Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03570 and SGL_RS03575 are separated by 151 nucleotides SGL_RS03575 and SGL_RS19770 are separated by 163 nucleotides SGL_RS19770 and SGL_RS03580 are separated by 4 nucleotides SGL_RS03580 and SGL_RS03585 are separated by 56 nucleotides
SGL_RS03570: SGL_RS03570 - Uma2 family endonuclease, at 357,748 to 358,608
_RS03570
SGL_RS03575: SGL_RS03575 - Uma2 family endonuclease, at 358,760 to 359,350
_RS03575
SGL_RS19770: SGL_RS19770 - DUF4160 domain-containing protein, at 359,514 to 359,663
_RS19770
SGL_RS03580: SGL_RS03580 - DUF2442 domain-containing protein, at 359,668 to 359,973
_RS03580
SGL_RS03585: SGL_RS03585 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 360,030 to 361,166
_RS03585
Position (kb) 358
359
360 Strain fitness (log2 ratio) -1
0
1 at 358.350 kb on + strand, within SGL_RS03570 at 358.357 kb on + strand, within SGL_RS03570 at 358.518 kb on + strand, within SGL_RS03570 at 358.545 kb on + strand at 358.553 kb on - strand at 359.135 kb on - strand, within SGL_RS03575 at 359.477 kb on - strand at 360.007 kb on - strand at 360.074 kb on + strand at 360.203 kb on - strand, within SGL_RS03585
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 358,350 + SGL_RS03570 0.70 -0.7 358,357 + SGL_RS03570 0.71 +0.5 358,518 + SGL_RS03570 0.89 +0.8 358,545 + -0.2 358,553 - -1.1 359,135 - SGL_RS03575 0.63 -0.5 359,477 - -1.2 360,007 - -1.0 360,074 + +0.2 360,203 - SGL_RS03585 0.15 -0.8
Or see this region's nucleotide sequence