Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04355 and SGL_RS04360 are separated by 267 nucleotides SGL_RS04360 and SGL_RS04365 are separated by 156 nucleotides SGL_RS04365 and SGL_RS04370 are separated by 99 nucleotides
SGL_RS04355: SGL_RS04355 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 538,415 to 539,020
_RS04355
SGL_RS04360: SGL_RS04360 - 5-formyltetrahydrofolate cyclo-ligase, at 539,288 to 539,854
_RS04360
SGL_RS04365: SGL_RS04365 - HAD family hydrolase, at 540,011 to 540,763
_RS04365
SGL_RS04370: SGL_RS04370 - class I SAM-dependent methyltransferase, at 540,863 to 542,065
_RS04370
Position (kb) 540
541 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 539.061 kb on - strand at 539.120 kb on - strand at 539.215 kb on + strand at 539.348 kb on - strand, within SGL_RS04360 at 539.545 kb on + strand, within SGL_RS04360 at 539.557 kb on + strand, within SGL_RS04360 at 539.570 kb on + strand, within SGL_RS04360 at 539.587 kb on - strand, within SGL_RS04360 at 539.601 kb on + strand, within SGL_RS04360 at 539.706 kb on - strand, within SGL_RS04360 at 539.884 kb on + strand at 539.890 kb on - strand at 539.892 kb on - strand at 539.894 kb on + strand at 539.894 kb on + strand at 539.932 kb on + strand at 539.981 kb on - strand at 539.983 kb on - strand at 539.983 kb on - strand at 539.993 kb on - strand at 540.026 kb on + strand at 540.133 kb on - strand, within SGL_RS04365 at 540.153 kb on - strand, within SGL_RS04365 at 540.163 kb on - strand, within SGL_RS04365 at 540.187 kb on - strand, within SGL_RS04365 at 540.210 kb on - strand, within SGL_RS04365 at 540.236 kb on - strand, within SGL_RS04365 at 540.246 kb on + strand, within SGL_RS04365 at 540.301 kb on - strand, within SGL_RS04365 at 540.333 kb on + strand, within SGL_RS04365 at 540.368 kb on + strand, within SGL_RS04365 at 540.376 kb on - strand, within SGL_RS04365 at 540.425 kb on - strand, within SGL_RS04365 at 540.453 kb on - strand, within SGL_RS04365 at 540.466 kb on - strand, within SGL_RS04365 at 540.486 kb on - strand, within SGL_RS04365 at 540.486 kb on - strand, within SGL_RS04365 at 540.486 kb on - strand, within SGL_RS04365 at 540.497 kb on + strand, within SGL_RS04365 at 540.505 kb on - strand, within SGL_RS04365 at 540.574 kb on - strand, within SGL_RS04365 at 540.651 kb on - strand, within SGL_RS04365 at 540.651 kb on - strand, within SGL_RS04365 at 540.679 kb on + strand, within SGL_RS04365 at 540.690 kb on + strand at 540.697 kb on - strand at 540.757 kb on + strand at 540.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 539,061 - +0.4 539,120 - -0.0 539,215 + -0.0 539,348 - SGL_RS04360 0.11 -2.9 539,545 + SGL_RS04360 0.45 -2.2 539,557 + SGL_RS04360 0.47 -2.3 539,570 + SGL_RS04360 0.50 -5.6 539,587 - SGL_RS04360 0.53 -3.1 539,601 + SGL_RS04360 0.55 -4.2 539,706 - SGL_RS04360 0.74 -4.2 539,884 + -1.0 539,890 - -0.0 539,892 - -1.0 539,894 + +0.4 539,894 + +0.9 539,932 + -1.0 539,981 - +0.2 539,983 - -0.8 539,983 - +0.1 539,993 - +0.6 540,026 + -5.3 540,133 - SGL_RS04365 0.16 -2.2 540,153 - SGL_RS04365 0.19 -5.1 540,163 - SGL_RS04365 0.20 -4.2 540,187 - SGL_RS04365 0.23 -3.2 540,210 - SGL_RS04365 0.26 -0.7 540,236 - SGL_RS04365 0.30 -4.1 540,246 + SGL_RS04365 0.31 -3.1 540,301 - SGL_RS04365 0.39 -2.7 540,333 + SGL_RS04365 0.43 -4.7 540,368 + SGL_RS04365 0.47 -4.1 540,376 - SGL_RS04365 0.48 -4.0 540,425 - SGL_RS04365 0.55 -3.9 540,453 - SGL_RS04365 0.59 -5.1 540,466 - SGL_RS04365 0.60 -4.2 540,486 - SGL_RS04365 0.63 -4.8 540,486 - SGL_RS04365 0.63 -4.9 540,486 - SGL_RS04365 0.63 -1.9 540,497 + SGL_RS04365 0.65 -4.0 540,505 - SGL_RS04365 0.66 -4.3 540,574 - SGL_RS04365 0.75 -4.5 540,651 - SGL_RS04365 0.85 -3.4 540,651 - SGL_RS04365 0.85 -4.6 540,679 + SGL_RS04365 0.89 -5.5 540,690 + -2.7 540,697 - -3.7 540,757 + +0.5 540,765 - +0.2
Or see this region's nucleotide sequence