Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00180 and SGL_RS00185 are separated by 21 nucleotides SGL_RS00185 and SGL_RS00190 are separated by 151 nucleotides SGL_RS00190 and SGL_RS00195 are separated by 94 nucleotides
SGL_RS00180: SGL_RS00180 - IS5 family transposase, at 38,907 to 39,641
_RS00180
SGL_RS00185: SGL_RS00185 - transposase, at 39,663 to 40,259
_RS00185
SGL_RS00190: SGL_RS00190 - sulfotransferase, at 40,411 to 41,316
_RS00190
SGL_RS00195: SGL_RS00195 - glycosyltransferase family 2 protein, at 41,411 to 42,397
_RS00195
Position (kb) 40
41
42 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 39.666 kb on + strand at 39.719 kb on - strand at 39.783 kb on + strand, within SGL_RS00185 at 39.783 kb on + strand, within SGL_RS00185 at 39.791 kb on - strand, within SGL_RS00185 at 39.791 kb on - strand, within SGL_RS00185 at 39.791 kb on - strand, within SGL_RS00185 at 39.835 kb on - strand, within SGL_RS00185 at 39.856 kb on + strand, within SGL_RS00185 at 39.859 kb on - strand, within SGL_RS00185 at 39.864 kb on - strand, within SGL_RS00185 at 39.872 kb on - strand, within SGL_RS00185 at 40.198 kb on - strand, within SGL_RS00185 at 40.365 kb on - strand at 40.424 kb on + strand at 40.482 kb on + strand at 40.483 kb on - strand at 40.509 kb on - strand, within SGL_RS00190 at 40.516 kb on + strand, within SGL_RS00190 at 40.649 kb on - strand, within SGL_RS00190 at 40.779 kb on - strand, within SGL_RS00190 at 40.789 kb on - strand, within SGL_RS00190 at 40.832 kb on + strand, within SGL_RS00190 at 41.013 kb on + strand, within SGL_RS00190 at 41.042 kb on + strand, within SGL_RS00190 at 41.046 kb on + strand, within SGL_RS00190 at 41.083 kb on - strand, within SGL_RS00190 at 41.111 kb on - strand, within SGL_RS00190 at 41.392 kb on + strand at 41.533 kb on + strand, within SGL_RS00195 at 41.608 kb on + strand, within SGL_RS00195 at 41.608 kb on + strand, within SGL_RS00195 at 41.616 kb on - strand, within SGL_RS00195 at 41.616 kb on - strand, within SGL_RS00195 at 41.616 kb on - strand, within SGL_RS00195 at 41.627 kb on - strand, within SGL_RS00195 at 41.629 kb on + strand, within SGL_RS00195 at 41.637 kb on - strand, within SGL_RS00195 at 41.656 kb on - strand, within SGL_RS00195 at 41.698 kb on - strand, within SGL_RS00195 at 41.878 kb on - strand, within SGL_RS00195 at 42.008 kb on - strand, within SGL_RS00195 at 42.180 kb on - strand, within SGL_RS00195
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 39,666 + -1.4 39,719 - +0.4 39,783 + SGL_RS00185 0.20 +0.9 39,783 + SGL_RS00185 0.20 +0.2 39,791 - SGL_RS00185 0.21 -0.1 39,791 - SGL_RS00185 0.21 -0.6 39,791 - SGL_RS00185 0.21 +0.7 39,835 - SGL_RS00185 0.29 +0.1 39,856 + SGL_RS00185 0.32 -1.2 39,859 - SGL_RS00185 0.33 +0.6 39,864 - SGL_RS00185 0.34 +2.0 39,872 - SGL_RS00185 0.35 -1.1 40,198 - SGL_RS00185 0.90 -0.6 40,365 - -2.7 40,424 + -0.5 40,482 + -2.6 40,483 - -1.4 40,509 - SGL_RS00190 0.11 -3.4 40,516 + SGL_RS00190 0.12 -0.0 40,649 - SGL_RS00190 0.26 -5.0 40,779 - SGL_RS00190 0.41 -5.1 40,789 - SGL_RS00190 0.42 -4.8 40,832 + SGL_RS00190 0.46 -4.0 41,013 + SGL_RS00190 0.66 -5.0 41,042 + SGL_RS00190 0.70 -5.8 41,046 + SGL_RS00190 0.70 -3.2 41,083 - SGL_RS00190 0.74 -5.8 41,111 - SGL_RS00190 0.77 -4.2 41,392 + +0.5 41,533 + SGL_RS00195 0.12 -4.0 41,608 + SGL_RS00195 0.20 -4.9 41,608 + SGL_RS00195 0.20 -3.7 41,616 - SGL_RS00195 0.21 -3.6 41,616 - SGL_RS00195 0.21 -4.6 41,616 - SGL_RS00195 0.21 -6.5 41,627 - SGL_RS00195 0.22 -4.7 41,629 + SGL_RS00195 0.22 -1.4 41,637 - SGL_RS00195 0.23 -5.8 41,656 - SGL_RS00195 0.25 -2.0 41,698 - SGL_RS00195 0.29 -1.4 41,878 - SGL_RS00195 0.47 -5.6 42,008 - SGL_RS00195 0.60 -3.4 42,180 - SGL_RS00195 0.78 -4.8
Or see this region's nucleotide sequence