Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11290 and SGL_RS11295 are separated by 245 nucleotides SGL_RS11295 and SGL_RS11300 are separated by 202 nucleotides SGL_RS11300 and SGL_RS11305 are separated by 8 nucleotides
SGL_RS11290: SGL_RS11290 - phosphoenolpyruvate carboxylase, at 1,994,147 to 1,997,251
_RS11290
SGL_RS11295: SGL_RS11295 - hypothetical protein, at 1,997,497 to 1,997,790
_RS11295
SGL_RS11300: SGL_RS11300 - NfeD family protein, at 1,997,993 to 1,998,451
_RS11300
SGL_RS11305: SGL_RS11305 - carboxylating nicotinate-nucleotide diphosphorylase, at 1,998,460 to 1,999,347
_RS11305
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-2
-1
0
1 at 1997.330 kb on - strand at 1997.906 kb on + strand at 1997.907 kb on - strand at 1998.029 kb on + strand at 1998.032 kb on - strand at 1998.039 kb on - strand, within SGL_RS11300 at 1998.306 kb on - strand, within SGL_RS11300 at 1998.490 kb on + strand at 1998.498 kb on - strand at 1998.508 kb on - strand at 1998.859 kb on + strand, within SGL_RS11305 at 1998.902 kb on + strand, within SGL_RS11305 at 1998.967 kb on + strand, within SGL_RS11305 at 1998.975 kb on - strand, within SGL_RS11305 at 1998.975 kb on - strand, within SGL_RS11305 at 1998.975 kb on - strand, within SGL_RS11305 at 1999.100 kb on + strand, within SGL_RS11305 at 1999.110 kb on - strand, within SGL_RS11305 at 1999.234 kb on + strand, within SGL_RS11305
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 1,997,330 - +0.2 1,997,906 + +0.2 1,997,907 - -0.0 1,998,029 + +0.2 1,998,032 - -0.4 1,998,039 - SGL_RS11300 0.10 +1.6 1,998,306 - SGL_RS11300 0.68 +0.6 1,998,490 + -0.9 1,998,498 - +0.2 1,998,508 - -1.1 1,998,859 + SGL_RS11305 0.45 -0.1 1,998,902 + SGL_RS11305 0.50 +0.2 1,998,967 + SGL_RS11305 0.57 -2.0 1,998,975 - SGL_RS11305 0.58 -1.9 1,998,975 - SGL_RS11305 0.58 -0.5 1,998,975 - SGL_RS11305 0.58 +0.1 1,999,100 + SGL_RS11305 0.72 -0.1 1,999,110 - SGL_RS11305 0.73 -1.1 1,999,234 + SGL_RS11305 0.87 -0.8
Or see this region's nucleotide sequence