Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19750 and SGL_RS02775 are separated by 90 nucleotides SGL_RS02775 and SGL_RS02780 are separated by 98 nucleotides SGL_RS02780 and SGL_RS02785 are separated by 3 nucleotides SGL_RS02785 and SGL_RS02790 are separated by 75 nucleotides
SGL_RS19750: SGL_RS19750 - hypothetical protein, at 191,857 to 192,045
_RS19750
SGL_RS02775: SGL_RS02775 - Uma2 family endonuclease, at 192,136 to 192,783
_RS02775
SGL_RS02780: SGL_RS02780 - dihydrolipoyl dehydrogenase, at 192,882 to 194,306
_RS02780
SGL_RS02785: SGL_RS02785 - CRR6 family NdhI maturation factor, at 194,310 to 194,801
_RS02785
SGL_RS02790: SGL_RS02790 - HEAT repeat domain-containing protein, at 194,877 to 195,617
_RS02790
Position (kb) 192
193
194
195 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 191.884 kb on - strand, within SGL_RS19750 at 192.009 kb on - strand, within SGL_RS19750 at 192.048 kb on - strand at 192.360 kb on + strand, within SGL_RS02775 at 192.360 kb on + strand, within SGL_RS02775 at 192.360 kb on + strand, within SGL_RS02775 at 192.360 kb on + strand, within SGL_RS02775 at 192.360 kb on + strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.368 kb on - strand, within SGL_RS02775 at 192.637 kb on - strand, within SGL_RS02775 at 192.637 kb on - strand, within SGL_RS02775 at 192.834 kb on + strand at 192.842 kb on - strand at 194.294 kb on - strand at 194.309 kb on - strand at 194.309 kb on - strand at 194.355 kb on - strand at 194.421 kb on + strand, within SGL_RS02785 at 194.445 kb on - strand, within SGL_RS02785 at 194.667 kb on - strand, within SGL_RS02785 at 194.735 kb on - strand, within SGL_RS02785 at 194.757 kb on + strand at 194.817 kb on + strand at 194.830 kb on - strand at 194.830 kb on - strand at 195.096 kb on + strand, within SGL_RS02790 at 195.215 kb on - strand, within SGL_RS02790 at 195.220 kb on - strand, within SGL_RS02790 at 195.255 kb on + strand, within SGL_RS02790
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 191,884 - SGL_RS19750 0.14 -0.8 192,009 - SGL_RS19750 0.80 -0.0 192,048 - +0.5 192,360 + SGL_RS02775 0.35 -0.2 192,360 + SGL_RS02775 0.35 +0.6 192,360 + SGL_RS02775 0.35 -0.5 192,360 + SGL_RS02775 0.35 +0.2 192,360 + SGL_RS02775 0.35 +1.0 192,368 - SGL_RS02775 0.36 +0.1 192,368 - SGL_RS02775 0.36 -0.0 192,368 - SGL_RS02775 0.36 -0.8 192,368 - SGL_RS02775 0.36 +1.3 192,368 - SGL_RS02775 0.36 +0.2 192,368 - SGL_RS02775 0.36 +0.4 192,368 - SGL_RS02775 0.36 -0.5 192,637 - SGL_RS02775 0.77 -0.3 192,637 - SGL_RS02775 0.77 +0.0 192,834 + +0.0 192,842 - +0.2 194,294 - -4.1 194,309 - +1.1 194,309 - +1.5 194,355 - +4.8 194,421 + SGL_RS02785 0.23 +3.9 194,445 - SGL_RS02785 0.27 +3.7 194,667 - SGL_RS02785 0.73 +4.2 194,735 - SGL_RS02785 0.86 +4.0 194,757 + +3.8 194,817 + -0.2 194,830 - -0.1 194,830 - -0.0 195,096 + SGL_RS02790 0.30 +0.9 195,215 - SGL_RS02790 0.46 -0.7 195,220 - SGL_RS02790 0.46 -2.0 195,255 + SGL_RS02790 0.51 -0.7
Or see this region's nucleotide sequence