein, at 931,454 to 931,576
_RS20120
SGL_RS06260: SGL_RS06260 - 2Fe-2S iron-sulfur cluster-binding protein, at 931,639 to 931,959
_RS06260
SGL_RS06265: SGL_RS06265 - class III poly(R)-hydroxyalkanoic acid synthase subunit PhaE, at 932,071 to 933,063
_RS06265
Position (kb) 930
931
932 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 930.047 kb on - strand at 930.179 kb on - strand at 930.181 kb on - strand at 930.217 kb on + strand at 930.223 kb on + strand, within SGL_RS06250 at 930.225 kb on - strand, within SGL_RS06250 at 930.231 kb on - strand, within SGL_RS06250 at 930.453 kb on - strand, within SGL_RS06250 at 930.504 kb on - strand, within SGL_RS06250 at 930.563 kb on - strand, within SGL_RS06250 at 930.763 kb on + strand, within SGL_RS06250 at 930.964 kb on + strand, within SGL_RS06255 at 930.972 kb on - strand, within SGL_RS06255 at 931.051 kb on - strand, within SGL_RS06255 at 931.054 kb on + strand, within SGL_RS06255 at 931.141 kb on - strand, within SGL_RS06255 at 931.540 kb on + strand, within SGL_RS20120 at 931.558 kb on - strand, within SGL_RS20120 at 931.641 kb on + strand at 931.655 kb on - strand at 931.675 kb on + strand, within SGL_RS06260 at 931.682 kb on + strand, within SGL_RS06260 at 931.688 kb on - strand, within SGL_RS06260 at 931.712 kb on - strand, within SGL_RS06260 at 931.771 kb on - strand, within SGL_RS06260 at 931.778 kb on - strand, within SGL_RS06260 at 931.843 kb on + strand, within SGL_RS06260 at 931.864 kb on + strand, within SGL_RS06260 at 931.872 kb on - strand, within SGL_RS06260 at 931.877 kb on - strand, within SGL_RS06260 at 931.973 kb on - strand at 932.129 kb on - strand at 932.166 kb on + strand at 932.180 kb on - strand, within SGL_RS06265 at 932.217 kb on + strand, within SGL_RS06265 at 932.283 kb on + strand, within SGL_RS06265 at 932.341 kb on + strand, within SGL_RS06265 at 932.349 kb on - strand, within SGL_RS06265 at 932.367 kb on + strand, within SGL_RS06265
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 930,047 - +0.3 930,179 - +0.6 930,181 - -0.1 930,217 + -0.1 930,223 + SGL_RS06250 0.11 +0.4 930,225 - SGL_RS06250 0.11 +0.3 930,231 - SGL_RS06250 0.12 +0.3 930,453 - SGL_RS06250 0.44 -0.2 930,504 - SGL_RS06250 0.51 -1.1 930,563 - SGL_RS06250 0.60 -0.6 930,763 + SGL_RS06250 0.88 -0.5 930,964 + SGL_RS06255 0.12 +1.8 930,972 - SGL_RS06255 0.13 +1.4 931,051 - SGL_RS06255 0.30 +1.0 931,054 + SGL_RS06255 0.30 +1.4 931,141 - SGL_RS06255 0.48 +1.3 931,540 + SGL_RS20120 0.70 -0.1 931,558 - SGL_RS20120 0.85 -0.4 931,641 + -1.1 931,655 - +0.2 931,675 + SGL_RS06260 0.11 -0.8 931,682 + SGL_RS06260 0.13 -0.1 931,688 - SGL_RS06260 0.15 -0.1 931,712 - SGL_RS06260 0.23 -0.3 931,771 - SGL_RS06260 0.41 -1.2 931,778 - SGL_RS06260 0.43 +0.6 931,843 + SGL_RS06260 0.64 -0.9 931,864 + SGL_RS06260 0.70 -0.5 931,872 - SGL_RS06260 0.73 -3.3 931,877 - SGL_RS06260 0.74 +1.1 931,973 - +0.0 932,129 - +0.5 932,166 + +0.2 932,180 - SGL_RS06265 0.11 -0.3 932,217 + SGL_RS06265 0.15 +0.2 932,283 + SGL_RS06265 0.21 +0.4 932,341 + SGL_RS06265 0.27 +0.3 932,349 - SGL_RS06265 0.28 +0.4 932,367 + SGL_RS06265 0.30 -1.0
Or see this region's nucleotide sequence