Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06135 and SGL_RS06140 are separated by 362 nucleotides SGL_RS06140 and SGL_RS06145 are separated by 412 nucleotides
SGL_RS06135: SGL_RS06135 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 909,603 to 910,994
_RS06135
SGL_RS06140: SGL_RS06140 - threonine ammonia-lyase, biosynthetic, at 911,357 to 912,883
_RS06140
SGL_RS06145: SGL_RS06145 - cell division protein SepF, at 913,296 to 913,856
_RS06145
Position (kb)
911
912
913 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 910.364 kb on + strand, within SGL_RS06135 at 910.364 kb on + strand, within SGL_RS06135 at 910.372 kb on - strand, within SGL_RS06135 at 910.401 kb on + strand, within SGL_RS06135 at 910.521 kb on - strand, within SGL_RS06135 at 910.565 kb on - strand, within SGL_RS06135 at 910.595 kb on - strand, within SGL_RS06135 at 910.605 kb on + strand, within SGL_RS06135 at 910.607 kb on + strand, within SGL_RS06135 at 910.615 kb on - strand, within SGL_RS06135 at 910.623 kb on - strand, within SGL_RS06135 at 910.658 kb on - strand, within SGL_RS06135 at 910.747 kb on - strand, within SGL_RS06135 at 910.758 kb on + strand, within SGL_RS06135 at 910.774 kb on - strand, within SGL_RS06135 at 910.856 kb on - strand at 911.037 kb on + strand at 911.203 kb on - strand at 911.272 kb on + strand at 911.272 kb on + strand at 911.277 kb on + strand at 911.280 kb on - strand at 911.285 kb on - strand at 911.403 kb on - strand at 911.597 kb on - strand, within SGL_RS06140 at 911.597 kb on - strand, within SGL_RS06140 at 911.605 kb on + strand, within SGL_RS06140 at 911.666 kb on - strand, within SGL_RS06140 at 911.735 kb on + strand, within SGL_RS06140 at 911.735 kb on + strand, within SGL_RS06140 at 911.863 kb on - strand, within SGL_RS06140 at 911.905 kb on - strand, within SGL_RS06140 at 911.922 kb on + strand, within SGL_RS06140 at 911.930 kb on - strand, within SGL_RS06140 at 912.048 kb on + strand, within SGL_RS06140 at 912.050 kb on + strand, within SGL_RS06140 at 912.156 kb on + strand, within SGL_RS06140 at 912.176 kb on + strand, within SGL_RS06140 at 912.330 kb on - strand, within SGL_RS06140 at 912.334 kb on + strand, within SGL_RS06140 at 912.406 kb on - strand, within SGL_RS06140 at 912.479 kb on + strand, within SGL_RS06140 at 912.524 kb on - strand, within SGL_RS06140 at 912.598 kb on - strand, within SGL_RS06140 at 912.696 kb on + strand, within SGL_RS06140 at 912.709 kb on - strand, within SGL_RS06140 at 912.812 kb on - strand at 912.821 kb on + strand at 912.948 kb on + strand at 912.948 kb on + strand at 912.977 kb on - strand at 913.803 kb on - strand at 913.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 910,364 + SGL_RS06135 0.55 +0.4 910,364 + SGL_RS06135 0.55 -0.3 910,372 - SGL_RS06135 0.55 +0.2 910,401 + SGL_RS06135 0.57 +0.6 910,521 - SGL_RS06135 0.66 +2.4 910,565 - SGL_RS06135 0.69 -0.1 910,595 - SGL_RS06135 0.71 +0.7 910,605 + SGL_RS06135 0.72 +0.6 910,607 + SGL_RS06135 0.72 +0.3 910,615 - SGL_RS06135 0.73 +0.2 910,623 - SGL_RS06135 0.73 +0.4 910,658 - SGL_RS06135 0.76 -0.1 910,747 - SGL_RS06135 0.82 +0.6 910,758 + SGL_RS06135 0.83 +0.5 910,774 - SGL_RS06135 0.84 +0.8 910,856 - +0.9 911,037 + +0.6 911,203 - +1.4 911,272 + -1.2 911,272 + -0.5 911,277 + +0.3 911,280 - -0.1 911,285 - +1.2 911,403 - -0.1 911,597 - SGL_RS06140 0.16 -0.5 911,597 - SGL_RS06140 0.16 +1.0 911,605 + SGL_RS06140 0.16 +2.0 911,666 - SGL_RS06140 0.20 +0.9 911,735 + SGL_RS06140 0.25 +0.2 911,735 + SGL_RS06140 0.25 +0.9 911,863 - SGL_RS06140 0.33 +0.4 911,905 - SGL_RS06140 0.36 +1.1 911,922 + SGL_RS06140 0.37 +0.6 911,930 - SGL_RS06140 0.38 +1.1 912,048 + SGL_RS06140 0.45 +1.8 912,050 + SGL_RS06140 0.45 +1.1 912,156 + SGL_RS06140 0.52 +0.6 912,176 + SGL_RS06140 0.54 +1.3 912,330 - SGL_RS06140 0.64 +1.5 912,334 + SGL_RS06140 0.64 -0.4 912,406 - SGL_RS06140 0.69 -1.1 912,479 + SGL_RS06140 0.73 +0.9 912,524 - SGL_RS06140 0.76 +0.6 912,598 - SGL_RS06140 0.81 +1.0 912,696 + SGL_RS06140 0.88 +0.4 912,709 - SGL_RS06140 0.89 +1.3 912,812 - +0.2 912,821 + +0.4 912,948 + -1.4 912,948 + -1.7 912,977 - -0.7 913,803 - -2.5 913,869 + -1.1
Or see this region's nucleotide sequence