Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03310 and SGL_RS03315 are separated by 37 nucleotides SGL_RS03315 and SGL_RS03320 are separated by 236 nucleotides
SGL_RS03310: SGL_RS03310 - SUMF1/EgtB/PvdO family nonheme iron enzyme, at 306,844 to 308,076
_RS03310
SGL_RS03315: SGL_RS03315 - FAD-binding oxidoreductase, at 308,114 to 309,412
_RS03315
SGL_RS03320: SGL_RS03320 - EAL domain-containing protein, at 309,649 to 312,171
_RS03320
Position (kb)
308
309
310 Strain fitness (log2 ratio)
-1
0
1
2
3 at 307.164 kb on + strand, within SGL_RS03310 at 307.164 kb on + strand, within SGL_RS03310 at 307.164 kb on + strand, within SGL_RS03310 at 307.174 kb on - strand, within SGL_RS03310 at 307.207 kb on + strand, within SGL_RS03310 at 307.216 kb on - strand, within SGL_RS03310 at 307.245 kb on - strand, within SGL_RS03310 at 307.433 kb on + strand, within SGL_RS03310 at 307.441 kb on - strand, within SGL_RS03310 at 307.571 kb on - strand, within SGL_RS03310 at 307.608 kb on - strand, within SGL_RS03310 at 307.670 kb on - strand, within SGL_RS03310 at 307.687 kb on - strand, within SGL_RS03310 at 307.725 kb on + strand, within SGL_RS03310 at 307.887 kb on - strand, within SGL_RS03310 at 308.000 kb on + strand at 308.265 kb on - strand, within SGL_RS03315 at 308.374 kb on - strand, within SGL_RS03315 at 308.448 kb on - strand, within SGL_RS03315 at 308.450 kb on - strand, within SGL_RS03315 at 308.455 kb on - strand, within SGL_RS03315 at 308.504 kb on + strand, within SGL_RS03315 at 308.572 kb on - strand, within SGL_RS03315 at 308.624 kb on + strand, within SGL_RS03315 at 308.631 kb on - strand, within SGL_RS03315 at 308.662 kb on - strand, within SGL_RS03315 at 308.694 kb on + strand, within SGL_RS03315 at 308.771 kb on - strand, within SGL_RS03315 at 308.953 kb on + strand, within SGL_RS03315 at 308.953 kb on + strand, within SGL_RS03315 at 308.956 kb on + strand, within SGL_RS03315 at 308.979 kb on - strand, within SGL_RS03315 at 308.985 kb on + strand, within SGL_RS03315 at 309.130 kb on - strand, within SGL_RS03315 at 309.135 kb on + strand, within SGL_RS03315 at 309.143 kb on - strand, within SGL_RS03315 at 309.143 kb on - strand, within SGL_RS03315 at 309.156 kb on + strand, within SGL_RS03315 at 309.164 kb on - strand, within SGL_RS03315 at 309.274 kb on + strand, within SGL_RS03315 at 309.311 kb on - strand at 309.311 kb on - strand at 309.311 kb on - strand at 309.311 kb on - strand at 309.311 kb on - strand at 309.315 kb on - strand at 309.575 kb on + strand at 309.583 kb on - strand at 309.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 307,164 + SGL_RS03310 0.26 -0.4 307,164 + SGL_RS03310 0.26 +0.9 307,164 + SGL_RS03310 0.26 +0.3 307,174 - SGL_RS03310 0.27 +0.6 307,207 + SGL_RS03310 0.29 -0.8 307,216 - SGL_RS03310 0.30 -0.6 307,245 - SGL_RS03310 0.33 -1.0 307,433 + SGL_RS03310 0.48 -0.5 307,441 - SGL_RS03310 0.48 -0.1 307,571 - SGL_RS03310 0.59 +1.1 307,608 - SGL_RS03310 0.62 -0.4 307,670 - SGL_RS03310 0.67 +0.6 307,687 - SGL_RS03310 0.68 -0.1 307,725 + SGL_RS03310 0.71 +0.8 307,887 - SGL_RS03310 0.85 -0.5 308,000 + +1.0 308,265 - SGL_RS03315 0.12 +0.2 308,374 - SGL_RS03315 0.20 +0.8 308,448 - SGL_RS03315 0.26 +1.1 308,450 - SGL_RS03315 0.26 +2.2 308,455 - SGL_RS03315 0.26 +1.5 308,504 + SGL_RS03315 0.30 +1.5 308,572 - SGL_RS03315 0.35 +1.5 308,624 + SGL_RS03315 0.39 +1.2 308,631 - SGL_RS03315 0.40 +1.7 308,662 - SGL_RS03315 0.42 +1.7 308,694 + SGL_RS03315 0.45 +0.7 308,771 - SGL_RS03315 0.51 +2.0 308,953 + SGL_RS03315 0.65 +1.5 308,953 + SGL_RS03315 0.65 +1.8 308,956 + SGL_RS03315 0.65 +1.4 308,979 - SGL_RS03315 0.67 +1.8 308,985 + SGL_RS03315 0.67 +1.9 309,130 - SGL_RS03315 0.78 +1.3 309,135 + SGL_RS03315 0.79 +1.2 309,143 - SGL_RS03315 0.79 +1.4 309,143 - SGL_RS03315 0.79 +2.0 309,156 + SGL_RS03315 0.80 +2.6 309,164 - SGL_RS03315 0.81 +1.0 309,274 + SGL_RS03315 0.89 +0.7 309,311 - +0.6 309,311 - +1.2 309,311 - +0.8 309,311 - +1.9 309,311 - +0.8 309,315 - +1.9 309,575 + +2.0 309,583 - -0.6 309,764 + +3.0
Or see this region's nucleotide sequence