Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04920 and SGL_RS04925 are separated by 187 nucleotides SGL_RS04925 and SGL_RS04930 are separated by 26 nucleotides SGL_RS04930 and SGL_RS04935 are separated by 66 nucleotides SGL_RS04935 and SGL_RS04940 are separated by 153 nucleotides SGL_RS04940 and SGL_RS04945 are separated by 56 nucleotides
SGL_RS04920: SGL_RS04920 - tyrosine--tRNA ligase, at 659,370 to 660,584
_RS04920
SGL_RS04925: SGL_RS04925 - hypothetical protein, at 660,772 to 661,125
_RS04925
SGL_RS04930: SGL_RS04930 - hypothetical protein, at 661,152 to 662,819
_RS04930
SGL_RS04935: SGL_RS04935 - S4 domain-containing protein, at 662,886 to 663,188
_RS04935
SGL_RS04940: SGL_RS04940 - single-stranded DNA-binding protein, at 663,342 to 663,731
_RS04940
SGL_RS04945: SGL_RS04945 - GIY-YIG nuclease family protein, at 663,788 to 664,315
_RS04945
Position (kb) 661
662
663 Strain fitness (log2 ratio) -2
-1
0
1 at 661.040 kb on + strand, within SGL_RS04925 at 661.232 kb on + strand at 661.379 kb on - strand, within SGL_RS04930 at 661.499 kb on + strand, within SGL_RS04930 at 662.912 kb on + strand at 662.912 kb on + strand at 662.953 kb on - strand, within SGL_RS04935 at 663.023 kb on + strand, within SGL_RS04935 at 663.026 kb on + strand, within SGL_RS04935 at 663.096 kb on - strand, within SGL_RS04935 at 663.136 kb on + strand, within SGL_RS04935 at 663.262 kb on + strand at 663.668 kb on + strand, within SGL_RS04940
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 661,040 + SGL_RS04925 0.76 +0.4 661,232 + -1.2 661,379 - SGL_RS04930 0.14 -1.2 661,499 + SGL_RS04930 0.21 -2.0 662,912 + -0.7 662,912 + -0.7 662,953 - SGL_RS04935 0.22 -0.4 663,023 + SGL_RS04935 0.45 -1.0 663,026 + SGL_RS04935 0.46 -0.9 663,096 - SGL_RS04935 0.69 -1.6 663,136 + SGL_RS04935 0.83 -0.7 663,262 + +0.3 663,668 + SGL_RS04940 0.84 -0.3
Or see this region's nucleotide sequence