Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05445 and SGL_RS05450 are separated by 66 nucleotides SGL_RS05450 and SGL_RS05455 are separated by 92 nucleotides SGL_RS05455 and SGL_RS05460 overlap by 4 nucleotides SGL_RS05460 and SGL_RS05465 are separated by 39 nucleotides
SGL_RS05445: SGL_RS05445 - response regulator, at 767,890 to 768,432
_RS05445
SGL_RS05450: SGL_RS05450 - YbjN domain-containing protein, at 768,499 to 768,960
_RS05450
SGL_RS05455: SGL_RS05455 - radical SAM family heme chaperone HemW, at 769,053 to 770,291
_RS05455
SGL_RS05460: SGL_RS05460 - nicotinate-nucleotide adenylyltransferase, at 770,288 to 770,890
_RS05460
SGL_RS05465: SGL_RS05465 - hypothetical protein, at 770,930 to 771,481
_RS05465
Position (kb) 769
770
771 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 768.255 kb on - strand, within SGL_RS05445 at 768.525 kb on - strand at 768.565 kb on - strand, within SGL_RS05450 at 768.639 kb on + strand, within SGL_RS05450 at 768.647 kb on - strand, within SGL_RS05450 at 768.709 kb on + strand, within SGL_RS05450 at 769.042 kb on - strand at 769.183 kb on - strand, within SGL_RS05455 at 769.312 kb on - strand, within SGL_RS05455 at 769.325 kb on - strand, within SGL_RS05455 at 769.354 kb on - strand, within SGL_RS05455 at 769.448 kb on + strand, within SGL_RS05455 at 769.511 kb on - strand, within SGL_RS05455 at 769.542 kb on + strand, within SGL_RS05455 at 769.575 kb on + strand, within SGL_RS05455 at 769.583 kb on + strand, within SGL_RS05455 at 769.677 kb on + strand, within SGL_RS05455 at 769.688 kb on + strand, within SGL_RS05455 at 769.691 kb on - strand, within SGL_RS05455 at 769.888 kb on + strand, within SGL_RS05455 at 770.113 kb on - strand, within SGL_RS05455 at 770.391 kb on + strand, within SGL_RS05460 at 771.248 kb on - strand, within SGL_RS05465 at 771.265 kb on - strand, within SGL_RS05465
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 768,255 - SGL_RS05445 0.67 +3.2 768,525 - -0.8 768,565 - SGL_RS05450 0.14 -0.8 768,639 + SGL_RS05450 0.30 +0.3 768,647 - SGL_RS05450 0.32 +0.7 768,709 + SGL_RS05450 0.45 -0.7 769,042 - -2.4 769,183 - SGL_RS05455 0.10 +0.0 769,312 - SGL_RS05455 0.21 +1.4 769,325 - SGL_RS05455 0.22 +0.1 769,354 - SGL_RS05455 0.24 +1.4 769,448 + SGL_RS05455 0.32 +1.0 769,511 - SGL_RS05455 0.37 +0.5 769,542 + SGL_RS05455 0.39 +0.7 769,575 + SGL_RS05455 0.42 +0.8 769,583 + SGL_RS05455 0.43 +1.3 769,677 + SGL_RS05455 0.50 +1.1 769,688 + SGL_RS05455 0.51 +0.8 769,691 - SGL_RS05455 0.51 +1.3 769,888 + SGL_RS05455 0.67 +1.0 770,113 - SGL_RS05455 0.86 +1.4 770,391 + SGL_RS05460 0.17 -0.2 771,248 - SGL_RS05465 0.58 -2.0 771,265 - SGL_RS05465 0.61 -1.2
Or see this region's nucleotide sequence