Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03255 and SGL_RS03260 are separated by 112 nucleotides SGL_RS03260 and SGL_RS03265 are separated by 149 nucleotides SGL_RS03265 and SGL_RS03270 are separated by 194 nucleotides
SGL_RS03255: SGL_RS03255 - ATP-dependent 6-phosphofructokinase, at 293,878 to 294,963
_RS03255
SGL_RS03260: SGL_RS03260 - FUSC family protein, at 295,076 to 297,343
_RS03260
SGL_RS03265: SGL_RS03265 - photosystem II complex extrinsic protein PsbU, at 297,493 to 297,888
_RS03265
SGL_RS03270: SGL_RS03270 - HNH endonuclease, at 298,083 to 298,580
_RS03270
Position (kb) 295
296
297
298 Strain fitness (log2 ratio) -1
0
1 at 294.087 kb on - strand, within SGL_RS03255 at 294.123 kb on - strand, within SGL_RS03255 at 294.226 kb on - strand, within SGL_RS03255 at 294.232 kb on + strand, within SGL_RS03255 at 294.245 kb on - strand, within SGL_RS03255 at 294.261 kb on + strand, within SGL_RS03255 at 294.318 kb on + strand, within SGL_RS03255 at 294.577 kb on + strand, within SGL_RS03255 at 294.613 kb on + strand, within SGL_RS03255 at 294.682 kb on + strand, within SGL_RS03255 at 294.690 kb on - strand, within SGL_RS03255 at 294.752 kb on + strand, within SGL_RS03255 at 294.784 kb on - strand, within SGL_RS03255 at 294.880 kb on + strand at 294.912 kb on - strand at 295.090 kb on + strand at 295.194 kb on - strand at 295.196 kb on - strand at 295.275 kb on - strand at 295.275 kb on - strand at 295.305 kb on + strand, within SGL_RS03260 at 295.321 kb on + strand, within SGL_RS03260 at 295.341 kb on - strand, within SGL_RS03260 at 295.343 kb on - strand, within SGL_RS03260 at 295.412 kb on - strand, within SGL_RS03260 at 295.430 kb on + strand, within SGL_RS03260 at 295.445 kb on + strand, within SGL_RS03260 at 295.703 kb on - strand, within SGL_RS03260 at 295.903 kb on - strand, within SGL_RS03260 at 295.993 kb on - strand, within SGL_RS03260 at 296.054 kb on + strand, within SGL_RS03260 at 296.549 kb on - strand, within SGL_RS03260 at 296.711 kb on + strand, within SGL_RS03260 at 296.719 kb on - strand, within SGL_RS03260 at 296.719 kb on - strand, within SGL_RS03260 at 296.881 kb on + strand, within SGL_RS03260 at 297.288 kb on - strand at 297.780 kb on - strand, within SGL_RS03265 at 297.906 kb on - strand at 298.312 kb on - strand, within SGL_RS03270
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 294,087 - SGL_RS03255 0.19 +1.4 294,123 - SGL_RS03255 0.23 +0.0 294,226 - SGL_RS03255 0.32 -0.3 294,232 + SGL_RS03255 0.33 +0.6 294,245 - SGL_RS03255 0.34 -0.2 294,261 + SGL_RS03255 0.35 -0.2 294,318 + SGL_RS03255 0.41 -0.3 294,577 + SGL_RS03255 0.64 +0.1 294,613 + SGL_RS03255 0.68 +0.3 294,682 + SGL_RS03255 0.74 +0.5 294,690 - SGL_RS03255 0.75 -0.5 294,752 + SGL_RS03255 0.80 +0.2 294,784 - SGL_RS03255 0.83 -0.6 294,880 + +0.3 294,912 - -0.3 295,090 + +0.2 295,194 - -0.4 295,196 - +0.8 295,275 - -0.0 295,275 - -0.1 295,305 + SGL_RS03260 0.10 +0.3 295,321 + SGL_RS03260 0.11 +0.2 295,341 - SGL_RS03260 0.12 -0.4 295,343 - SGL_RS03260 0.12 -0.2 295,412 - SGL_RS03260 0.15 -0.7 295,430 + SGL_RS03260 0.16 -0.7 295,445 + SGL_RS03260 0.16 +0.3 295,703 - SGL_RS03260 0.28 -0.0 295,903 - SGL_RS03260 0.36 -0.3 295,993 - SGL_RS03260 0.40 -0.1 296,054 + SGL_RS03260 0.43 -0.3 296,549 - SGL_RS03260 0.65 -0.3 296,711 + SGL_RS03260 0.72 -0.2 296,719 - SGL_RS03260 0.72 -0.4 296,719 - SGL_RS03260 0.72 -0.5 296,881 + SGL_RS03260 0.80 -0.7 297,288 - -0.6 297,780 - SGL_RS03265 0.72 +1.0 297,906 - +0.5 298,312 - SGL_RS03270 0.46 +0.5
Or see this region's nucleotide sequence