Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02525 and SGL_RS02530 are separated by 82 nucleotides SGL_RS02530 and SGL_RS02540 are separated by 231 nucleotides SGL_RS02540 and SGL_RS18650 are separated by 108 nucleotides SGL_RS18650 and SGL_RS02545 are separated by 148 nucleotides
SGL_RS02525: SGL_RS02525 - hypothetical protein, at 144,554 to 145,081
_RS02525
SGL_RS02530: SGL_RS02530 - MTH1187 family thiamine-binding protein, at 145,164 to 145,529
_RS02530
SGL_RS02540: SGL_RS02540 - universal stress protein, at 145,761 to 146,615
_RS02540
SGL_RS18650: SGL_RS18650 - photosystem II reaction center protein PsbM, at 146,724 to 146,831
_RS18650
SGL_RS02545: SGL_RS02545 - histone deacetylase, at 146,980 to 147,894
_RS02545
Position (kb) 145
146
147 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 144.766 kb on + strand, within SGL_RS02525 at 144.821 kb on + strand, within SGL_RS02525 at 145.410 kb on - strand, within SGL_RS02530 at 145.499 kb on - strand at 145.564 kb on + strand at 145.585 kb on - strand at 145.596 kb on - strand at 145.659 kb on - strand at 145.665 kb on - strand at 145.671 kb on + strand at 145.672 kb on + strand at 145.805 kb on - strand at 145.814 kb on + strand at 146.011 kb on - strand, within SGL_RS02540 at 146.453 kb on + strand, within SGL_RS02540 at 146.461 kb on - strand, within SGL_RS02540 at 146.508 kb on + strand, within SGL_RS02540 at 146.553 kb on - strand at 146.691 kb on + strand at 146.812 kb on - strand, within SGL_RS18650 at 146.840 kb on - strand at 146.880 kb on - strand at 146.963 kb on + strand at 147.028 kb on - strand at 147.036 kb on + strand at 147.052 kb on - strand at 147.284 kb on + strand, within SGL_RS02545 at 147.301 kb on - strand, within SGL_RS02545 at 147.325 kb on + strand, within SGL_RS02545 at 147.339 kb on - strand, within SGL_RS02545 at 147.466 kb on + strand, within SGL_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 144,766 + SGL_RS02525 0.40 +0.4 144,821 + SGL_RS02525 0.51 -2.9 145,410 - SGL_RS02530 0.67 -1.5 145,499 - +0.6 145,564 + +0.4 145,585 - -0.7 145,596 - -0.7 145,659 - -0.7 145,665 - +1.1 145,671 + +1.1 145,672 + -0.1 145,805 - +0.7 145,814 + -0.1 146,011 - SGL_RS02540 0.29 +1.5 146,453 + SGL_RS02540 0.81 +1.1 146,461 - SGL_RS02540 0.82 +0.9 146,508 + SGL_RS02540 0.87 +0.1 146,553 - +0.2 146,691 + -0.1 146,812 - SGL_RS18650 0.81 +0.8 146,840 - +0.7 146,880 - +1.3 146,963 + +0.2 147,028 - +0.3 147,036 + -0.2 147,052 - +0.2 147,284 + SGL_RS02545 0.33 -1.2 147,301 - SGL_RS02545 0.35 +0.3 147,325 + SGL_RS02545 0.38 +0.2 147,339 - SGL_RS02545 0.39 +1.1 147,466 + SGL_RS02545 0.53 -0.6
Or see this region's nucleotide sequence