Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01025 and SGL_RS01030 are separated by 262 nucleotides SGL_RS01030 and SGL_RS01035 are separated by 38 nucleotides SGL_RS01035 and SGL_RS01040 are separated by 91 nucleotides SGL_RS01040 and SGL_RS01045 are separated by 23 nucleotides
SGL_RS01025: SGL_RS01025 - RAMP superfamily CRISPR-associated protein, at 80,203 to 81,933
_RS01025
SGL_RS01030: SGL_RS01030 - hypothetical protein, at 82,196 to 82,591
_RS01030
SGL_RS01035: SGL_RS01035 - type III-B CRISPR module RAMP protein Cmr4, at 82,630 to 83,409
_RS01035
SGL_RS01040: SGL_RS01040 - peptidase, at 83,501 to 83,881
_RS01040
SGL_RS01045: SGL_RS01045 - type III-B CRISPR module-associated protein Cmr3, at 83,905 to 85,017
_RS01045
Position (kb) 82
83
84 Strain fitness (log2 ratio) -1
0
1
2 at 81.654 kb on + strand, within SGL_RS01025 at 81.654 kb on + strand, within SGL_RS01025 at 81.819 kb on + strand at 81.819 kb on + strand at 82.037 kb on + strand at 82.265 kb on + strand, within SGL_RS01030 at 82.266 kb on - strand, within SGL_RS01030 at 82.289 kb on - strand, within SGL_RS01030 at 82.511 kb on + strand, within SGL_RS01030 at 82.511 kb on + strand, within SGL_RS01030 at 82.519 kb on - strand, within SGL_RS01030 at 82.519 kb on - strand, within SGL_RS01030 at 82.535 kb on + strand, within SGL_RS01030 at 82.844 kb on + strand, within SGL_RS01035 at 83.156 kb on + strand, within SGL_RS01035 at 83.218 kb on + strand, within SGL_RS01035 at 83.371 kb on + strand at 83.535 kb on + strand at 83.778 kb on + strand, within SGL_RS01040 at 83.858 kb on - strand at 83.858 kb on - strand at 83.858 kb on - strand at 83.858 kb on - strand at 83.919 kb on + strand at 84.010 kb on - strand at 84.078 kb on + strand, within SGL_RS01045 at 84.180 kb on + strand, within SGL_RS01045 at 84.185 kb on + strand, within SGL_RS01045 at 84.239 kb on + strand, within SGL_RS01045 at 84.239 kb on - strand, within SGL_RS01045 at 84.277 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.324 kb on - strand, within SGL_RS01045 at 84.324 kb on - strand, within SGL_RS01045 at 84.369 kb on - strand, within SGL_RS01045 at 84.400 kb on - strand, within SGL_RS01045
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 81,654 + SGL_RS01025 0.84 +1.3 81,654 + SGL_RS01025 0.84 +0.1 81,819 + +0.2 81,819 + +1.5 82,037 + +0.9 82,265 + SGL_RS01030 0.17 +1.9 82,266 - SGL_RS01030 0.18 +1.4 82,289 - SGL_RS01030 0.23 +1.6 82,511 + SGL_RS01030 0.80 +0.5 82,511 + SGL_RS01030 0.80 +0.8 82,519 - SGL_RS01030 0.82 +1.3 82,519 - SGL_RS01030 0.82 +0.4 82,535 + SGL_RS01030 0.86 +1.1 82,844 + SGL_RS01035 0.27 +1.9 83,156 + SGL_RS01035 0.67 +1.7 83,218 + SGL_RS01035 0.75 +1.8 83,371 + +0.6 83,535 + +0.4 83,778 + SGL_RS01040 0.73 +0.8 83,858 - +0.6 83,858 - +1.6 83,858 - +0.6 83,858 - +1.5 83,919 + +0.8 84,010 - +0.6 84,078 + SGL_RS01045 0.16 +1.1 84,180 + SGL_RS01045 0.25 +0.1 84,185 + SGL_RS01045 0.25 -0.2 84,239 + SGL_RS01045 0.30 -0.4 84,239 - SGL_RS01045 0.30 -0.5 84,277 + SGL_RS01045 0.33 +0.0 84,316 + SGL_RS01045 0.37 +0.3 84,316 + SGL_RS01045 0.37 -1.5 84,316 + SGL_RS01045 0.37 -0.4 84,316 + SGL_RS01045 0.37 +0.4 84,324 - SGL_RS01045 0.38 -0.7 84,324 - SGL_RS01045 0.38 -1.4 84,369 - SGL_RS01045 0.42 +0.8 84,400 - SGL_RS01045 0.44 +0.6
Or see this region's nucleotide sequence