Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03855 and SGL_RS19775 are separated by 162 nucleotides SGL_RS19775 and SGL_RS03865 are separated by 34 nucleotides SGL_RS03865 and SGL_RS03870 are separated by 237 nucleotides
SGL_RS03855: SGL_RS03855 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 417,201 to 418,541
_RS03855
SGL_RS19775: SGL_RS19775 - TerB family tellurite resistance protein, at 418,704 to 419,126
_RS19775
SGL_RS03865: SGL_RS03865 - calcium/sodium antiporter, at 419,161 to 420,264
_RS03865
SGL_RS03870: SGL_RS03870 - histidinol dehydrogenase, at 420,502 to 421,731
_RS03870
Position (kb) 419
420
421 Strain fitness (log2 ratio) -1
0
1 at 418.193 kb on + strand, within SGL_RS03855 at 418.198 kb on + strand, within SGL_RS03855 at 418.198 kb on + strand, within SGL_RS03855 at 418.204 kb on - strand, within SGL_RS03855 at 418.206 kb on - strand, within SGL_RS03855 at 418.432 kb on + strand at 418.473 kb on - strand at 418.483 kb on - strand at 418.616 kb on + strand at 418.656 kb on + strand at 418.789 kb on + strand, within SGL_RS19775 at 418.907 kb on + strand, within SGL_RS19775 at 418.968 kb on + strand, within SGL_RS19775 at 418.970 kb on + strand, within SGL_RS19775 at 418.978 kb on - strand, within SGL_RS19775 at 418.978 kb on - strand, within SGL_RS19775 at 418.978 kb on - strand, within SGL_RS19775 at 418.978 kb on - strand, within SGL_RS19775 at 419.029 kb on + strand, within SGL_RS19775 at 419.040 kb on + strand, within SGL_RS19775 at 419.048 kb on - strand, within SGL_RS19775 at 419.048 kb on - strand, within SGL_RS19775 at 419.099 kb on + strand at 419.099 kb on + strand at 419.107 kb on - strand at 419.341 kb on - strand, within SGL_RS03865 at 419.414 kb on - strand, within SGL_RS03865 at 419.466 kb on - strand, within SGL_RS03865 at 419.894 kb on + strand, within SGL_RS03865 at 420.203 kb on + strand at 420.203 kb on + strand at 420.203 kb on + strand at 420.203 kb on + strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.211 kb on - strand at 420.306 kb on - strand at 420.313 kb on + strand at 420.321 kb on - strand at 420.353 kb on + strand at 420.376 kb on + strand at 420.407 kb on + strand at 420.430 kb on - strand at 420.442 kb on + strand at 420.464 kb on + strand at 420.466 kb on + strand at 420.474 kb on - strand at 420.559 kb on - strand at 420.653 kb on - strand, within SGL_RS03870 at 420.720 kb on + strand, within SGL_RS03870 at 420.728 kb on - strand, within SGL_RS03870 at 420.738 kb on - strand, within SGL_RS03870 at 420.865 kb on - strand, within SGL_RS03870 at 420.902 kb on - strand, within SGL_RS03870 at 420.921 kb on + strand, within SGL_RS03870 at 420.998 kb on + strand, within SGL_RS03870 at 421.026 kb on + strand, within SGL_RS03870 at 421.082 kb on - strand, within SGL_RS03870 at 421.228 kb on + strand, within SGL_RS03870 at 421.251 kb on - strand, within SGL_RS03870 at 421.251 kb on - strand, within SGL_RS03870
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 418,193 + SGL_RS03855 0.74 +0.5 418,198 + SGL_RS03855 0.74 +0.4 418,198 + SGL_RS03855 0.74 +0.6 418,204 - SGL_RS03855 0.75 -0.1 418,206 - SGL_RS03855 0.75 +0.3 418,432 + -0.3 418,473 - +0.8 418,483 - +0.3 418,616 + -0.5 418,656 + -0.8 418,789 + SGL_RS19775 0.20 -1.4 418,907 + SGL_RS19775 0.48 -0.4 418,968 + SGL_RS19775 0.62 +1.2 418,970 + SGL_RS19775 0.63 -0.4 418,978 - SGL_RS19775 0.65 -0.4 418,978 - SGL_RS19775 0.65 +0.5 418,978 - SGL_RS19775 0.65 -0.6 418,978 - SGL_RS19775 0.65 -0.8 419,029 + SGL_RS19775 0.77 -0.5 419,040 + SGL_RS19775 0.79 -0.2 419,048 - SGL_RS19775 0.81 -0.3 419,048 - SGL_RS19775 0.81 -0.7 419,099 + -0.1 419,099 + +0.2 419,107 - -0.5 419,341 - SGL_RS03865 0.16 -1.0 419,414 - SGL_RS03865 0.23 -0.5 419,466 - SGL_RS03865 0.28 -1.1 419,894 + SGL_RS03865 0.66 -0.8 420,203 + -0.3 420,203 + -0.6 420,203 + -0.6 420,203 + -0.4 420,211 - -1.0 420,211 - -1.1 420,211 - -0.0 420,211 - -1.5 420,306 - -1.1 420,313 + -0.7 420,321 - -0.6 420,353 + +1.2 420,376 + +0.3 420,407 + +0.4 420,430 - -0.8 420,442 + +0.2 420,464 + +0.6 420,466 + +0.4 420,474 - +0.3 420,559 - -0.1 420,653 - SGL_RS03870 0.12 -0.1 420,720 + SGL_RS03870 0.18 -0.5 420,728 - SGL_RS03870 0.18 +0.2 420,738 - SGL_RS03870 0.19 +0.4 420,865 - SGL_RS03870 0.30 +0.3 420,902 - SGL_RS03870 0.33 +0.4 420,921 + SGL_RS03870 0.34 -0.3 420,998 + SGL_RS03870 0.40 +0.6 421,026 + SGL_RS03870 0.43 -1.0 421,082 - SGL_RS03870 0.47 +1.6 421,228 + SGL_RS03870 0.59 +0.5 421,251 - SGL_RS03870 0.61 +0.1 421,251 - SGL_RS03870 0.61 +0.9
Or see this region's nucleotide sequence