Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS20115 and SGL_RS03840 are separated by 168 nucleotides SGL_RS03840 and SGL_RS03845 are separated by 157 nucleotides SGL_RS03845 and SGL_RS03850 are separated by 110 nucleotides SGL_RS03850 and SGL_RS03855 are separated by 35 nucleotides
SGL_RS20115: SGL_RS20115 - hypothetical protein, at 414,577 to 414,702
_RS20115
SGL_RS03840: SGL_RS03840 - adenylyl-sulfate kinase, at 414,871 to 415,404
_RS03840
SGL_RS03845: SGL_RS03845 - MotA/TolQ/ExbB proton channel family protein, at 415,562 to 416,245
_RS03845
SGL_RS03850: SGL_RS03850 - biopolymer transporter ExbD, at 416,356 to 417,165
_RS03850
SGL_RS03855: SGL_RS03855 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 417,201 to 418,541
_RS03855
Position (kb) 415
416
417 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 414.596 kb on - strand, within SGL_RS20115 at 414.598 kb on + strand, within SGL_RS20115 at 414.612 kb on - strand, within SGL_RS20115 at 414.798 kb on + strand at 414.877 kb on - strand at 414.884 kb on + strand at 414.968 kb on - strand, within SGL_RS03840 at 415.201 kb on - strand, within SGL_RS03840 at 415.323 kb on - strand, within SGL_RS03840 at 415.344 kb on + strand, within SGL_RS03840 at 415.418 kb on - strand at 415.418 kb on - strand at 415.726 kb on + strand, within SGL_RS03845 at 415.838 kb on + strand, within SGL_RS03845 at 415.910 kb on + strand, within SGL_RS03845 at 415.913 kb on - strand, within SGL_RS03845 at 415.939 kb on - strand, within SGL_RS03845 at 416.033 kb on + strand, within SGL_RS03845 at 416.038 kb on + strand, within SGL_RS03845 at 416.103 kb on + strand, within SGL_RS03845 at 416.430 kb on - strand at 416.897 kb on + strand, within SGL_RS03850 at 416.900 kb on - strand, within SGL_RS03850 at 416.902 kb on + strand, within SGL_RS03850 at 416.935 kb on - strand, within SGL_RS03850 at 417.016 kb on + strand, within SGL_RS03850 at 417.063 kb on - strand, within SGL_RS03850 at 417.160 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 414,596 - SGL_RS20115 0.15 +0.3 414,598 + SGL_RS20115 0.17 -1.3 414,612 - SGL_RS20115 0.28 -1.0 414,798 + -4.9 414,877 - -6.5 414,884 + -0.6 414,968 - SGL_RS03840 0.18 -3.0 415,201 - SGL_RS03840 0.62 -2.2 415,323 - SGL_RS03840 0.85 -1.8 415,344 + SGL_RS03840 0.89 -0.6 415,418 - -1.8 415,418 - -2.9 415,726 + SGL_RS03845 0.24 -3.1 415,838 + SGL_RS03845 0.40 -3.6 415,910 + SGL_RS03845 0.51 -2.8 415,913 - SGL_RS03845 0.51 -3.1 415,939 - SGL_RS03845 0.55 -3.1 416,033 + SGL_RS03845 0.69 -3.9 416,038 + SGL_RS03845 0.70 -4.5 416,103 + SGL_RS03845 0.79 -4.0 416,430 - +0.0 416,897 + SGL_RS03850 0.67 -4.5 416,900 - SGL_RS03850 0.67 -3.2 416,902 + SGL_RS03850 0.67 -0.6 416,935 - SGL_RS03850 0.71 -2.9 417,016 + SGL_RS03850 0.81 -3.9 417,063 - SGL_RS03850 0.87 -1.4 417,160 - -3.2
Or see this region's nucleotide sequence