Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02195 and SGL_RS02200 are separated by 79 nucleotides SGL_RS02200 and SGL_RS02205 are separated by 86 nucleotides SGL_RS02205 and SGL_RS02210 are separated by 153 nucleotides SGL_RS02210 and SGL_RS02215 overlap by 41 nucleotides SGL_RS02215 and SGL_RS02220 are separated by 21 nucleotides
SGL_RS02195: SGL_RS02195 - nucleoside monophosphate kinase, at 75,955 to 76,500
_RS02195
SGL_RS02200: SGL_RS02200 - 4-hydroxy-tetrahydrodipicolinate reductase, at 76,580 to 77,407
_RS02200
SGL_RS02205: SGL_RS02205 - thioredoxin family protein, at 77,494 to 77,826
_RS02205
SGL_RS02210: SGL_RS02210 - DUF2442 domain-containing protein, at 77,980 to 78,246
_RS02210
SGL_RS02215: SGL_RS02215 - DUF4160 domain-containing protein, at 78,206 to 78,445
_RS02215
SGL_RS02220: SGL_RS02220 - phosphoribosylformylglycinamidine synthase subunit PurL, at 78,467 to 80,800
_RS02220
Position (kb) 77
78 Strain fitness (log2 ratio) -1
0
1
2
3 at 76.880 kb on - strand, within SGL_RS02200 at 76.880 kb on - strand, within SGL_RS02200 at 77.149 kb on + strand, within SGL_RS02200 at 77.198 kb on - strand, within SGL_RS02200 at 77.205 kb on - strand, within SGL_RS02200 at 77.440 kb on - strand at 77.505 kb on - strand at 77.523 kb on + strand at 77.624 kb on + strand, within SGL_RS02205 at 77.657 kb on + strand, within SGL_RS02205 at 77.708 kb on - strand, within SGL_RS02205 at 77.905 kb on - strand at 78.242 kb on - strand at 78.580 kb on - strand at 78.585 kb on - strand at 78.609 kb on + strand at 78.806 kb on + strand, within SGL_RS02220 at 78.806 kb on + strand, within SGL_RS02220
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 76,880 - SGL_RS02200 0.36 -1.5 76,880 - SGL_RS02200 0.36 +0.8 77,149 + SGL_RS02200 0.69 -0.7 77,198 - SGL_RS02200 0.75 -0.7 77,205 - SGL_RS02200 0.75 -1.2 77,440 - +1.1 77,505 - -1.3 77,523 + +2.9 77,624 + SGL_RS02205 0.39 +2.4 77,657 + SGL_RS02205 0.49 +2.3 77,708 - SGL_RS02205 0.64 +2.2 77,905 - -0.5 78,242 - +1.1 78,580 - -1.2 78,585 - -1.2 78,609 + -1.2 78,806 + SGL_RS02220 0.15 -1.7 78,806 + SGL_RS02220 0.15 -0.2
Or see this region's nucleotide sequence