Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01505 and SGL_RS01515 are separated by 714 nucleotides SGL_RS01515 and SGL_RS19730 are separated by 31 nucleotides SGL_RS19730 and SGL_RS19320 overlap by 53 nucleotides SGL_RS19320 and SGL_RS01525 are separated by 327 nucleotides
SGL_RS01505: SGL_RS01505 - hypothetical protein, at 26,745 to 27,242
_RS01505
SGL_RS01515: SGL_RS01515 - hypothetical protein, at 27,957 to 31,322
_RS01515
SGL_RS19730: SGL_RS19730 - DNA cytosine methyltransferase, at 31,354 to 31,461
_RS19730
SGL_RS19320: SGL_RS19320 - hypothetical protein, at 31,409 to 31,801
_RS19320
SGL_RS01525: SGL_RS01525 - cytidine deaminase, at 32,129 to 32,545
_RS01525
Position (kb)
27
28
29
30
31
32 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 28.370 kb on - strand, within SGL_RS01515 at 28.452 kb on + strand, within SGL_RS01515 at 28.452 kb on + strand, within SGL_RS01515 at 28.452 kb on + strand, within SGL_RS01515 at 28.458 kb on - strand, within SGL_RS01515 at 28.460 kb on - strand, within SGL_RS01515 at 28.460 kb on - strand, within SGL_RS01515 at 28.460 kb on - strand, within SGL_RS01515 at 28.486 kb on + strand, within SGL_RS01515 at 28.509 kb on + strand, within SGL_RS01515 at 28.585 kb on - strand, within SGL_RS01515 at 28.600 kb on + strand, within SGL_RS01515 at 28.608 kb on - strand, within SGL_RS01515 at 28.660 kb on + strand, within SGL_RS01515 at 28.693 kb on + strand, within SGL_RS01515 at 28.703 kb on + strand, within SGL_RS01515 at 28.703 kb on + strand, within SGL_RS01515 at 28.703 kb on + strand, within SGL_RS01515 at 28.726 kb on + strand, within SGL_RS01515 at 28.729 kb on - strand, within SGL_RS01515 at 28.754 kb on + strand, within SGL_RS01515 at 28.761 kb on + strand, within SGL_RS01515 at 28.762 kb on - strand, within SGL_RS01515 at 28.769 kb on - strand, within SGL_RS01515 at 28.773 kb on + strand, within SGL_RS01515 at 28.775 kb on - strand, within SGL_RS01515 at 28.860 kb on - strand, within SGL_RS01515 at 28.860 kb on - strand, within SGL_RS01515 at 28.866 kb on + strand, within SGL_RS01515 at 28.873 kb on + strand, within SGL_RS01515 at 28.874 kb on + strand, within SGL_RS01515 at 28.912 kb on + strand, within SGL_RS01515 at 28.926 kb on - strand, within SGL_RS01515 at 28.965 kb on + strand, within SGL_RS01515 at 28.965 kb on + strand, within SGL_RS01515 at 28.973 kb on - strand, within SGL_RS01515 at 28.973 kb on - strand, within SGL_RS01515 at 28.973 kb on - strand, within SGL_RS01515 at 28.984 kb on + strand, within SGL_RS01515 at 29.009 kb on - strand, within SGL_RS01515 at 29.055 kb on + strand, within SGL_RS01515 at 29.134 kb on + strand, within SGL_RS01515 at 29.183 kb on + strand, within SGL_RS01515 at 29.206 kb on - strand, within SGL_RS01515 at 29.206 kb on - strand, within SGL_RS01515 at 29.211 kb on - strand, within SGL_RS01515 at 29.285 kb on + strand, within SGL_RS01515 at 29.285 kb on + strand, within SGL_RS01515 at 29.306 kb on + strand, within SGL_RS01515 at 29.306 kb on + strand, within SGL_RS01515 at 29.312 kb on + strand, within SGL_RS01515 at 29.314 kb on - strand, within SGL_RS01515 at 29.314 kb on - strand, within SGL_RS01515 at 29.314 kb on - strand, within SGL_RS01515 at 29.314 kb on - strand, within SGL_RS01515 at 29.324 kb on - strand, within SGL_RS01515 at 29.324 kb on - strand, within SGL_RS01515 at 29.324 kb on - strand, within SGL_RS01515 at 29.349 kb on - strand, within SGL_RS01515 at 29.349 kb on - strand, within SGL_RS01515 at 29.354 kb on - strand, within SGL_RS01515 at 29.393 kb on - strand, within SGL_RS01515 at 29.491 kb on + strand, within SGL_RS01515 at 29.499 kb on - strand, within SGL_RS01515 at 29.499 kb on - strand, within SGL_RS01515 at 29.531 kb on - strand, within SGL_RS01515 at 29.598 kb on - strand, within SGL_RS01515 at 29.627 kb on - strand, within SGL_RS01515 at 29.872 kb on + strand, within SGL_RS01515 at 29.888 kb on + strand, within SGL_RS01515 at 29.891 kb on - strand, within SGL_RS01515 at 29.891 kb on - strand, within SGL_RS01515 at 29.893 kb on + strand, within SGL_RS01515 at 29.893 kb on - strand, within SGL_RS01515 at 29.901 kb on - strand, within SGL_RS01515 at 29.926 kb on + strand, within SGL_RS01515 at 30.093 kb on + strand, within SGL_RS01515 at 30.129 kb on - strand, within SGL_RS01515 at 30.154 kb on + strand, within SGL_RS01515 at 30.159 kb on + strand, within SGL_RS01515 at 30.209 kb on - strand, within SGL_RS01515 at 30.287 kb on - strand, within SGL_RS01515 at 30.299 kb on - strand, within SGL_RS01515 at 30.341 kb on + strand, within SGL_RS01515 at 30.341 kb on + strand, within SGL_RS01515 at 30.341 kb on + strand, within SGL_RS01515 at 30.349 kb on - strand, within SGL_RS01515 at 30.349 kb on - strand, within SGL_RS01515 at 30.395 kb on + strand, within SGL_RS01515 at 30.402 kb on - strand, within SGL_RS01515 at 30.431 kb on - strand, within SGL_RS01515 at 30.460 kb on - strand, within SGL_RS01515 at 30.460 kb on - strand, within SGL_RS01515 at 30.511 kb on - strand, within SGL_RS01515 at 30.616 kb on + strand, within SGL_RS01515 at 30.654 kb on + strand, within SGL_RS01515 at 30.676 kb on + strand, within SGL_RS01515 at 30.766 kb on - strand, within SGL_RS01515 at 30.804 kb on + strand, within SGL_RS01515 at 30.804 kb on + strand, within SGL_RS01515 at 30.812 kb on - strand, within SGL_RS01515 at 30.817 kb on - strand, within SGL_RS01515 at 30.833 kb on - strand, within SGL_RS01515 at 30.855 kb on + strand, within SGL_RS01515 at 30.978 kb on + strand, within SGL_RS01515 at 30.991 kb on - strand at 31.016 kb on + strand at 31.029 kb on - strand at 31.111 kb on + strand at 31.172 kb on + strand at 31.347 kb on + strand at 31.347 kb on + strand at 31.381 kb on - strand, within SGL_RS19730 at 31.387 kb on - strand, within SGL_RS19730 at 31.389 kb on - strand, within SGL_RS19730 at 31.511 kb on - strand, within SGL_RS19320 at 31.545 kb on + strand, within SGL_RS19320 at 31.587 kb on - strand, within SGL_RS19320 at 31.587 kb on - strand, within SGL_RS19320 at 31.722 kb on - strand, within SGL_RS19320 at 32.079 kb on - strand at 32.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 28,370 - SGL_RS01515 0.12 +2.6 28,452 + SGL_RS01515 0.15 +2.8 28,452 + SGL_RS01515 0.15 +2.4 28,452 + SGL_RS01515 0.15 +4.0 28,458 - SGL_RS01515 0.15 +2.4 28,460 - SGL_RS01515 0.15 +3.0 28,460 - SGL_RS01515 0.15 +3.1 28,460 - SGL_RS01515 0.15 +3.0 28,486 + SGL_RS01515 0.16 +3.1 28,509 + SGL_RS01515 0.16 +3.2 28,585 - SGL_RS01515 0.19 +3.9 28,600 + SGL_RS01515 0.19 +3.1 28,608 - SGL_RS01515 0.19 +3.4 28,660 + SGL_RS01515 0.21 +3.3 28,693 + SGL_RS01515 0.22 +2.3 28,703 + SGL_RS01515 0.22 +2.3 28,703 + SGL_RS01515 0.22 +3.2 28,703 + SGL_RS01515 0.22 +3.3 28,726 + SGL_RS01515 0.23 +3.2 28,729 - SGL_RS01515 0.23 +3.2 28,754 + SGL_RS01515 0.24 +2.6 28,761 + SGL_RS01515 0.24 +3.4 28,762 - SGL_RS01515 0.24 +3.3 28,769 - SGL_RS01515 0.24 +3.7 28,773 + SGL_RS01515 0.24 +2.4 28,775 - SGL_RS01515 0.24 +2.2 28,860 - SGL_RS01515 0.27 +3.2 28,860 - SGL_RS01515 0.27 +2.9 28,866 + SGL_RS01515 0.27 +3.3 28,873 + SGL_RS01515 0.27 +2.8 28,874 + SGL_RS01515 0.27 +1.8 28,912 + SGL_RS01515 0.28 +2.2 28,926 - SGL_RS01515 0.29 +3.2 28,965 + SGL_RS01515 0.30 +2.3 28,965 + SGL_RS01515 0.30 +3.3 28,973 - SGL_RS01515 0.30 +3.2 28,973 - SGL_RS01515 0.30 +2.8 28,973 - SGL_RS01515 0.30 +2.9 28,984 + SGL_RS01515 0.31 +2.5 29,009 - SGL_RS01515 0.31 +3.7 29,055 + SGL_RS01515 0.33 +3.3 29,134 + SGL_RS01515 0.35 +3.0 29,183 + SGL_RS01515 0.36 +3.6 29,206 - SGL_RS01515 0.37 +3.2 29,206 - SGL_RS01515 0.37 +3.2 29,211 - SGL_RS01515 0.37 +2.2 29,285 + SGL_RS01515 0.39 +3.5 29,285 + SGL_RS01515 0.39 +3.7 29,306 + SGL_RS01515 0.40 +2.6 29,306 + SGL_RS01515 0.40 +4.3 29,312 + SGL_RS01515 0.40 +3.4 29,314 - SGL_RS01515 0.40 +3.8 29,314 - SGL_RS01515 0.40 +3.2 29,314 - SGL_RS01515 0.40 +3.3 29,314 - SGL_RS01515 0.40 +3.1 29,324 - SGL_RS01515 0.41 +2.8 29,324 - SGL_RS01515 0.41 +3.5 29,324 - SGL_RS01515 0.41 +3.1 29,349 - SGL_RS01515 0.41 +3.6 29,349 - SGL_RS01515 0.41 +3.5 29,354 - SGL_RS01515 0.42 +2.5 29,393 - SGL_RS01515 0.43 +3.3 29,491 + SGL_RS01515 0.46 +3.2 29,499 - SGL_RS01515 0.46 +3.5 29,499 - SGL_RS01515 0.46 +3.7 29,531 - SGL_RS01515 0.47 +3.6 29,598 - SGL_RS01515 0.49 +2.5 29,627 - SGL_RS01515 0.50 +3.0 29,872 + SGL_RS01515 0.57 +3.5 29,888 + SGL_RS01515 0.57 +3.4 29,891 - SGL_RS01515 0.57 +3.1 29,891 - SGL_RS01515 0.57 +3.3 29,893 + SGL_RS01515 0.58 +2.9 29,893 - SGL_RS01515 0.58 +2.6 29,901 - SGL_RS01515 0.58 +3.8 29,926 + SGL_RS01515 0.58 +2.0 30,093 + SGL_RS01515 0.63 +3.1 30,129 - SGL_RS01515 0.65 +3.5 30,154 + SGL_RS01515 0.65 +3.1 30,159 + SGL_RS01515 0.65 +3.5 30,209 - SGL_RS01515 0.67 +3.3 30,287 - SGL_RS01515 0.69 +4.0 30,299 - SGL_RS01515 0.70 +3.1 30,341 + SGL_RS01515 0.71 +3.5 30,341 + SGL_RS01515 0.71 +2.8 30,341 + SGL_RS01515 0.71 +3.3 30,349 - SGL_RS01515 0.71 +2.3 30,349 - SGL_RS01515 0.71 +3.2 30,395 + SGL_RS01515 0.72 +2.2 30,402 - SGL_RS01515 0.73 +2.6 30,431 - SGL_RS01515 0.73 +3.1 30,460 - SGL_RS01515 0.74 +2.9 30,460 - SGL_RS01515 0.74 +3.4 30,511 - SGL_RS01515 0.76 +3.2 30,616 + SGL_RS01515 0.79 +0.0 30,654 + SGL_RS01515 0.80 +2.4 30,676 + SGL_RS01515 0.81 +3.0 30,766 - SGL_RS01515 0.83 +3.2 30,804 + SGL_RS01515 0.85 +0.6 30,804 + SGL_RS01515 0.85 +3.0 30,812 - SGL_RS01515 0.85 +3.1 30,817 - SGL_RS01515 0.85 +2.1 30,833 - SGL_RS01515 0.85 +3.6 30,855 + SGL_RS01515 0.86 +2.9 30,978 + SGL_RS01515 0.90 +3.4 30,991 - +3.1 31,016 + +3.0 31,029 - +3.2 31,111 + +2.7 31,172 + +3.5 31,347 + -0.5 31,347 + +0.9 31,381 - SGL_RS19730 0.25 -0.0 31,387 - SGL_RS19730 0.31 -0.1 31,389 - SGL_RS19730 0.32 +0.6 31,511 - SGL_RS19320 0.26 -0.6 31,545 + SGL_RS19320 0.35 +0.0 31,587 - SGL_RS19320 0.45 +0.3 31,587 - SGL_RS19320 0.45 +0.3 31,722 - SGL_RS19320 0.80 +0.9 32,079 - -1.0 32,089 + +0.9
Or see this region's nucleotide sequence