Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19425 and SGL_RS04080 are separated by 31 nucleotides SGL_RS04080 and SGL_RS04085 are separated by 94 nucleotides SGL_RS04085 and SGL_RS04090 are separated by 163 nucleotides SGL_RS04090 and SGL_RS04095 are separated by 139 nucleotides SGL_RS04095 and SGL_RS04100 are separated by 22 nucleotides
SGL_RS19425: SGL_RS19425 - hypothetical protein, at 465,560 to 465,718
_RS19425
SGL_RS04080: SGL_RS04080 - NnrU family protein, at 465,750 to 466,493
_RS04080
SGL_RS04085: SGL_RS04085 - YlqD family protein, at 466,588 to 467,037
_RS04085
SGL_RS04090: SGL_RS04090 - photosystem I reaction center subunit XII, at 467,201 to 467,296
_RS04090
SGL_RS04095: SGL_RS04095 - hypothetical protein, at 467,436 to 467,813
_RS04095
SGL_RS04100: SGL_RS04100 - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 467,836 to 468,666
_RS04100
Position (kb) 466
467
468 Strain fitness (log2 ratio) -2
-1
0
1
2 at 465.651 kb on + strand, within SGL_RS19425 at 465.689 kb on + strand, within SGL_RS19425 at 465.788 kb on + strand at 465.845 kb on + strand, within SGL_RS04080 at 465.875 kb on - strand, within SGL_RS04080 at 465.926 kb on + strand, within SGL_RS04080 at 466.026 kb on + strand, within SGL_RS04080 at 466.037 kb on + strand, within SGL_RS04080 at 466.045 kb on - strand, within SGL_RS04080 at 466.238 kb on - strand, within SGL_RS04080 at 466.411 kb on + strand, within SGL_RS04080 at 466.442 kb on - strand at 466.459 kb on + strand at 466.549 kb on - strand at 466.612 kb on - strand at 466.640 kb on - strand, within SGL_RS04085 at 466.683 kb on - strand, within SGL_RS04085 at 466.693 kb on - strand, within SGL_RS04085 at 466.738 kb on - strand, within SGL_RS04085 at 467.031 kb on + strand at 467.040 kb on - strand at 467.049 kb on - strand at 467.153 kb on + strand at 467.221 kb on + strand, within SGL_RS04090 at 467.633 kb on - strand, within SGL_RS04095 at 467.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 465,651 + SGL_RS19425 0.57 -0.1 465,689 + SGL_RS19425 0.81 +0.7 465,788 + +0.1 465,845 + SGL_RS04080 0.13 +2.1 465,875 - SGL_RS04080 0.17 -0.0 465,926 + SGL_RS04080 0.24 +0.4 466,026 + SGL_RS04080 0.37 -0.5 466,037 + SGL_RS04080 0.39 +0.0 466,045 - SGL_RS04080 0.40 +0.7 466,238 - SGL_RS04080 0.66 +2.2 466,411 + SGL_RS04080 0.89 -0.9 466,442 - +0.3 466,459 + +0.5 466,549 - -2.5 466,612 - -1.0 466,640 - SGL_RS04085 0.12 -1.0 466,683 - SGL_RS04085 0.21 -2.6 466,693 - SGL_RS04085 0.23 -2.5 466,738 - SGL_RS04085 0.33 -2.5 467,031 + +0.1 467,040 - +0.3 467,049 - -0.2 467,153 + -0.8 467,221 + SGL_RS04090 0.21 +0.3 467,633 - SGL_RS04095 0.52 -1.6 467,816 - -0.1
Or see this region's nucleotide sequence