Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04200 and SGL_RS04205 are separated by 46 nucleotides SGL_RS04205 and SGL_RS04210 are separated by 70 nucleotides SGL_RS04210 and SGL_RS04215 are separated by 88 nucleotides
SGL_RS04200: SGL_RS04200 - response regulator, at 491,084 to 491,449
_RS04200
SGL_RS04205: SGL_RS04205 - chemotaxis protein CheW, at 491,496 to 492,059
_RS04205
SGL_RS04210: SGL_RS04210 - methyl-accepting chemotaxis protein, at 492,130 to 494,739
_RS04210
SGL_RS04215: SGL_RS04215 - MlaE family lipid ABC transporter permease subunit, at 494,828 to 495,619
_RS04215
Position (kb)
492
493
494
495 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 491.130 kb on - strand, within SGL_RS04200 at 491.222 kb on + strand, within SGL_RS04200 at 491.574 kb on + strand, within SGL_RS04205 at 491.686 kb on + strand, within SGL_RS04205 at 491.712 kb on + strand, within SGL_RS04205 at 491.712 kb on + strand, within SGL_RS04205 at 491.736 kb on - strand, within SGL_RS04205 at 491.965 kb on - strand, within SGL_RS04205 at 492.032 kb on + strand at 492.044 kb on - strand at 492.101 kb on + strand at 492.109 kb on - strand at 492.168 kb on + strand at 492.168 kb on + strand at 492.189 kb on + strand at 492.523 kb on + strand, within SGL_RS04210 at 492.531 kb on - strand, within SGL_RS04210 at 492.570 kb on - strand, within SGL_RS04210 at 492.606 kb on + strand, within SGL_RS04210 at 492.614 kb on - strand, within SGL_RS04210 at 492.679 kb on + strand, within SGL_RS04210 at 492.880 kb on + strand, within SGL_RS04210 at 492.886 kb on + strand, within SGL_RS04210 at 492.886 kb on + strand, within SGL_RS04210 at 492.891 kb on + strand, within SGL_RS04210 at 492.925 kb on + strand, within SGL_RS04210 at 492.933 kb on - strand, within SGL_RS04210 at 492.933 kb on - strand, within SGL_RS04210 at 492.933 kb on - strand, within SGL_RS04210 at 493.053 kb on - strand, within SGL_RS04210 at 493.053 kb on - strand, within SGL_RS04210 at 493.118 kb on - strand, within SGL_RS04210 at 493.206 kb on + strand, within SGL_RS04210 at 493.258 kb on - strand, within SGL_RS04210 at 493.290 kb on - strand, within SGL_RS04210 at 493.297 kb on + strand, within SGL_RS04210 at 493.315 kb on + strand, within SGL_RS04210 at 493.422 kb on + strand, within SGL_RS04210 at 493.423 kb on + strand, within SGL_RS04210 at 493.461 kb on - strand, within SGL_RS04210 at 493.471 kb on + strand, within SGL_RS04210 at 493.495 kb on + strand, within SGL_RS04210 at 493.509 kb on - strand, within SGL_RS04210 at 493.519 kb on + strand, within SGL_RS04210 at 493.836 kb on + strand, within SGL_RS04210 at 493.836 kb on + strand, within SGL_RS04210 at 493.844 kb on - strand, within SGL_RS04210 at 493.941 kb on + strand, within SGL_RS04210 at 494.020 kb on - strand, within SGL_RS04210 at 494.079 kb on + strand, within SGL_RS04210 at 494.164 kb on + strand, within SGL_RS04210 at 494.263 kb on - strand, within SGL_RS04210 at 494.482 kb on + strand at 494.485 kb on - strand at 494.485 kb on - strand at 494.515 kb on - strand at 494.595 kb on + strand at 494.646 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.745 kb on + strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.946 kb on + strand, within SGL_RS04215 at 494.956 kb on - strand, within SGL_RS04215 at 495.038 kb on - strand, within SGL_RS04215 at 495.094 kb on - strand, within SGL_RS04215 at 495.272 kb on + strand, within SGL_RS04215 at 495.280 kb on - strand, within SGL_RS04215 at 495.400 kb on + strand, within SGL_RS04215 at 495.504 kb on - strand, within SGL_RS04215 at 495.532 kb on + strand, within SGL_RS04215 at 495.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 491,130 - SGL_RS04200 0.13 -1.6 491,222 + SGL_RS04200 0.38 -1.6 491,574 + SGL_RS04205 0.14 +2.0 491,686 + SGL_RS04205 0.34 +2.5 491,712 + SGL_RS04205 0.38 +3.2 491,712 + SGL_RS04205 0.38 +2.7 491,736 - SGL_RS04205 0.43 +2.4 491,965 - SGL_RS04205 0.83 +2.6 492,032 + -1.9 492,044 - +1.9 492,101 + +3.0 492,109 - +0.4 492,168 + +2.5 492,168 + +3.3 492,189 + +2.0 492,523 + SGL_RS04210 0.15 +3.2 492,531 - SGL_RS04210 0.15 +3.0 492,570 - SGL_RS04210 0.17 +2.1 492,606 + SGL_RS04210 0.18 +3.1 492,614 - SGL_RS04210 0.19 +2.6 492,679 + SGL_RS04210 0.21 +2.5 492,880 + SGL_RS04210 0.29 +2.5 492,886 + SGL_RS04210 0.29 +2.4 492,886 + SGL_RS04210 0.29 +3.3 492,891 + SGL_RS04210 0.29 +3.0 492,925 + SGL_RS04210 0.30 +3.3 492,933 - SGL_RS04210 0.31 +4.1 492,933 - SGL_RS04210 0.31 +2.5 492,933 - SGL_RS04210 0.31 +2.4 493,053 - SGL_RS04210 0.35 +2.7 493,053 - SGL_RS04210 0.35 +3.1 493,118 - SGL_RS04210 0.38 +2.1 493,206 + SGL_RS04210 0.41 +3.3 493,258 - SGL_RS04210 0.43 +2.9 493,290 - SGL_RS04210 0.44 +2.7 493,297 + SGL_RS04210 0.45 +2.2 493,315 + SGL_RS04210 0.45 +2.9 493,422 + SGL_RS04210 0.50 +2.9 493,423 + SGL_RS04210 0.50 +2.8 493,461 - SGL_RS04210 0.51 +2.7 493,471 + SGL_RS04210 0.51 +2.8 493,495 + SGL_RS04210 0.52 +2.7 493,509 - SGL_RS04210 0.53 +2.8 493,519 + SGL_RS04210 0.53 +3.1 493,836 + SGL_RS04210 0.65 +3.0 493,836 + SGL_RS04210 0.65 +2.4 493,844 - SGL_RS04210 0.66 +2.9 493,941 + SGL_RS04210 0.69 +2.1 494,020 - SGL_RS04210 0.72 +2.4 494,079 + SGL_RS04210 0.75 +3.0 494,164 + SGL_RS04210 0.78 +2.1 494,263 - SGL_RS04210 0.82 +3.3 494,482 + +2.7 494,485 - +3.0 494,485 - +3.1 494,515 - +3.0 494,595 + +1.4 494,646 + +1.7 494,740 + +0.7 494,740 + -2.1 494,740 + -1.1 494,740 + -1.8 494,740 + -1.9 494,745 + -2.1 494,748 - -0.6 494,748 - -1.8 494,748 - -2.3 494,748 - -2.2 494,946 + SGL_RS04215 0.15 -1.4 494,956 - SGL_RS04215 0.16 -1.8 495,038 - SGL_RS04215 0.27 -2.1 495,094 - SGL_RS04215 0.34 -1.0 495,272 + SGL_RS04215 0.56 -2.6 495,280 - SGL_RS04215 0.57 -2.1 495,400 + SGL_RS04215 0.72 +1.0 495,504 - SGL_RS04215 0.85 -2.0 495,532 + SGL_RS04215 0.89 -0.0 495,648 + +0.4
Or see this region's nucleotide sequence