Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02545 and SGL_RS02550 are separated by 127 nucleotides SGL_RS02550 and SGL_RS02555 are separated by 0 nucleotides SGL_RS02555 and SGL_RS02560 are separated by 205 nucleotides
SGL_RS02545: SGL_RS02545 - histone deacetylase, at 146,980 to 147,894
_RS02545
SGL_RS02550: SGL_RS02550 - agmatinase, at 148,022 to 148,942
_RS02550
SGL_RS02555: SGL_RS02555 - peptidylprolyl isomerase, at 148,943 to 149,683
_RS02555
SGL_RS02560: SGL_RS02560 - photosystem I assembly protein Ycf4, at 149,889 to 150,455
_RS02560
Position (kb) 148
149 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 147.028 kb on - strand at 147.036 kb on + strand at 147.052 kb on - strand at 147.284 kb on + strand, within SGL_RS02545 at 147.301 kb on - strand, within SGL_RS02545 at 147.325 kb on + strand, within SGL_RS02545 at 147.339 kb on - strand, within SGL_RS02545 at 147.466 kb on + strand, within SGL_RS02545 at 147.728 kb on + strand, within SGL_RS02545 at 147.852 kb on + strand at 147.860 kb on - strand at 147.873 kb on + strand at 147.880 kb on + strand at 147.914 kb on + strand at 148.078 kb on - strand at 148.407 kb on + strand, within SGL_RS02550 at 148.702 kb on - strand, within SGL_RS02550 at 148.702 kb on - strand, within SGL_RS02550 at 148.702 kb on - strand, within SGL_RS02550 at 148.742 kb on + strand, within SGL_RS02550 at 148.800 kb on - strand, within SGL_RS02550 at 148.872 kb on + strand at 149.089 kb on + strand, within SGL_RS02555 at 149.125 kb on - strand, within SGL_RS02555 at 149.505 kb on + strand, within SGL_RS02555 at 149.570 kb on + strand, within SGL_RS02555 at 149.717 kb on + strand at 149.873 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 147,028 - +0.3 147,036 + -0.2 147,052 - +0.2 147,284 + SGL_RS02545 0.33 -1.2 147,301 - SGL_RS02545 0.35 +0.3 147,325 + SGL_RS02545 0.38 +0.2 147,339 - SGL_RS02545 0.39 +1.1 147,466 + SGL_RS02545 0.53 -0.6 147,728 + SGL_RS02545 0.82 +0.5 147,852 + -0.4 147,860 - -1.5 147,873 + -0.0 147,880 + -0.4 147,914 + +0.3 148,078 - +1.5 148,407 + SGL_RS02550 0.42 +2.5 148,702 - SGL_RS02550 0.74 +2.3 148,702 - SGL_RS02550 0.74 +2.0 148,702 - SGL_RS02550 0.74 +3.9 148,742 + SGL_RS02550 0.78 +2.1 148,800 - SGL_RS02550 0.84 +3.4 148,872 + +1.7 149,089 + SGL_RS02555 0.20 -0.7 149,125 - SGL_RS02555 0.25 -1.2 149,505 + SGL_RS02555 0.76 -2.7 149,570 + SGL_RS02555 0.85 -1.5 149,717 + -1.4 149,873 + +0.3
Or see this region's nucleotide sequence